Differentiation of canine adipose tissue–derived mesenchymal stem cells towards endothelial progenitor cells

Kang, Byung Jae; Lee, Jun Young; Kweon, Oh‐Kyeong; Cho, Je Yoel

doi:10.2460/ajvr.75.7.685

Cited by 12 publications

(10 citation statements)

References 27 publications

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“…The effect of CCM exosomes has been evaluated in some cell types [ 30 ]; however, it has never been assessed in mesenchymal stem cells (MSCs). MSCs play a strategic role in the development, homeostasis and repair of different organs and tissues [ 31 , 32 ] and have shown promising results in the treatment of different canine diseases [ 33 , 34 ]. Therefore, we believe that it is interesting to demonstrate the effect of canine colostrum exosomes on different types of MSCs to help understand their role in the neonatal period in the canine species.…”

Section: Introductionmentioning

confidence: 99%

Canine colostrum exosomes: characterization and influence on the canine mesenchymal stem cell secretory profile and fibroblast anti-oxidative capacity

et al. 2020

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Background Canine colostrum milk (CCM) is a specific secretion of the mammary gland that is fundamental for the survival of the newborn. CCM has many described components (immunoglobulins, proteins or fat), but its small vesicles, named exosomes, are largely unknown. Results A characterization of CCM exosomes was performed. Exosomes were abundant in CCM and appeared with the characteristic cup-shaped morphology and well-defined round vesicles. The size distribution of exosomes was between 37 and 140 nm, and western blot analysis showed positive expression of specific exosomal markers. Proteomic analysis revealed a total of 826 proteins in exosome cargo. We also found that exosomes modified the proliferation and secretory profiles in canine mesenchymal stem cells derived from bone marrow (cBM-MSCs) and adipose tissue (cAd-MSCs). Additionally, CCM exosomes demonstrated a potent antioxidant effect on canine fibroblasts in culture. Conclusions Our findings highlight, for the first time, the abundant presence of exosomes in CCM and their ability to interact with mesenchymal stem cells (MSCs). The addition of exosomes to two types of MSCs in culture resulted in specific secretory profiles with functions related to angiogenesis, migration and chemotaxis of immune cells. In particular, the cAd-MSCs secretory profile showed higher potential in adipose tissue development and neurogenesis, while cBM-MSC production was associated with immunity, cell mobilization and haematopoiesis. Finally, exosomes also presented antioxidant capacity on fibroblasts against reactive oxygen species activity within the cell, demonstrating their fundamental role in the development and maturation of dogs in the early stages of their life.

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Section: Introductionmentioning

confidence: 99%

Canine colostrum exosomes: characterization and influence on the canine mesenchymal stem cell secretory profile and fibroblast anti-oxidative capacity

et al. 2020

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“…The regeneration of the intestinal epithelium, together with a reduction of the inflammatory response, led to increased survival in rats that underwent high-dose abdominal irradiation. Moreover, ADRCs were previously described to have a high potential for differentiation into connective tissue cells [11], which may have contributed to the successful closure of the fistula in our patient.…”

mentioning

confidence: 76%

Successful treatment of an enterovesical fistula due to Crohn’s disease with stem cell transplantation: a case report

Moniuszko

Sarnowska

Rogowski

et al. 2018

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“…The effect of CCM exosomes has been evaluated in some cell types [30]; however, it has never been assessed in mesenchymal stem cells (MSCs). MSCs play a strategic role in the development, homeostasis and repair of different organs and tissues [31,32] and have shown promising results in the treatment of different canine diseases [33,34]. Therefore, we believe that it is interesting to demonstrate the effect of canine colostrum exosomes on different types of MSCs to help understand their role in the neonatal period in the canine species.…”

Section: Introductionmentioning

confidence: 99%

Canine colostrum exosomes: Characterization and influence on the canine mesenchymal stem cell secretory profile and fibroblast anti-oxidative capacity.

Villatoro

Martín-Astorga

Alcoholado

et al. 2020

Preprint

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Background: Canine colostrum milk (CCM) is a specific secretion of the mammary gland that is fundamental for the survival of the newborn. CCM has many described components (immunoglobulins, proteins or fat), but its small vesicles, named exosomes, are largely unknown.Results: A characterization of CCM exosomes was performed. Exosomes were abundant in CCM and appeared with the characteristic cup-shaped morphology and well-defined round vesicles. The size distribution of exosomes was between 37 and 140 nm, and western blot analysis showed positive expression of specific exosomal markers. Proteomic analysis revealed a total of 826 proteins in exosome cargo. We also found that exosomes modified the proliferation and secretory profiles in canine mesenchymal stem cells derived from bone marrow (cBM-MSCs) and adipose tissue (cAd-MSCs). Additionally, CCM exosomes demonstrated a potent antioxidant effect on canine fibroblasts in culture.Conclusions: Our findings highlight, for the first time, the abundant presence of exosomes in CCM and their ability to interact with mesenchymal stem cells (MSCs). The addition of exosomes to two types of MSCs in culture resulted in specific secretory profiles with functions related to angiogenesis, migration and chemotaxis of immune cells. In particular, the cAd-MSCs secretory profile showed higher potential in adipose tissue development and neurogenesis, while cBM-MSC production was associated with immunity, cell mobilization and haematopoiesis. Finally, exosomes also presented antioxidant capacity on fibroblasts against reactive oxygen species activity within the cell, demonstrating their fundamental role in the development and maturation of dogs in the early stages of their life.

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Differentiation of canine adipose tissue–derived mesenchymal stem cells towards endothelial progenitor cells

Abstract: Results indicated that canine ASCs developed EPC characteristics when stimulated by differentiation medium with growth factors. Thus, differentiated canine ASC-EPCs may have the potential to provide vascularization for constructs used in regenerative medicine strategies.

Cited by 12 publications

References 27 publications

Canine colostrum exosomes: characterization and influence on the canine mesenchymal stem cell secretory profile and fibroblast anti-oxidative capacity

Canine colostrum exosomes: characterization and influence on the canine mesenchymal stem cell secretory profile and fibroblast anti-oxidative capacity

Successful treatment of an enterovesical fistula due to Crohn’s disease with stem cell transplantation: a case report

Canine colostrum exosomes: Characterization and influence on the canine mesenchymal stem cell secretory profile and fibroblast anti-oxidative capacity.

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