Differentiation of Dental Pulp Stem Cells on Gutta-Percha Scaffolds

Zhang, Liudi; Yu, Yingjie; Joubert, Christopher; Bruder, George A.; Liu, Ying; Chang, Chung-Chueh; Simon, Marcia; Walker, Stephen G.; Rafailovich, Miriam

doi:10.3390/polym8050193

Cited by 21 publications

(16 citation statements)

References 29 publications

(31 reference statements)

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“…The recommendations suggest that pure compounds with cytotoxic IC 50 values B 4 lg/ml are considered active (Alonso-Castro et al 2011) more important when considering that the fractions are not pure substances but mixtures of compounds. The increase of cytotoxicity when the gum was removed from the extract may have a relationship with previous reports of trans-1,4-polyisoprene (the main component of the gutta-percha) as an inductor of cell growth and differentiation (Zhang et al 2016). Polyisoprenes can participate as initial substrates of the mevalonate pathway and promote cell proliferation, particularly in tumor cells (Mullen et al 2016).…”

Section: Fractionation Of the Petroleum Ether Extract Of S Mexicanumsupporting

confidence: 76%

Cytotoxic effect of Semialarium mexicanum (Miers) Mennega root bark extracts and fractions against breast cancer cells

Maldonado-Cubas

Martín‐Martínez

Quiroz-Reyes

et al. 2018

Physiol Mol Biol Plants

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The root bark of Semialarium mexicanum (Miers) Mennega (cancerina) is traditionally used in Mexico to treat cancer. However, there are no studies supporting its use. We evaluated whether S. mexicanum root bark induces cytotoxicity in breast cancer cells to determine if it has potential applications in the treatment of this disease. Extracts of S. mexicanum root bark in petroleum ether, ethanol, and water were obtained by ultrasound-assisted extraction. MTT and WST-1 assays were used to evaluate the cytotoxicity of the extracts toward breast cancer cells (MDA-MB-231 and MCF7), non-tumorigenic breast-derived cells (MCF 10A), and peripheral blood mononuclear cells (PBMCs). For the extract with greatest cytotoxicity, induction of apoptosis and oxidative stress were determined using flow cytometry. The extract was fractionated, and the cytotoxicity of its fractions was evaluated with the four cell types. The fractions were also analyzed by HPLC. Only the petroleum ether extract was cytotoxic for all cell types (MDA-MB-231 [ MCF 10A/ MCF7 [ PBMCs). Cell death occurred by apoptosis, which could be associated with the induction of oxidative stress. Two fractions that were highly cytotoxic for breast cancer cells were obtained from this extract (IC 50-B 4.15 lg/mL for the most active fraction at 72 h). The MCF 10A cells were less affected, while PBMCs were not affected after 72 h of treatment. Pristimerin was identified in both fractions and may be partially responsible for the cytotoxic effect. These results suggest that S. mexicanum root bark has a potential application in breast cancer treatment.

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Section: Fractionation Of the Petroleum Ether Extract Of S Mexicanumsupporting

confidence: 76%

Cytotoxic effect of Semialarium mexicanum (Miers) Mennega root bark extracts and fractions against breast cancer cells

Maldonado-Cubas

Martín‐Martínez

Quiroz-Reyes

et al. 2018

Physiol Mol Biol Plants

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“…The complicated molecular structures of polysaccharides partly contribute to its multiple biological functions [19]. Sulfur-containing groups, such as sulfonate and sulfate, can be part of some natural polysaccharides and provide the polysaccharides more diversity [20,21]. The sulfated polysaccharides are also called fucoidan from SF, which shows antioxidation, anti-inflammation, antitumor, antiradiation, and anticoagulation effects.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Polysaccharides Extracted from Sargassum fusiforme and Its Effective Prevention of Contrast-Induced Nephropathy via Enhancing Antioxidant Capacity

Dai

Zhou

Jiang

et al. 2019

International Journal of Polymer Science

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Contrast-induced nephropathy (CIN) is a common complication in patients with coronary arteriography, and oxidative stress is involved in the CIN pathogenesis. Sargassum fusiforme (SF) is a brown seaweed with medicinal value, and its polysaccharides have good antioxidant activity. In this study, the crude polysaccharides (cSFP-C) were extracted by cold water, precipitated by ethanol, purified by CaCl2, and detected with high contents of sulfate radical and fucose. cSFP-C is composed of glucose, glucuronic acid, xylose, rhamnose, mannose, galactose, and fucose with a molar ratio of 1.0 : 0.4 : 5.6 : 1.2 : 1.7 : 12.3 : 56.1. The cSFP-C has the typical absorption of polysaccharides. Antioxidation assays in vitro showed that cSFP-C exhibited superoxide radical scavenging activity which was better than the hot water-extracted crude polysaccharides (cSFP-H). 20 rats were divided into 4 groups (n=5): sham group; CIN group; CIN+cSFP-C group, and cSFP-C group. The CIN+cSFP-C group and cSFP-C group were pretreated intragastrically with cSFP-C at a dose of 9.45 g/kg twice daily for 5 consecutive days. Then, the CIN group and CIN+cSFP-C group were given indomethacin to develop CIN. The in vivo results showed that cSFP-C could decrease blood creatinine and urea nitrogen, inhibiting pathological injury in the renal tissues. The MDA content of renal tissues was decreased, while the activity of SOD was increased. The crude sulfated polysaccharides extracted from S. fusiforme have a renoprotective effect on oxidative stress to alleviate the kidney injury in CIN rats.

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“…It is probable that in addition to obturation, GP nanocomposites can act as scaffolds for dental tissue regeneration. [28]…”

Section: Biological Properties and Tissue Interactionmentioning

confidence: 99%

Gutta-percha in endodontics - A comprehensive review of material science

Vishwanath

Rao

2019

J Conserv Dent

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The complete and three-dimensional fluid tight seal of the root canal system is the final component of the endodontic triad. The long-standing and closest material which has fulfilled this criterion is gutta-percha (GP). Several materials have been tried and tested as an endodontic filling material, of which GP has been most extensively used for years and has established itself as a gold standard. In addition, it has proved itself successful with different techniques of obturation while maintaining its basic requisites. This article deals briefly with the history and evolution of GP, source, chemical composition, manufacturing, disinfection, cross-reactivity, and advancements in the material.

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Differentiation of Dental Pulp Stem Cells on Gutta-Percha Scaffolds

Cited by 21 publications

References 29 publications

Cytotoxic effect of Semialarium mexicanum (Miers) Mennega root bark extracts and fractions against breast cancer cells

Cytotoxic effect of Semialarium mexicanum (Miers) Mennega root bark extracts and fractions against breast cancer cells

Characterization of Polysaccharides Extracted from Sargassum fusiforme and Its Effective Prevention of Contrast-Induced Nephropathy via Enhancing Antioxidant Capacity

Gutta-percha in endodontics - A comprehensive review of material science

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