Differentiation between spore-forming and asporogenic bacteria using a PCR and Southern hybridization based method

Brill, Julia A.; Wiegel, Juergen

doi:10.1016/s0167-7012(97)00091-2

Cited by 43 publications

(26 citation statements)

References 38 publications

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“…A photomicrograph of sporangial morphology, with a bar marker for size, should be provided, and details of sporulation conditions should be given. Sporulation has not been observed in several recently described species, but the potential to form endospores may be detected using a PCR method based upon certain genes for sporulation (Brill & Wiegel, 1997).…”

Section: Sporangiamentioning

confidence: 99%

Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria

Logan

Berge

Bishop

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

444

290

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Minimal standards for describing new taxa within the aerobic endospore-forming bacteria are proposed, following Recommendation 30b of the Bacteriological Code (1990 Revision). These minimal standards are recommended as guidelines to assist authors in the preparation of descriptions for novel taxa. They encourage broad polyphasic characterization and the construction of descriptions that are practically useful in routine diagnostic laboratories. The proposals have been endorsed by the Subcommittee on the Taxonomy of the Genus Bacillus and Related Organisms of the International Committee on Systematics of Prokaryotes

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Section: Sporangiamentioning

confidence: 99%

Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria

Logan

Berge

Bishop

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

444

290

View full text Add to dashboard Cite

show abstract

“…In the PCR determination of sporulation genes, the targeted segments of spoOA were consistently amplified under a range conditions as described by Brill & Wiegel (1997) ssp gene sequences was obtained. On the basis of these negative results for sporulation, the new isolate appeared to be a non-spore-forming bacterium.…”

Section: Morphologymentioning

confidence: 99%

“…The spore-forming ability of the new isolate was examined by physiological induction under various growth conditions and using molecular determination of sporulation genes (Brill & Wiegel, 1997). Cells were cultivated to early exponential growth phase under optimal conditions and then exposed to lower (60, 55,50 and 45 "C) or higher temperatures (80 and 85 "C).…”

Section: Introductionmentioning

confidence: 99%

“…The molecular determination of spoOA and ssp genes, which are involved in sporulation of many endospore-forming bacteria and have been used as molecular probes for the screening of spore-forming bacteria (Brown et al, 1994;Setlow, 1988;Mohr et al, 1991 ;Brill & Wiegel, 1997), was performed by PCR. The primers for amplification were designed and the PCR reactions were carried out as described by Brill & Wiegel (1997).…”

Section: Introductionmentioning

confidence: 99%

“…The primers for amplification were designed and the PCR reactions were carried out as described by Brill & Wiegel (1997). All PCR products were directly sequenced on both strands by the dideoxynucleotide chain termination method using DNA sequencer model 373As (Perkin Elmer/Applied Biosystems).…”

Section: Introductionmentioning

confidence: 99%

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Thermaerobacter marianensis gen. nov., sp. nov., an aerobic extremely thermophilic marine bacterium from the 11000 m deep Mariana Trench

Takai

Inoue

Horikoshi

1999

International Journal of Systematic and Evolutionary Microbiology

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A novel extremely thermophilic bacterium was isolated from the world's deepest sea-floor, the Mariana Trench Challenger Deep a t a depth of 10897 m. Cells were Gram-reaction variable, non-spore-forming and non-motile rods without flagella. Growth was observed between 50 and 80 "C (optimum: 74-76 "C; 90 min doubling time), pH 5 4 and 9.5 (optimum: pH 7.0-705) and 0 5 and 5 % sea salts (optimum: 2 % sea salts). The isolate was a strictly aerobic heterotroph capable of utilizing as sole energy and carbon source: yeast extract, peptone, cellulose, starch, chitin, casein, Casamino acids, a variety of sugars, carboxylic acids and amino acids. The G+C content of the genomic DNA was 72.5 mol%. Phylogenetic analysis based on 165 rRNA sequences placed this aerobic, high-G+C-content bacterium among the members of the Grampositive, low-G+C-content anaerobic thermophilic bacteria within the Bacillus-Clostridium subphylum. On the basis of the physiological and molecular properties of the new isolate, the name Thermaembacter marianensis gen. nov., sp. nov. (type strain 7p75aT = JCM 102463 is proposed.

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Thermophiles: Anaerobic Alkalithermophiles

Wiegel

2003

Encyclopedia of Environmental Microbiology

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Definition of “Extreme” Mesophilic and Thermophilic Alkaliphiles have been Studied Only Recently Alkalithermophiles Thermophilic “Superbugs” Among Validly Described Species of Extremophiles Biogeography and Biogeochemistry Some Comments on Methods for Studying Anaerobic Alkalithermophiles Anaerobic Alkalithermophilic Bacteria and their Habitats Known Thus Far Archaeal Species and their Known Habitats Possible Correlation between the Shortest Observed Doubling Times for a Species and the Type of Habitat from Which the Alkalithermophiles were Isolated

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Differentiation between spore-forming and asporogenic bacteria using a PCR and Southern hybridization based method

Cited by 43 publications

References 38 publications

Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria

Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria

Thermaerobacter marianensis gen. nov., sp. nov., an aerobic extremely thermophilic marine bacterium from the 11000 m deep Mariana Trench

Thermophiles: Anaerobic Alkalithermophiles

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