Differentiation between two strains of microalga Parachlorella kessleri using modern spectroscopic method

Saleh, Marwa M.; Маторин, Д. Н.; Zayadan, B. K.; Todorenko, D. A.; Lukashov, Evgenii P; Gaballah, Mona

doi:10.1186/s40529-014-0053-7

Cited by 5 publications

(2 citation statements)

References 21 publications

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“…The chlorococcal alga Parachlorella kessleri (formerly Chlorella kessleri [ 19 ]) is a biotechnologically promising algal species [ 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 ] that divides by multiple fission. Its cell cycle consists of multiple rounds of DNA replication that, after completion of the last one, are followed by successive nuclear divisions.…”

Section: Introductionmentioning

confidence: 99%

Supra-Optimal Temperature: An Efficient Approach for Overaccumulation of Starch in the Green Alga Parachlorella kessleri

Zachleder

Kselíková

Ivanov

et al. 2021

Cells

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Green algae are fast-growing microorganisms that are considered promising for the production of starch and neutral lipids, and the chlorococcal green alga Parachlorella kessleri is a favorable model, as it can produce both starch and neutral lipids. P. kessleri commonly divides into more than two daughter cells by a specific mechanism—multiple fission. Here, we used synchronized cultures of the alga to study the effects of supra-optimal temperature. Synchronized cultures were grown at optimal (30 °C) and supra-optimal (40 °C) temperatures and incident light intensities of 110 and 500 μmol photons m−2 s−1. The time course of cell reproduction (DNA replication, cellular division), growth (total RNA, protein, cell dry matter, cell size), and synthesis of energy reserves (net starch, neutral lipid) was studied. At 40 °C, cell reproduction was arrested, but growth and accumulation of energy reserves continued; this led to the production of giant cells enriched in protein, starch, and neutral lipids. Furthermore, we examined whether the increased temperature could alleviate the effects of deuterated water on Parachlorella kessleri growth and division; results show that supra-optimal temperature can be used in algal biotechnology for the production of protein, (deuterated) starch, and neutral lipids.

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Section: Introductionmentioning

confidence: 99%

Supra-Optimal Temperature: An Efficient Approach for Overaccumulation of Starch in the Green Alga Parachlorella kessleri

Zachleder

Kselíková

Ivanov

et al. 2021

Cells

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“…The difference in the laser power necessary to close most PSII thus seems to be primarily attributable to the difference in the absorption coefficient at 404 nm for PSII. In P. kessleri, as one of green algae, PSII possess an antenna system that contains Chla and chlorophyll b (Chlb) as main antenna pigments exhibiting strong absorption mainly in the blue-green and red regions [80,81]. For the simulation, we have assumed the same molecular organization of PSII supercomplex as in C. reinhardtii [67], the total absorption coefficient per PSII monomer in state 1 (Text S2) is equivalent to about 191 Chla molecules per monomer PSII (Fig.…”

Section: Transition From Open To Closed State Of Psii As Observed By ...mentioning

confidence: 99%

Characterization of thylakoid membrane in a heterocystous cyanobacterium and green alga with dual-detector fluorescence lifetime imaging microscopy with a systematic change of incident laser power

Nozue

Mukuno

Tsuda

et al. 2016

Biochimica et Biophysica Acta (BBA) - Bioenergetics

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Fluorescence Lifetime Imaging Microscopy (FLIM) has been applied to plants, algae and cyanobacteria, in which excitation laser conditions affect the chlorophyll fluorescence lifetime due to several mechanisms. However, the dependence of FLIM data on input laser power has not been quantitatively explained by absolute excitation probabilities under actual imaging conditions. In an effort to distinguish between photosystem I and photosystem II (PSI and PSII) in microscopic images, we have obtained dependence of FLIM data on input laser power from a filamentous cyanobacterium Anabaena variabilis and single cellular green alga Parachlorella kessleri. Nitrogen-fixing cells in A. variabilis, heterocysts, are mostly visualized as cells in which short-lived fluorescence (≤0.1 ns) characteristic of PSI is predominant. The other cells in A. variabilis (vegetative cells) and P. kessleri cells show a transition in the status of PSII from an open state with the maximal charge separation rate at a weak excitation limit to a closed state in which charge separation is temporarily prohibited by previous excitation(s) at a relatively high laser power. This transition is successfully reproduced by a computer simulation with a high fidelity to the actual imaging conditions. More details in the fluorescence from heterocysts were examined to assess possible functions of PSII in the anaerobic environment inside the heterocysts for the nitrogen-fixing enzyme, nitrogenase. Photochemically active PSII:PSI ratio in heterocysts is tentatively estimated to be typically below our detection limit or at most about 5% in limited heterocysts in comparison with that in vegetative cells.

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Microalgal consortia technology: A novel and sustainable approach of resource reutilization, waste management and lipid production

Upadhyay

Singh

et al. 2021

Environmental Technology & Innovation

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Differentiation between two strains of microalga Parachlorella kessleri using modern spectroscopic method

Cited by 5 publications

References 21 publications

Supra-Optimal Temperature: An Efficient Approach for Overaccumulation of Starch in the Green Alga Parachlorella kessleri

Supra-Optimal Temperature: An Efficient Approach for Overaccumulation of Starch in the Green Alga Parachlorella kessleri

Characterization of thylakoid membrane in a heterocystous cyanobacterium and green alga with dual-detector fluorescence lifetime imaging microscopy with a systematic change of incident laser power

Microalgal consortia technology: A novel and sustainable approach of resource reutilization, waste management and lipid production

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