Differentiation of Cronobacter spp. by tryptic digestion of the cell suspension followed by MALDI-TOF MS analysis

Krásný, Lukáš; Rohlová, Eva; Růžičková, H.; Šantrůček, Jiří; Hynek, Radovan; Hochel, Igor

doi:10.1016/j.mimet.2014.01.008

Cited by 12 publications

(3 citation statements)

References 27 publications

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“…Since then, over 200 papers have been published per year for bacteria identification using the mass spectrometer. Advances in mass spectrometry techniques and instrument development have resulted in improved detection of bacteria species; however, most studies utilize matrix-assisted laser desorption/ionization—time of flight mass spectrometry (MALDI-TOF) [9,10,11,12,13,14,15,16,17,18,19]. Recently, in 2013 the U.S. Food and Drug Administration (FDA) approved two matrix-assisted laser desorption-time of flight-mass spectrometry (MALDI-TOF-MS)-based platforms for bacteria identification [20].…”

Section: Introductionmentioning

confidence: 99%

Identification of Pathogenic Bacteria from Public Libraries via Proteomics Analysis

Jung

Kim

Bhatt

et al. 2019

IJERPH

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Hazardous organisms may thrive on surfaces that are often exposed to human contact, including children’s library books. In this study, swab samples were taken from 42 children’s books collected from four public libraries in Texas and California. Samples were then cultivated in brain–heart infusion (BHI) medium and then in Luria broth (LB) medium containing either ampicillin or kanamycin. All 42 samples (100%) were positive for bacterial growth in normal BHI medium. Furthermore, 35 samples (83.3%) and 20 samples (47.6%) in total were positive in LB medium containing ampicillin or kanamycin, respectively. Bacterial populations were then identified in samples using an Orbitrap Fusion™ Tribrid ™ mass spectrometer, a state-of-the-art proteomic analysis tool. Identified bacterial species grown in ampicillin included Bacillus, Acinetobacter, Pseudomonas, Staphylococcus, Enterobacter, Klebsiella, Serratia, Streptococcus, Escherichia, Salmonella, and Enterococcus. In contrast, identified bacteria grown in kanamycin included Staphylococcus, Streptococcus, Enterococcus, and Bacillus. The presences of pathogenic bacteria species were also confirmed. The results of this study warrant follow up studies to assess the potential health risks of identified pathogens. This study demonstrates the utility of proteomics in identifying environmental pathogenic bacteria for specific public health risk evaluations.

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Section: Introductionmentioning

confidence: 99%

Identification of Pathogenic Bacteria from Public Libraries via Proteomics Analysis

Jung

Kim

Bhatt

et al. 2019

IJERPH

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“…LC‐ESI‐QTOF analysis was carried out using a UHPLC Dionex Ultimate3000 RSLC nano (Dionex, Germany) connected with Maxis Impact (Bruker, Germany) mass spectrometer as described in detail in Krásný, et al Peak lists were extracted from raw data by Data Analysis (Bruker Daltonics, Germany). Proteins were identified using Mascot version 2.2.04 (Matrix Science, UK) by searching NCBInr protein database version 20120319.…”

Section: Methodsmentioning

confidence: 99%

Separation and identification of candidate protein elicitors from the cultivation medium of Leptosphaeria maculans inducing resistance in Brassica napus

Nováková

Kim

Šašek

et al. 2016

Biotechnology Progress

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The Dothideomycete Leptosphaeria maculans, a worldwide fungal pathogen of oilseed rape (Brassica napus), secretes a broad spectrum of molecules into the cultivation medium during growth in vitro. Here, candidate elicitor molecules, which induce resistance in B. napus to L. maculans, were identified in the cultivation medium. The elicitation activity was indicated by increased transcription of pathogenesis-related gene 1 (PR1) and enhanced resistance of B. napus plants to the invasion of L. maculans. The elicitation activity was significantly lowered when the cultivation medium was heated to 80°C. Active components were further characterized by specific cleavage with the proteolytic enzymes trypsin and proteinase K and with glycosidases α-amylase and β-glucanase. The elicitor activity was eliminated by proteolytic digestion while glycosidases had no effect. The filtered medium was fractionated by either ion-exchange chromatography or isoelectric focusing. Mass spectrometry analysis of the most active fractions obtained by both separation procedures revealed predominantly enzymes that can be involved in the degradation of plant cell wall polysaccharides. This is the first study searching for L. maculans-specific secreted elicitors with a potential to be used as defense-activating agents in the protection of B. napus against L. maculans in agriculture. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:918-928, 2016.

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“…Cronobacter spp. has been isolated from corn and corn products such as corn starch, corn grits, and corn 1302 (2024) 012101 IOP Publishing doi:10.1088/1755-1315/1302/1/012101 2 flour [9,10,11,12]. In Indonesia, one positive corn starch sample contained two isolates of Cronobacter spp.…”

Section: Introductionmentioning

confidence: 99%

Application of pGFPuv mutant to study Cronobacter sakazakii survival in corn flour during storage

Sinamo,

Dewanti-Hariyadi,

Suliantari

2024

IOP Conf. Ser.: Earth Environ. Sci.

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Cronobacter sakazakii is more resistant in dry conditions for more than 2 years. However, study on the behavior of C. sakazakii in dry product using wild type may encounter problem of inability to distinguish target C. sakazakii with naturally occuring C. sakazakii. This research is useful in studying of C.sakazakii pFGPuv in corn flour having different initial moisture content stored in different relative humidity to ensure food safety and sustainability. The corn flour had a water content of 9% and 12 (w.b.) which had been inoculated with C. sakazakii pFGPuv for 16 weeks at 3 different RHs (50%, 70%, and 90%). The survival ability of C. sakazakii of GFPuv was better in corn flour with an Aw of 0.42-0.44 stored at RH 50% compared to RH 70 and 90%. C. sakazakii pGFPuv survived better at RH 50% which had Aw of 0.42-0.44 which was stored at RH 50% compared to RH 70 and 90%. The number of C. sakazakii pGFPuv in corn flour decreased by 0.470-0.489 log cycle/week. Therefore, the number of C. sakazakii in corn flour that will be stored needs to be considered to produce food that is safe to support good health and well-being.

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Differentiation of Cronobacter spp. by tryptic digestion of the cell suspension followed by MALDI-TOF MS analysis

Cited by 12 publications

References 27 publications

Identification of Pathogenic Bacteria from Public Libraries via Proteomics Analysis

Identification of Pathogenic Bacteria from Public Libraries via Proteomics Analysis

Separation and identification of candidate protein elicitors from the cultivation medium of Leptosphaeria maculans inducing resistance in Brassica napus

Application of pGFPuv mutant to study Cronobacter sakazakii survival in corn flour during storage

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