Differentiation-Arrested Rat Fetal Lung in Primary Monolayer Cell Culture. III. Antioxidant Enzyme Activity

Tanswell, A. Keith; Ba, Freeman

doi:10.3109/01902148409087902

Cited by 20 publications

(1 citation statement)

References 24 publications

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“…Growth of mixed monolayer cultures from fetal rat lung was also inhibited by hyperoxia [181-By contrast, type 2 pneumocytes from more mature fetuses were much more resistant to the effects of hyperoxia, and type 2 cells from newborn rat lung did not seem to be affected by exposure to 50% oxygen for 2 days. This is also true for fetal cells in monolayer cultures [19]. There were differences in the morphology of the cells in culture.…”

Section: Liiscussionmentioning

confidence: 77%

Effects of Hyperoxia on Surfactant Morphology and Cell Viability in Organotypic Cultures of Fetal Rat Lung

Loo

Smith

Lykke

1989

Experimental Lung Research

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Effects of hyperoxia in an organotypic model consisting of well-differentiated fetal rat type 2 pneumocytes have been studied by light and electron microscopy. In cultures exposed to 50% oxygen for 48 h, hyperoxia caused necrosis of cultured lung cells derived from 18- to 19-day gestation fetal rats, less damage in cells derived from 20- to 21-day gestation fetal rats, and no detectable damage of cells derived from newborn rats. After exposure to hyperoxia in organotypic cultures cocultured with fibroblast monolayers, ultrastructural abnormalities of surfactant (large lamellar bodies with disordered lamellae and abnormal shape) were detected in cells from 18- to 19-day gestation fetuses. These abnormalities were not noted when fibroblast monolayers were absent. Fibroblast conditioned medium from fibroblasts exposed to hyperoxia did not cause significant surfactant abnormalities at the ultrastructural level. These changes were less marked in cultures incubated with glutathione's constituent amino acids and with ascorbic acid during exposure to hyperoxia, and in cultures pretreated with dexamethasone (20 nM) before exposure to hyperoxia.

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Section: Liiscussionmentioning

confidence: 77%

Effects of Hyperoxia on Surfactant Morphology and Cell Viability in Organotypic Cultures of Fetal Rat Lung

Loo

Smith

Lykke

1989

Experimental Lung Research

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Age-Specific Difference in Pulmonary Cellular Injury and Mitochondrial Damage

Tuggle

Fanucchi

2014

Mitochondrial Function in Lung Health and Disease

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Differentiation-arrested rat fetal lung in primary monolayer cell culture

et al. 1984

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Differentiation-arrested lung cell cultures were developed from fetal rats of various gestational ages. In contrast to previously published observations with cultures in a pO2 of approximately 142 mm Hg, cultures developed in a pO2 of approximately 30 mm Hg, close to the normal fetal arterial pO2, have improved plating efficiency and a slightly increased growth rate. They did not, however, show gestation-dependent increases of choline incorporation into phospholipids, nor did immature lung cell cultures respond to dexamethasone or triiodothyronine, singly or in combination, by increased choline incorporation into saturated lecithin. The incorporation of choline and glycerol into lipids suggested a mature rate of lipid synthesis by immature cultures at a pO2 approximately 30 mm Hg, despite preservation of an immature morphology. Electron microscope observations revealed no gross differences between immature cultures developed at either pO2. The cellular mechanisms underlying these differences are unclear but suggest that oxygen tension may significantly influence results obtained with in vitro studies of lipid synthesis by immature lung.

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Differentiation-Arrested Rat Fetal Lung in Primary Monolayer Cell Culture. III. Antioxidant Enzyme Activity

Cited by 20 publications

References 24 publications

Effects of Hyperoxia on Surfactant Morphology and Cell Viability in Organotypic Cultures of Fetal Rat Lung

Effects of Hyperoxia on Surfactant Morphology and Cell Viability in Organotypic Cultures of Fetal Rat Lung

Age-Specific Difference in Pulmonary Cellular Injury and Mitochondrial Damage

Differentiation-arrested rat fetal lung in primary monolayer cell culture

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