Differentiation antigens on bovine mononuclear phagocytes identified by monoclonal antibodies

Ellis, John; Davis, William C.; MacHugh, Niall D.; Emery, D.L.; Kaushal, A.; Morrison, W. Ivan

doi:10.1016/0165-2427(88)90118-3

Cited by 61 publications

(27 citation statements)

References 21 publications

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“…To examine contaminating populations present in CD8+-enriched ELL, CD8+ T cells in the fraction were depleted by complement lysis with the CD8-specific mAb IL-A105 (IgG2a). Monocytes or dendritic cells were consistently absent from all ELL fractions, as determined by staining with mAb ILA24 (23).…”

Section: Methodsmentioning

confidence: 99%

Adoptive transfer of immunity to Theileria parva inthe CD8+ fraction of responding efferent lymph.

McKeever¹,

Taracha²,

Innes³

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

115

100

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Evidence that class I nuijor histocompatibility complex-restricted cytotoxic T lymphocytes (CTL) are involved in immunity to malaria has highlighted the potential importance of these cells in protection against intracellular parasites. Parasite-specific CTL are a prominent feature of the immune response of cattle to Theileria parva, a related apicomplexan parasite. The relationship between the appearance of these cells in the blood of immune cattle under challenge and the clearance of infection suggests that they are involved in the control of infection, but direct evidence is lacking that CTL can mediate protection. We have made a quantitative kinetic study of CTL responses in lymph originating from infected lymph nodes in a number of immune cattle under challenge with T. parva. Direct killing activity and the frequency of CTL precursors (CTLp) within responding cell populations were evaluated. A substantial increase in the proportion of CD8+ CTL was observed between days 8 and 11 after challenge. Frequencies of CTLp as high as 1:32 were observed and activity was essentially confined to the large blasting cell fraction. The analogous response in peripheral blood was oflower magnitude and delayed by 1-2 days. The high frequency of CTLp in efferent lymph permitted the adoptive transfer of this activity between immune and naive monozygotic twin calves. In separate experiments, naive calves lethally infected with T. parya were protected by inoculation of up to 1010 responding CD8+ T cells derived from their immune twins. Elimination of CD8+ T cells within the inoculum abrogated this effect. These rmnding provide direct evidence that CD8+ T cells can control T. parva infections in immune cattle.

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Section: Methodsmentioning

confidence: 99%

Adoptive transfer of immunity to Theileria parva inthe CD8+ fraction of responding efferent lymph.

McKeever¹,

Taracha²,

Innes³

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

115

100

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“…The following mAb, in addition to those described herein, were used: CH128A (IgG1) and IL-A43 (IgG2) are specific for bovine CD2 [19]; L A 5 7 (IgG2) is specific for an antigen expressed on mature bovine Tcells [20], believed to be the homologue of CD6 in man; IL-All (IgG2) and CC30 (IgG1) are specific for the bovine homologue of CD4 [ 171, and L A 6 7 (IgG1) and L A 1 7 (IgGI) are specific for the homologuesof CD5 and CD8, respectively [18, 211; =-A30 (IgGI) is specific for bovine IgM [26], and IL-A24 (IgG1) reacts with an antigen restricted to bovine monocytes and granulocytes [27].…”

Section: Staining Of Cell Suspension and Tissue Sectionsmentioning

confidence: 99%

Identification of a bovine surface antigen uniquely expressed on CD4⁻CD8⁻ T cell receptor γ/δ⁺ T lymphocytes

Clevers¹,

MacHugh²,

Bensaïd³

et al. 1990

Eur J Immunol

195

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In this study, two monoclonal antibodies, IL-A29 and CC15, are described that identify a novel bovine cell surface marker of 215/300 kDa. The antibodies reacted with a discrete population of resting lymphocytes in peripheral blood which, in young animals, constituted about 25% of the mononuclear cells. Thymus, lymph nodes and spleen contained less than 5% positive cells. These cells were negative for surface Ig, a monocyte/granulocyte marker, and the T lymphocyte antigens CD2, CD6, CD4 and CD8. Immunohistological analyses revealed the presence of IL-A29/CC15-positive lymphocytes in the thymic medulla, in the outer cortex of lymph nodes, in the marginal zones of the spleen, in the dermal and epidermal layers of the skin and in the lamina propria of the gut. The IL-A29/CC15+ cells in unfractionated blood mononuclear cells responded in autologous and allogeneic mixed lymphocyte cultures, and when purified they responded to concanavalin A in the presence of recombinant interleukin 2. These observations suggested this population of cells belonged to the T cell lineage. In order to unambiguously define their lineage, cDNA clones encoding bovine T cell receptor (TcR) and CD3 proteins were isolated. Northern blot analyses of IL-A29/CC15+ cell populations and of established cell lines of various lineages demonstrated that they expressed TcR delta and CD3 gamma, delta and epsilon mRNA: TcR alpha was not expressed, whereas only a truncated form of TcR beta mRNA was present. These results indicate that the IL-A29 and CC15 antibodies define a unique population of CD4-CD8-, gamma/delta T cells.

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“…The following MAbs were used: IL-A42, specific for bovine CD2 + cells (Teale et al, 1987); IL-All, specific for bovine CD4 + cells ; IL-A51, specific for bovine CD8 + cells (Ellis et al, 1986); IL-A24, specific for bovine monocytes and granulocytes (Ellis et al, 1988); IL-A29, specific for bovine CD4-, CDS-, ~ T cell receptor + cells 0'~ T cells; Clevers et al, 1990). These MAbs were produced at the International Laboratories for Research in Animal Diseases and obtained through the ATCC, with the exception of IL-A24 (generously supplied by Dr J.…”

Section: Methodsmentioning

confidence: 99%

Flow cytometric analysis of in vitro bluetongue virus infection of bovine blood mononuclear cells

Barratt‐Boyes¹,

Rossitto²,

Stott

et al. 1992

Journal of General Virology

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Differentiation antigens on bovine mononuclear phagocytes identified by monoclonal antibodies

Cited by 61 publications

References 21 publications

Adoptive transfer of immunity to Theileria parva inthe CD8+ fraction of responding efferent lymph.

Adoptive transfer of immunity to Theileria parva inthe CD8+ fraction of responding efferent lymph.

Identification of a bovine surface antigen uniquely expressed on CD4⁻CD8⁻ T cell receptor γ/δ⁺ T lymphocytes

Flow cytometric analysis of in vitro bluetongue virus infection of bovine blood mononuclear cells

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Differentiation antigens on bovine mononuclear phagocytes identified by monoclonal antibodies

Cited by 61 publications

References 21 publications

Adoptive transfer of immunity to Theileria parva inthe CD8+ fraction of responding efferent lymph.

Adoptive transfer of immunity to Theileria parva inthe CD8+ fraction of responding efferent lymph.

Identification of a bovine surface antigen uniquely expressed on CD4−CD8− T cell receptor γ/δ+ T lymphocytes

Flow cytometric analysis of in vitro bluetongue virus infection of bovine blood mononuclear cells

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Identification of a bovine surface antigen uniquely expressed on CD4⁻CD8⁻ T cell receptor γ/δ⁺ T lymphocytes