Differentiation of Drosophila neuroblasts to form ganglion-like clusters of neurons in vitro

Seecof, Robert L.; Donady, J. James; Teplitz, Raymond L.

doi:10.1016/0045-6039(73)90014-6

Cited by 68 publications

(20 citation statements)

References 3 publications

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“…Sa-d. The nerve cell clusters presumably arose from these angular cells by division as already suggested by Seecof et al (1973). Generally, the number of nerve cells within the aggregates is consistent with them having arisen by a series of eight budding divisions followed by an equational division as described by Seecof et al (1973).…”

Section: In Vitro Cell Culture Of Fragmented Gastrulae (I) Characterimentioning

confidence: 49%

Characterization of a Temperature-Sensitive Female Sterile Mutant (l(1)1074TS) in Drosophila Melanogaster

Datson¹,

Brink²

1978

Aust. Jnl. Of Bio. Sci.

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A new X-linked temperature-sensitive female sterile mutant (/(1)1074") is described. The nonpermissive temperature for this mutant is 29°C. There are two temperature-sensitive periods during development-one between the 6th and 12th hours of embryogenesis and a second commencing during the first larval instar and terminating at mid pupation. Embryological abnormalities first become apparent during gastrulation and eventually these result in the breakdown of organogenesis and the complete absence of normal muscular contractions. Preconditioning mutant females at the nonpermissive temperature for up to 48 h enhances the abnormal embryological effects produced by the mutant.When developing gastrulae of the mutant are fragmented and cultured in vitro at 29°C, many cell clumps fail to differentiate. This does not appear to be a consequence of cell death. Fragmented gastrulae of wild-type embryos always show cell differentiation following in vitro culture.On the basis of the studies reported in this paper, it is suggested that the /(1)1074+ gene product is deposited within the ovum during oogenesis and is vital for a number of early embryonic developmental processes rather than being critical for any specific developmental event such· as the differentiation of a particular cell type.

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Section: In Vitro Cell Culture Of Fragmented Gastrulae (I) Characterimentioning

confidence: 49%

Characterization of a Temperature-Sensitive Female Sterile Mutant (l(1)1074TS) in Drosophila Melanogaster

Datson¹,

Brink²

1978

Aust. Jnl. Of Bio. Sci.

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“…Gastrula cells in culture have been observed to differentiate into distinct cell types including neurons, myocytes, fused myotubes, fibroblasts, and macrophage-like cells (Shields and Sang, 1970;Seecof, Teplitz, Gerson, et al, 197 1 ;Chan and Gehring, 197 1 ) . Neuroblasts from these cultures were observed to divide asymmetrically, extend neurites, and form neuromuscular junctions with adjacent myocytes, just as they do in vivo (Seecof et al, 1971(Seecof et al, , 1972(Seecof et al, , 1973. Further, the spatial and temporal appearance in culture of the neurotransmitters acetylcholine, serotonin, and dopamine, and their associated enzymes, appear to mimic that observed in vivo ( Dewhurst and Seecof, 1975;Furst and Mahowald, 1985;White and Valles, 1985;Scholnick, Bray, Morgan, et al, 1986;Huff et al, 1989).…”

Section: The In Vitro Approachmentioning

confidence: 71%

Approaches to identify genes involved in Drosophila embryonic CNS development

Noll

Perkins²,

Mahowald

et al. 1993

J. Neurobiol.

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Many of the steps involved in formation of the Drosophila embryonic central nervous system (CNS) have been identified by both descriptive and experimental studies. In this review we will describe the various approaches that have been used to identify molecules involved in CNS development and the advantages and disadvantages of each of them. Our discussion will by no means be exhaustive; but rather we will discuss our experiences with each approach and provide an overview of what has been learned by using these methodologies. Finally, we will discuss methods that have been recently developed and how they are likely to provide further insight into CNS development.

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“…Neuromeres (Seecof et al 1973; Fredieu and Mahowald 1989) are clusters of 6–20 neural cell bodies associated with glial cells. It is essential to segment out neuromeres prior to neurite tracing because their complicated texture can compromise neurite extraction efforts and lead to false or erroneous detection of neurites.…”

Section: Methodsmentioning

confidence: 99%

Automatic Robust Neurite Detection and Morphological Analysis of Neuronal Cell Cultures in High-content Screening

Schulte

Sepp

et al. 2010

Neuroinform

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Cell-based high content screening (HCS) is becoming an important and increasingly favored approach in therapeutic drug discovery and functional genomics. In HCS, changes in cellular morphology and biomarker distributions provide an information-rich profile of cellular responses to experimental treatments such as small molecules or gene knockdown probes. One obstacle that currently exists with such cell-based assays is the availability of image processing algorithms that are capable of reliably and automatically analyzing large HCS image sets. HCS images of primary neuronal cell cultures are particularly challenging to analyze due to complex cellular morphology. Here we present a robust method for quantifying and statistically analyzing the morphology of neuronal cells in HCS images. The major advantages of our method over existing software lie in its capability to correct non-uniform illumination using the contrast-limited adaptive histogram equalization method; segment neuromeres using Gabor-wavelet texture analysis; and detect faint neurites by a novel phase-based neurite extraction algorithm that is invariant to changes in illumination and contrast and can accurately localize neurites. Our method was successfully applied to analyze a large HCS image set generated in a morphology screen for polyglutaminemediated neuronal toxicity using primary neuronal cell cultures derived from embryos of a Drosophila Huntington's Disease (HD) model. © Springer Science+Business Media, LLC 2010Correspondence to: Pengyu Hong, hongpeng@brandeis.edu. NIH Public Access Author ManuscriptNeuroinformatics. Author manuscript; available in PMC 2011 January 18. has been adopted to conduct analysis of primary neurons which have highly complex cellular morphologies and branching patterns. This type of analysis has been a powerful methodology in RNA interference (RNAi) screening experiments to identify genes that are important for neuronal development, physiology and disease (Dragunow 2008;Sepp et al. 2008). Such studies are expected to be an increasingly utilized approach in drug discovery as neuronal morphology can be used as an assay for cell health.Noise and complex signals make it challenging to quantify neurites and neuromeres, which are two essential morphological elements that relate to neuronal health status. For example, noise can arise from non-neuronal cells, cell debris, or limitations in the sensitivity of charge-coupled device (CCD) cameras that are commonly used to collect images. The signal levels of key neurites can be very weak because of various factors, including variations in the thickness of the neurites, sub-optimal focus plane established by automated microscopy, 3-dimensional nature of the cell cultures, and inadequate exposure time lengths. In general, microscope settings for a screen are established on the basis of what is optimal for an average well of a multiwell screening plate. However, cell profiles in some wells may be significantly different in marker intensities, which can lead to under-or over-e...

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Differentiation of Drosophila neuroblasts to form ganglion-like clusters of neurons in vitro

Cited by 68 publications

References 3 publications

Characterization of a Temperature-Sensitive Female Sterile Mutant (l(1)1074TS) in Drosophila Melanogaster

Characterization of a Temperature-Sensitive Female Sterile Mutant (l(1)1074TS) in Drosophila Melanogaster

Approaches to identify genes involved in Drosophila embryonic CNS development

Automatic Robust Neurite Detection and Morphological Analysis of Neuronal Cell Cultures in High-content Screening

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