Differentiation of herbs linked to “Chinese herb nephropathy” from the liquid chromatographic determination of aristolochic acids

Aristolochic Acids in the Roots of Aristolochia Chilensis, a Dangerous Chilean Medicinal Plant

“…[14][15][16][17][18][19][20][21][22][23][24] Their UV spectra ( Figure Nº 1) and retention times were compared, and co-injection was performed using standards of AA-I (1), AA-II (2), AA-III (3), AA-IV (4), AA-Ia (5), AA-IIIa (6), and AA-IVa (7), which had been previously isolated from A. chilensis, A. argentina, and Battus polydamas archidamas 22,25,26 . Quantification was based on peak areas of chromatograms taken at 254 nm.…”

“…In this study, high-performance liquid chromatography with diodearray detection (HPLC-DAD), a technique widely used for the detection and quantification of AAs in herbal medicine [14][15][16][17][18][19][20][21][22][23][24] was used to determine the chemical composition of AAs from the roots of A. chilensis. The findings reveal that the roots contain a significant amount of AAs, making it dangerous to use A. chilensis as a medicinal plant because of the harmful effects of AAs.…”

Assessment of Chinese medicinal herb metabolite profiles by UPLC‐MS‐based methodology for the detection of aristolochic acids

“…1), are constituents of the Aristolochia species of plant [4]. Aristolochia plants are commonly used in Chinese herbal medicines to treat tumours, snake bites, obstetrics, rheumatism, small pox and pneumonia [5]. However, the administration of medicines containing AA has been reported to cause renal disease [6,7].…”

“…In the past year, several publications for the detection or determination of AA have been released which include techniques using HPLC-UV [5,12], and CE [13][14][15]. Many of these methods are time consuming in sample preparation and in the runtime of each sample.…”

UHPLC Separation of Structurally Diverse Markers in Fangchi Species

“…Fangchi has been studied using several analytical methods, including HPLC-DAD, 5,6 LC-DAD-mass spectrometry (MS), 7,8 LC-DAD-MS/MS, 9 gas chromatography-MS (GC-MS), 10 capillary electrophoresis (CE). 11 Among them, HPLC methods have been popularly applied as an incisive tool for the quality control of herbal medicines.…”