Differentiation by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) of Candida albicans isolated from upper respiratory tract in patients with non-small cell lung cancer.

Biernasiuk, Anna; Korona-Głowniak, Izabela; Grzegorczyk, Agnieszka; Malm, Anna

doi:10.18388/abp.2014_1837

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…As the resistance of C. albicans to antifungal drugs commonly used in local and general treatment is increasing, it is necessary to look for simple and adverse affects-free prophylactic and therapeutic means to counteract the increase (11). In the case of oral cavity mycoses, an alternative to conventional antimycotics may be chlorhexidine which, as research confirmed, shows antifungal activity in vivo and in vitro (25).…”

Section: Discussionmentioning

confidence: 99%

Antifungal drugs resistance profiles of C. albicans strains isolated from the oral cavity of children and adolescents

Kamińska¹,

Malm²,

Szymańska³

2019

Acta Poloniae Pharmaceutica - Drug Research

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Fungi, mainly yeast-like fungi of the Candida genus, and especially the species Candida albicans, occupy an important place among microorganisms that colonize the oral cavity. The aim of this paper was to assess the susceptibility of different strains to some antifungal drugs. The study material consisted of swab samples taken from the buccal mucosa of 376 healthy individuals of both genders, from neonate to18 years. C. albicans strains were isolated in 79 persons. To evaluate drug resistance phenotypes of the isolated C. albicans strains Fungitest TM (Bio-Rad) was used. The study showed that in different age groups the percentage of the tested C. albicans isolates susceptible to all the tested azole drugs varied from 76% to 95%. Drug-resistant strains were found only in the eldest age group (4.8%). Phenotypic diversity (drug resistance types) of the studied isolates indicates a wide range of adaptation possibilities enabling colonization of various ecological niches in the oral cavity ecosystem. The presence of C. albicans strains intermediate or resistant to azole drugs among the tested isolates may be due to the overuse of that drug group in general therapy and the oral cavity treatment.

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Section: Discussionmentioning

confidence: 99%

Antifungal drugs resistance profiles of C. albicans strains isolated from the oral cavity of children and adolescents

Kamińska¹,

Malm²,

Szymańska³

2019

Acta Poloniae Pharmaceutica - Drug Research

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“…Cancer patients are predisposed to fungal infections caused by Candida albicans , especially oral or respiratory tract candidiasis. Biernasiuk and colleagues [ 30 ] applied RAPD-PCR to successfully estimate genetic diversity of C. albicans isolated from the upper respiratory tract of patients with non-small cell lung cancer. However, there are some disadvantages to RAPD, like poor reproducibility and low yield of PCR products (low production).…”

Section: Discussionmentioning

confidence: 99%

Development of diagnostic SCAR markers for genomic DNA amplifications in breast carcinoma by DNA cloning of high-GC RAMP-PCR fragments

Cheng

Wei

et al. 2017

Oncotarget

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Cancer is genetically heterogeneous regarding to molecular genetic characteristics and pathogenic pathways. A wide spectrum of biomarkers, including DNA markers, is used in determining genomic instability, molecular subtype determination and disease prognosis, and estimating sensitivity to different drugs in clinical practice. In a previous study, we developed highly effective DNA markers using improved random amplified polymorphic DNA (RAPD) with high-GC primers, which is a valuable approach for the genetic authentication of medicinal plants. In this study, we applied this effective DNA marker technique to generate genetic fingerprints that detect genomic alterations in human breast cancer tissues and then developed sequence-characterized amplified region (SCAR) markers. Three SCAR markers (BC10-1, BC13-4 and BC31-2) had high levels of genomic DNA amplification in breast cancer. The PHKG2 and RNF40 genes are either overlapping or close to the sequences of SCAR marker BC13-4, while SCAR marker BC10-1 is in the intron and overlap the DPEP1 gene, suggesting that alterations in the expression of these genes could contribute to cancer progression. Screening of breast cancer cell lines showed that the mRNA expression levels for the PHKG2 and DPEP1 were lower in non-tumorigenic mammary epithelial cell MCF10A, but elevated in other cell lines. The DPEP1 mRNA level in invasive ductal carcinoma specimens was significantly higher than that of the adjacent normal tissues in women. Taken together, high-GC RAMP-PCR provides greater efficacy in measuring genomic DNA amplifications, deletion or copy number variations. Furthermore, SCAR markers BC10-1 and BC13-4 might be useful diagnostic markers for breast cancer carcinomas.

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Random amplified polymorphic DNA -RAPD analysis of human cervical cancer in Samwha city

Abdulwahab Ati Al-Askeri

2023

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Objectives: The study intended to distinguish a likely hereditary variety in the genomic DNA of Iraqi patients corresponding with cervical disease and the possibility of getting an advantage of the potentially produced DNA fragment(s) as a molecular test for future related applications. Methods: Blood and tissue tests were collected from female patients with cervical malignant growth and solid females; DNA was extricated and purged from each example, and then "Irregular Amplification of Polymorphic DNA technique"(RAPD) was directed with oligonucleotide "OPA-20". Results: RAPD results examination with oligonucleotide “OPA-20” could identify four polymorphic DNA groups in the genome of patients females, in which these bands were missing in the majority of genomic DNA of healthy people. Keywords: Tumor, PCR, Iraq, gene polymorphism

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Differentiation by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) of Candida albicans isolated from upper respiratory tract in patients with non-small cell lung cancer.

Cited by 3 publications

References 13 publications

Antifungal drugs resistance profiles of C. albicans strains isolated from the oral cavity of children and adolescents

Antifungal drugs resistance profiles of C. albicans strains isolated from the oral cavity of children and adolescents

Development of diagnostic SCAR markers for genomic DNA amplifications in breast carcinoma by DNA cloning of high-GC RAMP-PCR fragments

Random amplified polymorphic DNA -RAPD analysis of human cervical cancer in Samwha city

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