Differentiation of ex vivo human breast tissue using polarization-sensitive optical coherence tomography

Abstract

Successful treatment of breast cancer typically requires surgical removal of the tumor. Optical coherence tomography (OCT) has been previously developed for real-time imaging of the surgical margin. However, it can be difficult to distinguish between normal stromal tissue and cancer tissue based on scattering intensity and structure alone. Polarization-sensitive optical coherence tomography (PS-OCT) is sensitive to form birefringence of biological tissue. We report on the development of a high-speed PS-OCT system and imaging of ex vivo human breast tissue, showing enhanced contrast between healthy and cancerous tissues based upon collagen content confirmed with corresponding histology. These results demonstrate the feasibility of using PS-OCT to supplement structural OCT as a possible method for intraoperative tumor margin evaluation. References and links 1. American Cancer Society, Breast Cancer Facts & Figs. 2013 (American Cancer Society, Atlanta, GA, 2013. 2. B. Fisher, S. Anderson, J. Bryant, R. G. Margolese, M. Deutsch, E. R. Fisher, J.-H. Jeong, and N. Wolmark, "Twenty-year follow-up of a randomized trial comparing total mastectomy, lumpectomy, and lumpectomy plus irradiation for the treatment of invasive breast cancer," N. Engl. J. Med. 347(16), 1233-1241 (2002). 3. U. Veronesi, N. Cascinelli, L. Mariani, M. Greco, R. Saccozzi, A. Luini, M. Aguilar, and E. Marubini, "Twentyyear follow-up of a randomized study comparing breast-conserving surgery with radical mastectomy for early breast cancer," N. Engl. J. Med. 347(16), 1227-1232 (2002). 4. P. Ananthakrishnan, F. L. Balci, and J. P. Crowe, "Optimizing surgical margins in breast conservation," Int. J. Surg. Oncol. 2012, 585670 (2012 J. Keely, "Aligned collagen is a prognostic signature for survival in human breast carcinoma," Am. ©2014 Optical Society of America

An Engineered Breast Cancer Model on a Chip to Replicate ECM‐Activation In Vitro during Tumor Progression

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Differentiation of Different Nonmelanoma Skin Cancer Types Using OCT

“…For instance, OCT is already used in gastroenterology to detect malignant lesions in the gastrointestinal tract as well as possible responses to the resulting cancer therapy [9-11]. Moreover, application possibilities of OCT regarding breast cancer have been investigated in gynecology [12, 13]. The application of OCT in the field of neurology is also highly interesting.…”

Polarization Sensitive Optical Coherence Tomography: A Review of Technology and Applications

“…Further promising results of PS-OCT based cancer imaging were reported in larynx, ovaries, and bladder [32,184,185]. Several groups also successfully studied PS-OCT for imaging breast cancer [112,186,187]. Figure 8 shows exciting results of PS-OCT imaging, which enabled the differentiation of tumor from surrounding tissue.…”

“…Several groups also successfully studied PS-OCT for imaging breast cancer [112,186,187]. Figure 8 shows exciting results of PS-OCT imaging, which enabled the differentiation of tumor from surrounding tissue.…”