Differentiation between probiotic and wild-type Bacillus cereus isolates by antibiotic susceptibility test and Fourier transform infrared spectroscopy (FT-IR)

Mietke, Henriette; Beer, W.; Schleif, J.; Schabert, G.; Reissbrodt, Rolf

doi:10.1016/j.ijfoodmicro.2010.02.009

Cited by 26 publications

(13 citation statements)

References 9 publications

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“…Fourier transform infrared spectroscopy is a powerful tool for microbial diagnostics and epidemiological studies and has already been successfully used to type B. cereus group strains (Ehling-Schulz et al, 2005b; Mietke et al, 2010). Basically, FTIR is a vibrational spectroscopic technique, which is able to distinguish microbial cells at different taxonomic levels (Naumann et al, 1991; Wenning et al, 2008).…”

Section: Population Studies and Contamination Route Analysismentioning

confidence: 99%

Bacillus “next generation” diagnostics: moving from detection toward subtyping and risk-related strain profiling

Ehling‐Schulz¹,

Messelhäußer²

2013

Front. Microbiol.

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The highly heterogeneous genus Bacillus comprises the largest species group of endospore forming bacteria. Because of their ubiquitous nature, Bacillus spores can enter food production at several stages resulting in significant economic losses and posing a potential risk to consumers due the capacity of certain Bacillus strains for toxin production. In the past, food microbiological diagnostics was focused on the determination of species using conventional culture-based methods, which are still widely used. However, due to the extreme intra-species diversity found in the genus Bacillus, DNA-based identification and typing methods are gaining increasing importance in routine diagnostics. Several studies showed that certain characteristics are rather strain-dependent than species-specific. Therefore, the challenge for current and future Bacillus diagnostics is not only the efficient and accurate identification on species level but also the development of rapid methods to identify strains with specific characteristics (such as stress resistance or spoilage potential), trace contamination sources, and last but not least discriminate potential hazardous strains from non-toxic strains.

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Section: Population Studies and Contamination Route Analysismentioning

confidence: 99%

Bacillus “next generation” diagnostics: moving from detection toward subtyping and risk-related strain profiling

Ehling‐Schulz¹,

Messelhäußer²

2013

Front. Microbiol.

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“…This could be shown also by other phenotypic tests like Fourier transform infrared spectroscopy (11). Therefore, MLST has a high differenti- ating potential and together with PFGE can be used for further studies to improve knowledge of the characteristics of probiotic B. cereus strains.…”

Section: Discussionmentioning

confidence: 93%

“…To avoid mislabeling or introduction of enterotoxin genes of food poisoning Bacillus strains, the taxonomic unit must be described (10) and a strain-specific differentiation must be done as well. Fingerprinting methods have been applied successfully for such purposes (11,15); however, for probiotic Bacillus strains mainly pulsed-field gel electrophoresis (PFGE) has been applied (3). Furthermore, PFGE was proposed and adopted for official control as the method for identification at strain level of the probiotic Bacillus strains authorized as feed additives in the EU (12).…”

mentioning

confidence: 99%

Molecular Characterization of the Probiotic Strain Bacillus cereus var. toyoi NCIMB 40112 and Differentiation from Food Poisoning Strains

Klein

2011

Journal of Food Protection

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Bacillus cereus var. toyoi strain NCIMB 40112 (Toyocerin), a probiotic authorized in the European Union as feed additive for swine, bovines, poultry, and rabbits, was characterized by DNA fingerprinting applying pulsed-field gel electrophoresis and multilocus sequence typing and was compared with reference strains (of clinical and environmental origins). The probiotic strain was clearly characterized by pulsed-field gel electrophoresis using the restriction enzymes Apa I and Sma I resulting in unique DNA patterns. The comparison to the clinical reference strain B. cereus DSM 4312 was done with the same restriction enzymes, and again a clear differentiation of the two strains was possible by the resulting DNA patterns. The use of the restriction enzymes Apa I and Sma I is recommended for further studies. Furthermore, multilocus sequence typing analysis revealed a sequence type (ST 111) that was different from all known STs of B. cereus strains from food poisoning incidents. Thus, a strain characterization and differentiation from food poisoning strains for the probiotic strain was possible.

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“…It was possible to separate probiotic B. cereus strains from wild-type B. cereus , B. thuringiensis , B. mycoides , B. weihenstephanensis using HCA. The main differences were observed in the fatty acid composition (3000–2800 cm −1 region), carbohydrates (1200–900 cm −1 region) and fingerprint region (900–700 cm −1 ) [67]. …”

Section: Applicationsmentioning

confidence: 99%

Applications of Infrared and Raman Spectroscopies to Probiotic Investigation

Santos

Gerbino

Tymczyszyn

et al. 2015

Foods

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In this review, we overview the most important contributions of vibrational spectroscopy based techniques in the study of probiotics and lactic acid bacteria. First, we briefly introduce the fundamentals of these techniques, together with the main multivariate analytical tools used for spectral interpretation. Then, four main groups of applications are reported: (a) bacterial taxonomy (Subsection 4.1); (b) bacterial preservation (Subsection 4.2); (c) monitoring processes involving lactic acid bacteria and probiotics (Subsection 4.3); (d) imaging-based applications (Subsection 4.4). A final conclusion, underlying the potentialities of these techniques, is presented.

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Differentiation between probiotic and wild-type Bacillus cereus isolates by antibiotic susceptibility test and Fourier transform infrared spectroscopy (FT-IR)

Cited by 26 publications

References 9 publications

Bacillus “next generation” diagnostics: moving from detection toward subtyping and risk-related strain profiling

Bacillus “next generation” diagnostics: moving from detection toward subtyping and risk-related strain profiling

Molecular Characterization of the Probiotic Strain Bacillus cereus var. toyoi NCIMB 40112 and Differentiation from Food Poisoning Strains

Applications of Infrared and Raman Spectroscopies to Probiotic Investigation

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