Differentiation between Pseudomonas testosteroni and P. acidovorans by gas chromatography

Brooks, John B.; Weaver, Robert E.; Tatum, H. W.; Billingsley, Sue A.

doi:10.1139/m72-226

Cited by 16 publications

(5 citation statements)

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“…testosteroni but not byP. acidovorans (4,15). Likewise, the presence of relatively large amounts of isobutyric (iC4) and isovaleric (iC5) acids clearly distinguishes P. pseudoalcaligenes from P. alcaligenes.…”

Section: Resultsmentioning

confidence: 99%

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Cellular fatty acids and metabolic products of Pseudomonas species obtained from clinical specimens

Moss¹,

Dees²

1976

J Clin Microbiol

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The cellular fatty acid composition of 112 reference strains and clinical isolates of Pseudomonas species was determined by gas-liquid chromatography (GLC). The presence and relative amounts of cyclopropane, hydroxy, and branched-chain fatty acids were distinguishing features of these strains. Determination of short-chain fatty acids extracted from spent growth media provided an additional means for identifying some strains. Our results show that clinical isolates of pseudomonads can be divided into eight distinct GLC groups. The procedures were especially useful for distinguishing glucose-nonoxidizing pseudomonads, which are difficult to identify by conventional criteria. Since the GLC procedures are simple, rapid, and highly reproducible, they are useful in diagnostic laboratories that process large numbers of cultures. Coupled with selected conventional tests, the analysis of short-chain and cellular fatty acids can be very useful for rapid screening of clinical isolates of Pseudomonas species.

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Section: Resultsmentioning

confidence: 99%

“…A major difference between these two groups is the presence of relatively 16:0 7:0 FIG. 4. Gas chromatograms of esterified fatty acids from saponified whole cells of P. acidovorans and P. pseudoalcaligenes.…”

Section: Resultsmentioning

confidence: 99%

Cellular fatty acids and metabolic products of Pseudomonas species obtained from clinical specimens

Moss¹,

Dees²

1976

J Clin Microbiol

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“…In addition to analyzing cells for fatty acids, the spent growth medium was also tested for short-chain acid products with a rapid GLC procedure which we developed (7). These compounds, although often present in smail amounts, provide valuable information for distinguishing closely related bacterial species (1,8,10,11). Shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Identification of Achromobacter species by cellular fatty acids and by production of keto acids

Dees¹,

Moss²

1978

J Clin Microbiol

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The cellular fatty acid composition and metabolic products of 12 reference strains of Achromobacter sp. and A. xylosoxidans were determined by gas-liquid chromatography (GLC). Results showed that the two Achromobacter groups are strikingly different and can be readily distinguished on the basis of cellular fatty acids and the short-chain acids produced by Achromobacter sp. The major cellular fatty acids of Achromobacter sp. were octadecenoic (18:1) and a 19-carbon cyclopropanoic (19:0 delta) acid, whereas hexadecanoic (16:0) and a 17-carbon cyclopropanoic (17:0 delta) acid were principal components of the lipids of A. xylosoxidans. Hydroxy acids were not found in strains of Achromobacter sp. but comprised approximately 20% of the cellular fatty acids of A. xylosoxidans. In addition, Achromobacter sp. produced relatively large amounts of 2-ketoisocaproic acid, which was detected in only trace amounts from strains of A. xylosoxidans. The data show that GLC tests provide additional criteria for differentiating groups which are very closely related when evaluated with conventional tests. The GLC tests can be readily adapted in the clinical laboratory because they are rapid, highly reproducible, relatively inexpensive, and simple to perform.

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“…For these reasons, a search for additional criteria which might lead to the development of rapid, specific, and reliable tests for distinguishing various Pseudomonas species was begun in this laboratory. Previous reports have shown that examination of cellular components and metabolic products provides useful new approaches to the taxonomy of certain of these organisms (2,5,9). This report describes the short-chain acids produced by Pseudomonas diminuta and P. vesiculare and shows that their analysis by gas-liquid chromatography (GLC) may provide a rapid, sensitive method for distinguishing the two species.…”

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confidence: 97%

Production of Glutaric Acid: a Useful Criterion for Differentiating Pseudomonas diminuta from Pseudomonas vesiculare

Moss¹,

Kaltenbach²

1974

Applied Microbiology

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Differentiation between Pseudomonas testosteroni and P. acidovorans by gas chromatography

Cited by 16 publications

References 0 publications

Cellular fatty acids and metabolic products of Pseudomonas species obtained from clinical specimens

Cellular fatty acids and metabolic products of Pseudomonas species obtained from clinical specimens

Identification of Achromobacter species by cellular fatty acids and by production of keto acids

Production of Glutaric Acid: a Useful Criterion for Differentiating Pseudomonas diminuta from Pseudomonas vesiculare

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