Posterior tibial slope (PTS) is important for the success of high tibial osteotomy and unicondylar knee arthroplasty applications, as it provides anterior cruciate ligament function. In the literature, different studies have been carried out with various imaging methods to measure PTS in populations of different ethnic origins. In this study, it was aimed to detect PTS in the medial (MPTS) and lateral (LPTS) tibial condyles with computed tomography in a Turkish population sample and to compare the results between age groups (<65, ≥ 65), genders, sides, and literature data. In our sample, 39 left and 33 right knee images of 37 men and 35 women with a mean age of 52.01 ± 21.27 were evaluated. The tibial proximal anatomical axis was determined by the midpoint method. The MPTS and LPTS was evaluated by two different observers according to this axis. Thereby the global PTS (GPTS) was calculated as an arithmetic mean of MPTS and LPTS values. Measurements were repeated 2 weeks after the first measurement and values were analyzed. A significant difference was found between the mean of MPTS, LPTS, and GPTS in the whole population (
P
= .002), in men (
P
= .02) and in women (
P
= .02). On the other hand, there was no significant difference compared according to age, gender, and side by mean of same parameters. In comparison of the results of our Turkish population sample with other studies in the literature, MPTS and LPTS were similar to Chinese (
P
= .22,
P
= .07) and Japanese (
P
= .96,
P
= .67) populations, while different to White Asian (
P
< .001,
P
< .001) and Korean (
P
< .001,
P
< .001) populations. The midpoint method is a safe measurement method in computed tomography-based studies for the evaluation of PTS. Implant designs produces for different populations may not be suitable for the Turkish population. More comprehensive and detailed studies are needed to represent the Turkish population