Differentiation of growth patterns of early gastric carcinoma determined by cytophotometric0 DNA analysis

Inokuchi, Kiyoshi; Kodama, Yoshifumi; Sasaki, Osamu; Kamegawa, Takahisa; Okamura, Tomonori

doi:10.1002/1097-0142(19830315)51:6<1138::aid-cncr2820510627>3.0.co;2-e

Cited by 110 publications

(12 citation statements)

References 10 publications

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“…In contrast, the Pen-A type tumor is characterized by dominant elevated lesions and a relatively high incidence of liver metastasis. We have investigated DNA ploidy 21 and p53 abnormality 22 in these different growth types of early gastric carcinoma. The Super type has diploidy and normal p53, whereas the Pen-A type has aneuploidy and abnormal p53.…”

Section: Discussionmentioning

confidence: 99%

Intratumoral neovascularization and growth pattern in early gastric carcinoma

Tomoda

Maehara

Kakeji

et al. 1999

Cancer

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Section: Discussionmentioning

confidence: 99%

Intratumoral neovascularization and growth pattern in early gastric carcinoma

Tomoda

Maehara

Kakeji

et al. 1999

Cancer

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“…The biological characteristics of highly malignant tumours have been studied by DNA analyses [18,22], and estimation of DNA abnormalities by means of flow cytometry or cytofluorometry may substantially contribute to knowledge of the biological characteristics of gastric cancer. Takai et al [57] reported that aneuploid tumours had significantly higher levels of uPA content than diploid ones.…”

Section: Discussionmentioning

confidence: 99%

Prognostic relevance of urokinase-type plasminogen activator (uPA) and plasminogen activator inhibitors PAI-1 and PAI-2 in gastric cancer

Ito

Yonemura

Fujita

et al. 1996

Vichows Archiv A Pathol Anat

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Expression of urokinase-type plasminogen activator (uPA), plasminogen activator inhibitor-1 (PAI-1) and plasminogen activator inhibitor-2 (PAI-2) was evaluated in 125 surgically resected gastric cancers by immunohistochemical analysis. Tissue was stained immunohistochemically with a monoclonal antibody against human uPA and monoclonal antibodies against human PAI-1 and PAI-2. In addition, DNA ploidy patterns were determined by cytofluorometer after staining with propidium iodide. We found that 82 (66%) of the 125 gastric cancers expressed uPA as diffuse cytoplasmic staining, as intensely outlined luminal borders. PAI-1 expression was observed in 62 (50%) of 125 gastric cancer as a fine, diffuse and granular pattern in the cytoplasm. PAI-2 expression was observed in 65 (52%) of the 125 gastric cancers as a diffuse cytoplasmic staining. uPA-positive tumours showed a higher incidence of infiltration, lymph node metastasis and peritoneal dissemination that uPA-negative ones. Patients with uPA-positive tumours proved to have a significantly poorer prognosis than those with negative ones. PAI-1-negative tumours showed a higher incidence of liver metastasis and carried a poorer prognosis than PAI-1-positive ones. There was no significant correlation between uPA or PAI-1 expression and DNA ploidy patterns. Conversely, there was no significant relationship between PAI-2 expression and clinicopathological parameters and prognosis. According to the expression of uPA and PAI-1 status, groups of 19 uPA(-)/PAI-1(-), 44 uPA(+)/PAI-1(-), 23 uPA(-)/PAI-1(+) and 39 uPA(+)/PAI-1(+) were subdivided. Tumours with uPA(+)/PAI-1(-) had a significantly higher incidence of liver metastasis, lymph node metastasis and serosal invasion than the other groups of tumours. Patients with uPA(+)/PAI-1(-) tumours had a significantly poorer prognosis than those with uPA(-)/PAI-1(+) tumours. These results indicate that uPA expression is a useful biological prognostic indicator, and that uPA expression is a useful biological prognostic indicator, and that uPA and PAI-1 may play an important part in the tumour progression and metastasis in gastric cancer.

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“…Alternatively, subtle differences, difficult to detect, might exist between the biological characteristics of neoplastic cells. Quantitative pathology (Tosi et al, 1984(Tosi et al, , 1986(Tosi et al, , 1987, image processing analysis (Schipper et al, 1987) and interpretation of DNA histograms of tumor cells by means of static (Inokuchi et al, 1983;Korenaga et al, 1985;Kamegawa et al, 1986) and flow cytometry (Fallenius et al, 1987) are among the means employed in the attempt to reveal such differences. In particular, DNA histograms by flow cytometry of routine histologically processed tissue have been analyzed in order to detect aneuploid patterns, which seem to be correlated with poorer prognosis.…”

Section: Discussionmentioning

confidence: 99%

Flow cytometric analysis of DNA ploidy pattern from deparaffinized formalin‐fixed gastric cancer tissue

Tosi

Leoncini

Cintorino

et al. 1988

Intl Journal of Cancer

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Histologically processed tissue from gastric cancers has been analyzed by flow cytometry in an attempt to correlate DNA ploidy pattern and behavior of the tumor. Of the mucosal and submucosal cancers (so-called early, all stage I in the present series), 62.7% show a diploid DNA pattern and 37.3% show a single aneuploid pattern. Of the deeply infiltrating (beyond the submucosa) cancers (stage II and III), 52.1% are single aneuploid and 47.9% are multiploid. While stage-I patients are all alive at the end of the follow-up period (6 years), in stage II and III cases Cox's regression model shows that the hazard function depends on DNA pattern: survival is negatively influenced by multiploidy. On this basis, it may be assumed that the DNA pattern is a useful prognostic indicator of gastric cancer. As expected, in Cox's regression model an even more important negative correlation exists between survival and stage: single aneuploid cases in stage II have a better prognosis than those in stage III. Instead, no correlation is found between histological cancer subtype (Laurén and WHO classifications), grade and DNA pattern.

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Differentiation of growth patterns of early gastric carcinoma determined by cytophotometric0 DNA analysis

Cited by 110 publications

References 10 publications

Intratumoral neovascularization and growth pattern in early gastric carcinoma

Intratumoral neovascularization and growth pattern in early gastric carcinoma

Prognostic relevance of urokinase-type plasminogen activator (uPA) and plasminogen activator inhibitors PAI-1 and PAI-2 in gastric cancer

Flow cytometric analysis of DNA ploidy pattern from deparaffinized formalin‐fixed gastric cancer tissue

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