Differentiation of Group a From Other Beta Hemolytic Streptococci With Bacitracin

Levinson, M. L.; Frank, Paul F.

doi:10.1128/jb.69.3.284-287.1955

Cited by 50 publications

(11 citation statements)

References 3 publications

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“…Specific grouping of streptococci by the Lancefield precipitin method, however, is a time-consuming technique that is not routinely performed on all strains isolated in a hospital laboratory (8). Streptococci showing beta-hemolysis and bacitracin sensitivity are usually reported as belonging to group A (13,14). Organisms belonging to group C are usually beta-hemolytic, can be sensitive to bacitracin, and may cause an elevated serum anti-streptolysin 0 titer after respiratory tract infection (2, 13).…”

Section: Discussionmentioning

confidence: 99%

Streptococcus zooepidemicus (group C) pneumonia in a human

Rose¹,

Allen²,

G³

1980

J Clin Microbiol

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Lancefield group C streptococcal pneumonia appeared in a previously healthy young adult. The patient apparently acquired the infection while caring for her sick horse, and experienced a gradual onset of the illness. There was rapid accumulation of pleural fluid and empyema requiring open drainage. Group C pneumonia cannot be distinguished from classic group A pneumonia on clinical grounds. Beta-hemolytic streptococci isolated from sputum, transtracheal aspirates, pleural fluid, or blood of patients with pneumonia should be grouped by the precipitin method of Lancefield or one of its more rapid modifications.

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Section: Discussionmentioning

confidence: 99%

Streptococcus zooepidemicus (group C) pneumonia in a human

Rose¹,

Allen²,

G³

1980

J Clin Microbiol

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“…Between each sample in a given series, about 350 ml of sterile distilled water were employed to rinse the filter holders. The seeded filter grids (grid side up) were layered on absorbent pads previously placed in plastic disposable Petri dishes and impregnated with 0.8 ml of a modified Todd-Hewitt broth (Levinson and Frank, 1955) which is used for the routine isolation and identification of beta-hemolytic streptococci. The Todd-Hewitt broth contained 0.01 per cent 2,3,5-triphenyltetrazolium chloride and 0.04 per cent sodium azide (Slanetary et al, 1955).…”

Section: -Materials and M\ethodsmentioning

confidence: 99%

Adaptation of the Membrane Filter for the Isolation of Beta Hemolytic Streptococci1

Johnson¹,

Braunstein²

1958

Applied Microbiology

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Biological Engineer, for suggesting and helping to finance this study; Drs. Hadleigh Marsh and .J. E. Catlin of the Veterinary Research Laboratory at Montana State College for providing milk samples; Dr. A. Jasmin of the Mlontana Livestock Sanitary Board for providing cultures; and Mr. R. T. Henson of Flexible Products Company for arranging with different manufacturers to submit plastics for testing. SUMMARY The effects of 23 plastics in pellet and powder formn on stock cultures of Mlicrococcus pyogenes var. aureuts and Escherichia coli, on freshly isolated strains of a micrococcus and a streptococcus from abnormal milk, and on the microflora of raw milk from an injured udder were studied. On the basis of quantitative bacteriological tests, there was no evidence that the plastics of types including polyethylene, polyvinyl chloride, acrylic plastic, nylon resin, tri-and tetrafluoroethylene resin, polystyrene, ethylcellulose and cellulose acetate butyrate were toxic to the microorganisms investigated. These results indicate that it should be feasible to fabricate a single service plastic bag for transporting aseptically collected milk samples to the laboratory without the plastics studied being detrimental to the organisms present.

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“…Group A beta-hemolytic streptococci have traditionally been identified by susceptibility to bacitracin (4) or by the capillary precipitin test after appropriate extraction (8).…”

mentioning

confidence: 99%

“…The test organisms consisted of 305 beta-hemolytic streptococcal isolates which were obtained from throat cultures seen in a local private pediatric practice and from a variety of patient specimens received in the Bacteriology Laboratory at North Carolina Baptist Hospital, Winston-Salem. The organisms consisted of 240 group A, 30 group B, 14 group C, 4 group F, and 17 group G isolates. No group D isolates were included in this study because beta-hemolytic isolates are rarely encountered in routine clinical practice and are usually morphologically distinct from other beta-hemolytic groups.…”

mentioning

confidence: 99%