Differentiation between persistent infection/colonization and re-infection/re-colonization of Mycobacterium abscessus isolated from patients in Northeast Thailand

Kham-ngam, Irin; Chetchotisakd, Ploenchan; Ananta, Pimjai; Chaimanee, Prajaub; Reechaipichitkul, Wipa; Lulitanond, Viraphong; Namwat, Wises; Faksri, Kiatichai

doi:10.1016/j.meegid.2018.12.001

Cited by 13 publications

(15 citation statements)

References 34 publications

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“… a Subspecies of M . abscessus were identified based on MLST as in a previous study [ 41 ]. b The DST was performed following the method that is described above and types of CLA resistance were interpreted based on in vitro MIC results.…”

Section: Resultsmentioning

confidence: 99%

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Assessment of in vitro activities of novel modified antimicrobial peptides against clarithromycin resistant Mycobacterium abscessus

et al. 2021

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Mycobacterium abscessus (Mab) is one of the most drug resistant bacteria with a high treatment failure rate. Antimicrobial peptides (AMPs) are alternative therapeutic agents against this infection. This study was aimed to assess the in vitro activities of thirteen AMPs (S5, S52, S6, S61, S62, S63, KLK, KLK1, KLK2, Pug-1, Pug-2, Pug-3 and Pug-4) that have never been investigated against drug resistant Mab isolates. Only four novel modified AMPs (S61, S62, S63 and KLK1) provided the lowest minimum inhibitory concentration (MIC) values ranging from 200–400 μg/ml against the Mab ATCC19977 strain. These four potential AMPs were further tested with 16 clinical isolates of clarithromycin resistant Mab. The majority of the tested strains (10/16 isolates, 62.5%) showed ~99% kill by all four AMPs within 24 hours with an MIC <50 μg/ml. Only two isolates (12.5%) with acquired clarithromycin resistance, however, exhibited values <50 μg/ml of four potential AMPs, S61, S62, S63 and KLK1 after 3-days-incubation. At the MICs level, S63 showed the lowest toxicity with 1.50% hemolysis and 100% PBMC viability whereas KLK1 showed the highest hemolysis (10.21%) and lowest PBMC viability (93.52%). S61, S62 and S63 were further tested with clarithromycin-AMP interaction assays and found that 5/10 (50%) of selected isolates exhibited a synergistic interaction with 0.02–0.41 FICI values. This present study demonstrated the potential application of novel AMPs as an adjunctive treatment with clarithromycin against drug resistant Mab infection.

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Section: Resultsmentioning

confidence: 99%

“…Genomic DNA of Mab isolates were extracted from loops full of colonies using the cetyl-trimethyl-ammonium bromide-sodium chloride (CTAB) method [ 40 ]. Subspecies of Mab were identified based on multilocus sequence typing (MLST) as in a previous study [ 41 ]. Informed consent was not required for this study.…”

Section: Methodsmentioning

confidence: 99%

Assessment of in vitro activities of novel modified antimicrobial peptides against clarithromycin resistant Mycobacterium abscessus

et al. 2021

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“…The colony morphology of each isolate was noted. The identification of M. abscessus species was performed according to the protocol previously published ( Kham-Ngam et al, 2019 ). All isolates were sub-cultured on Lowenstein-Jensen (LJ) media and incubated at 37 °C for 7 days before further analysis.…”

Section: Methodsmentioning

confidence: 99%

“…The minimum inhibitory concentration (MIC) for clarithromycin was determined according to published protocols ( Kham-Ngam et al, 2019 ). The broth-microdilution method using a RAPMYCOI Sensititre 96-well plate (TREK Diagnostic Systems, Independence, OH, USA) following the manufacturer’s protocol.…”

Section: Methodsmentioning

confidence: 99%

Assessment of antimycobacterial activities of pure compounds extracted from Thai medicinal plants against clarithromycin-resistant Mycobacterium abscessus

Sirichoat

Kham-ngam

Kaewprasert

et al. 2021

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Background Infection with Mycobacterium abscessus is usually chronic and is associated with clarithromycin resistance. Increasing drug resistance is a major public-health problem and has led to the search for new antimycobacterial agents. We evaluated the antimycobacterial activity, toxicity, and synergistic effects of several plant secondary metabolites against M. abscessus. Methods Twenty-three compounds were evaluated for antimycobacterial activity against thirty M. abscessus clinical isolates by broth microdilution to determine their minimum inhibitory concentration (MIC) values. Toxicity was evaluated using red and white blood cells (RBCs and WBCs). The compounds were used in combination with clarithromycin to investigate the possibility of synergistic activity. Results Five out of twenty-three compounds (RL008, RL009, RL011, RL012 and RL013) exhibited interesting antimycobacterial activity against M. abscessus, with MIC values ranging from <1 to >128 μg/mL. These extracts did not induce hemolytic effect on RBCs and displayed low toxicity against WBCs. The five least-toxic compounds were tested for synergism with clarithromycin against seven isolates with inducible clarithromycin resistance and seven with acquired clarithromycin resistance. The best synergistic results against these isolates were observed for RL008 and RL009 (8/14 isolates; 57%). Conclusions This study demonstrated antimycobacterial and synergistic activities of pure compounds extracted from medicinal plants against clarithromycin-resistant M. abscessus. This synergistic action, together with clarithromycin, may be effective for treating infections and should be further studied for the development of novel antimicrobial agents.

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“…Genomic DNA of Mab isolates were extracted from loops full of colonies using the cetyltrimethyl-ammonium bromide-sodium chloride (CTAB) method [40]. Subspecies of Mab were identified based on multilocus sequence typing (MLST) as in a previous study [41]. Informed consent was not required for this study.…”

Section: Culture Identification and Dna Extraction From Mab Isolatesmentioning

confidence: 99%

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Differentiation between persistent infection/colonization and re-infection/re-colonization of Mycobacterium abscessus isolated from patients in Northeast Thailand

Cited by 13 publications

References 34 publications

Assessment of in vitro activities of novel modified antimicrobial peptides against clarithromycin resistant Mycobacterium abscessus

Assessment of in vitro activities of novel modified antimicrobial peptides against clarithromycin resistant Mycobacterium abscessus

Assessment of antimycobacterial activities of pure compounds extracted from Thai medicinal plants against clarithromycin-resistant Mycobacterium abscessus

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