In the last few years, the role of science in Cultural Heritage has assumed greater significance since diagnostics have become essential for the characterization of artworks. The development of conservation strategies involves growing the study of artworks and the knowledge of the materials used against the degradation plaguing the painted surfaces. This work focuses on the investigation of the degradation processes involving paintings on canvas, in particular delamination and progressive deterioration of the painted surfaces. The main causes of the degradation are attributable to the formation of metal soaps, which originate from the interaction between binders and pigments; as a result, the process leads to the progressive fracturing of the paint film. Using various characterization techniques allowed us to acquire information on the structural and morphological properties of the binder resins and study the binder/pigment interaction during the degradation process to understand the quantity and quality of the acid sites present in the binders and, consequently, the potential reactivity with the cationic part of the pigments. The binders were also analyzed within paint layers in contact with zinc oxide to study the interactions and the possible formation of new species as metal soaps and metal oxalates that can modify the boundary among the painting layers and, consequently, the appearance of the artwork and its artistic value. Modifications after UV and thermal aging processes were observed using Infrared spectroscopy and thermogravimetric analysis. Zinc soap formation was observed after 7 h of a UV aging process and was correlated to the acidity of the resins.