Differentiation of five tuna species by a multiplex primer-extension assay

Bottero, Maria Teresa; Dalmasso, Alessandra; Cappelletti, Marco; Secchi, C.; Civera, Tiziana

doi:10.1016/j.jbiotec.2007.01.032

Cited by 48 publications

(30 citation statements)

References 16 publications

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“…[7,8] Nevertheless, fragment size is a limiting factor for the subsequent PCR reaction, which is based on the selective amplification of specific regions of DNA using oligonucleotides. [8] PCR [9][10][11] and its modifications-polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), [12][13][14][15] Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP), [16,17] real-time PCR, [18][19][20][21] and Polymerase chain reaction-enzyme-linked immunosorbent assay (PCR-ELISA) [22] -represent crucial approaches for tuna fish species identification. These approaches comprise DNA extraction from the sample, PCR, and electrophoresis, or alternatively other detection systems for the evaluation of the final results.…”

Section: Introductionmentioning

confidence: 99%

Comparative study of DNA extraction methods from fresh and processed yellowfin tuna muscle tissue

Piskatá

Pospisilova

Bořilová

2017

International Journal of Food Properties

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The most common techniques used to identify tuna species include methods based on the detection of specific DNA. The quality of species-specific DNA crucially affects the efficiency of amplification during the subsequent polymerase chain reaction (PCR). Detection of DNA in processed products can be adversely affected by DNA fragmentation during the processing steps and the use of ingredients that may inhibit the PCR reaction. In this study, several processing treatments applied to the muscle tissue of yellowfin tuna (Thunnus albacares) were evaluated. For DNA isolation three DNA extraction methods were compared. The concentration, purity, and amplificability of DNA were tested. The results revealed the variability among extraction procedures in terms of DNA quality and quantity in tuna muscle tissue processed under different processing technologies. ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

Comparative study of DNA extraction methods from fresh and processed yellowfin tuna muscle tissue

Piskatá

Pospisilova

Bořilová

2017

International Journal of Food Properties

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“…Recently Bottero, Dalmasso, Cappelletti, Secchi, and Civera (2007) successfully applied a method based on minisequencing reaction to differentiate closely related species of tuna fish in canned products. This technique consists in the analysis of the diagnosis sites present in a fragment previously amplified and is based on the dideoxy (ddNTP) single base extension of an unlabelled oligonucleotide (sequencing primer) at the 3 0 end of the base immediately adjacent to the diagnosis site.…”

Section: Introductionmentioning

confidence: 99%

Authentication of meat from game and domestic species by SNaPshot minisequencing analysis

Neve

Civera

Mucci³

et al. 2008

Meat Science

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The aim of the present study is to develop an assay for the specific identification of meat from Capreolus capreolus, Cervus elaphus, Capra ibex, Rupicapra rupicapra, targeting sequences of the cytochrome b (cyt b) gene of mitochondrial DNA. The assay is also intended to enable differentiation between meat from these wild species as well as Ovis aries, Capra hircus, Bubalus bubalis, Bos taurus and Sus scrofa domestic species.The primers used in the preliminary PCR were designed in well conserved regions upstream and downstream of the diagnosis sites. They successfully amplified a conserved 232 bp region from the cyt b gene of all the species taken into consideration. The sites of diagnosis have been interrogated using a minisequencing reaction and capillary electrophoresis. All the results of the multiplex PER (primer extension reaction) test were confirmed by fragment sequencing. The assay offers the possibility of discriminating nine species at the same time.

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“…Multiplex primer extension reaction has been successfully applied in discriminating many animal and bacterial species in different food items [28][29][30][31]. However, some problems could arise when mixed matrices are considered.…”

Section: Resultsmentioning

confidence: 99%

“…The small size of the amplified fragment (102 bp) allows application of the test to heat-treated products. In fact, thermal processing such as UHT procedure or sterilisation breaks the DNA in fragment of about 300 bp long [28].…”

Section: Rn Allele Y (Donkey)mentioning

confidence: 99%

Development of a real-time PCR assay for the detection of cow and donkey milk

Dalmasso

Sacchi

Bottero

2012

Eur Food Res Technol

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A real-time PCR allelic discrimination TaqMan assay based on the analysis of a single nucleotide polymorphism enabling the differentiation of cow (Bos taurus) and donkey (Equus asinus) milk was developed. Specific primers and probes were designed on the mitochondrial cytochrome c oxidase subunit I gene. The primers were designed upstream and downstream the chosen diagnosis site in a conserved region. Two probes were designed to specifically hybridise to B. taurus and E. asinus sequences. The test allowed the discrimination of bovine and donkey DNA in all blood and pure milk samples giving an unambiguous result plot of rapid and easy interpretation. The detection threshold was 2 % of cow milk in donkey milk. The applicability of the method to matrices containing degraded DNA was demonstrated by analysing samples of raw donkey and cow milk autoclave-treated (121°C for 15 min). Finally, the assay when applied to milk samples collected from the retail trade has confirmed the species indicated in the label. Furthermore, the assay represents a potentially valuable diagnostic tool for species identification in dairy products for allergic people.

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Differentiation of five tuna species by a multiplex primer-extension assay

Cited by 48 publications

References 16 publications

Comparative study of DNA extraction methods from fresh and processed yellowfin tuna muscle tissue

Comparative study of DNA extraction methods from fresh and processed yellowfin tuna muscle tissue

Authentication of meat from game and domestic species by SNaPshot minisequencing analysis

Development of a real-time PCR assay for the detection of cow and donkey milk

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