Differentiation of cardiac Purkinje fibers requires precise spatiotemporal regulation of Nkx2‐5 expression

Harris, Brett S.; Spruill, Laura; Edmonson, Angela M.; Rackley, Mary S.; Benson, D. Woodrow; O’Brien, Terrence X.; Gourdie, Robert G.

doi:10.1002/dvdy.20580

Cited by 36 publications

(27 citation statements)

References 44 publications

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“…It has been found that Nkx-2.5 plays critical roles in regulating tissue-specific gene expression and induces chicken cardiac myocyte differentiation in tissues (Schultheiss et al, 1995;Harris et al, 2006). Cyclic adenosine monophosphate response element-binding protein, which binds to the cyclic adenosine monophosphate response elements and increases or decreases the transcription of genes, is critical in a variety of cellular processes such as cell proliferation, differentiation, and adaptive responses in chickens (Sirotkin and Grossmann, 2003;Kim et al, 2005).…”

Section: Association Of Growth-correlated Genes With Economic Traitsmentioning

confidence: 98%

Polymorphisms of three neuroendocrine-correlated genes associated with growth and reproductive traits in the chicken

Tang

Sun

et al. 2009

Poultry Science

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The identification and utilization of potential candidate genes for QTL with significant effects on economically important traits are becoming increasingly important in poultry breeding programs. Chicken insulin-like growth factor binding protein 1 and 3 and signal transducers and activators of transcription 5B (STAT5B) genes are 3 essential nodes for signaling pathways and gene networks of growth and reproduction. The pooled DNA sequencing approach was used for identification of 9 SNP of the 5' upstream region of the 3 genes. A total of 826 individuals from Beijing You chicken were genotyped for 5 SNP using a modified PCR-RFLP method and the association with chicken growth and reproductive traits was studied using the GLM procedure. The T56039403C (T-808C) SNP of the insulin-like growth factor binding protein 1 gene was associated with BW at 10 wk of age (P = 0.0061), and the C56072547T (C-968T) SNP of the insulin-like growth factor binding protein 3 gene was associated with BW at 8 and 10 wk of age (P = 0.0056 and P = 0.0016, respectively). The C4535156T (C-1591T), G4533815A (G-250A), and G4533675C (G-110C) SNP of the STAT5B gene were associated with age at first egg (P = 0.0143, P = 0.0088, and P = 0.0114, respectively). Moreover, Lewontin's D' (|D'|) and r(2) of C4535156T and G4533815A SNP, C4535156T and G4533675C SNP, and G4533815A and G4533675C SNP of the STAT5B gene were 0.939 and 0.852, 0.967 and 0.858, and 0.971 and 0.896, respectively. The 3 SNP were strong-linked with each other and lay within a haplotype block. Our results suggest that these SNP were significantly associated with early growth or with sexual maturation in chickens, or both, and may be potential molecular markers for MAS.

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Section: Association Of Growth-correlated Genes With Economic Traitsmentioning

confidence: 98%

Polymorphisms of three neuroendocrine-correlated genes associated with growth and reproductive traits in the chicken

Tang

Sun

et al. 2009

Poultry Science

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“…In addition, investigators have also demonstrated a role for Nkx.2.5 in specification of the peripheral conduction system in a time- and dose-dependent manner 26, 27 . Nkx2.5 haploinsufficiency causes hypocellularity of the peripheral conduction system.…”

Section: Homeobox Proteinsmentioning

confidence: 99%

Specification of the Cardiac Conduction System by Transcription Factors

Hatcher

Basson

2009

Circulation Research

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Diseases of the cardiovascular system that cause sudden cardiac deaths are often caused by lethal arrhythmias that originate from defects in the cardiac conduction system. Development of the cardiac conduction system is a complex biological process that can be wrought with problems. Although several genes involved in mature conduction system function have been identified, their association with development of specific subcomponents of the cardiac conduction system remains challenging. Several transcription factors, including homeodomain proteins and T-box proteins, are essential for cardiac conduction system morphogenesis and activation or repression of key regulatory genes. In addition, several transcription factors modify expression of genes encoding the ion channel proteins that contribute to the electrophysiological properties of the conduction system and govern contraction of the surrounding myocardium. Loss of transcriptional regulation during cardiac development has detrimental effects on cardiogenesis that may lead to arrhythmias. Human genetic mutations in some of these transcription factors have been identified and are known to cause congenital heart diseases that include cardiac conduction system malformations. In this review, we summarize the contributions of several key transcription factors to specification, patterning, maturation, and function of the cardiac conduction system. Further analysis of the molecular programs involved in this process should lead to improved diagnosis and therapy of conduction system disease. (Circ Res. 2009;105:620-630.)

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“…In addition, there was a severely limited range of interrogation angles for Doppler imaging due to the egg shell. These limitations were largely overcome by using an ex ovo culture setup (Dunn et al, 1981;Tuan, 1983;Ono and Tuan, 1986;Harris et al, 2006), which allowed much freer positioning of the scanhead (Fig. 1) and additionally re- Using an ex ovo culture setup, unhindered access is obtained, and multiple scanning interrogation angles are possible.…”

Section: Optimization Of Imaging Conditionsmentioning

confidence: 99%

High‐frequency ultrasonographic imaging of avian cardiovascular development

McQuinn

Bratoeva

deAlmeida

et al. 2007

Developmental Dynamics

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The chick embryo has long been a favorite model system for morphologic and physiologic studies of the developing heart, largely because of its easy visualization and amenability to experimental manipulations. However, this advantage is diminished after 5 days of incubation, when rapidly growing chorioallantoic membranes reduce visibility of the embryo. Using high-frequency ultrasound, we show that chick embryonic cardiovascular structures can be readily visualized throughout the period of Stages 9 -39. At most stages of development, a simple ex ovo culture technique provided the best imaging opportunities. We have measured cardiac and vascular structures, blood flow velocities, and calculated ventricular volumes as early as Stage 11 with values comparable to those previously obtained using video microscopy. The endocardial and myocardial layers of the pre-septated heart are readily seen as well as the acellular layer of the cardiac jelly. Ventricular inflow in the pre-septated heart is biphasic, just as in the mature heart, and is converted to a monophasic

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Differentiation of cardiac Purkinje fibers requires precise spatiotemporal regulation of Nkx2‐5 expression

Cited by 36 publications

References 44 publications

Polymorphisms of three neuroendocrine-correlated genes associated with growth and reproductive traits in the chicken

Polymorphisms of three neuroendocrine-correlated genes associated with growth and reproductive traits in the chicken

Specification of the Cardiac Conduction System by Transcription Factors

High‐frequency ultrasonographic imaging of avian cardiovascular development

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