Differentiation of human adipose-derived stem cells seeded on mineralized electrospun co-axial poly(ε-caprolactone) (PCL)/gelatin nanofibers

Abstract

Mineralized poly(ε-caprolactone)/gelatin core-shell nanofibers were prepared via co-axial electrospinning and subsequent incubation in biomimetic simulated body fluid containing ten times the calcium and phosphate ion concentrations found in human blood plasma. The deposition of calcium phosphate on the nanofiber surfaces was investigated through scanning electronic microscopy and X-ray diffraction. Energy dispersive spectroscopy results indicated that calcium-deficient hydroxyapatite had grown on the fibers. Fourier transform infrared spectroscopy analysis suggested the presence of hydroxyl-carbonate-apatite. The results of a viability assay (MTT) and alkaline phosphatase activity analysis suggested that these mineralized matrices promote osteogenic differentiation of human adipose-derived stem cells (hASCs) when cultured in an osteogenic medium and have the potential to be used as a scaffold in bone tissue engineering. hASCs cultured in the presence of nanofibers in endothelial differentiation medium showed lower rates of proliferation than cells cultured without the nanofibers. However, endothelial cell markers were detected in cells cultured in the presence of nanofibers in endothelial differentiation medium.

Increased Adipogenesis of Human Adipose-Derived Stem Cells on Polycaprolactone Fiber Matrices

“…A decreased proliferation rate was found over a range of seeding densities (S2A). This result corroborate previous studies reporting that use of PCL nanofiber matrices decreases proliferation of human adipose derived stem cells driven to osteogenesis [35]. Different initial seeding densities were tested in order to ensure that the larger surface area of the fiber-plates compared to conventional, 2D plates, did not influence the results.…”

“…Functionalized PCL fibers with molecular groups that stimulate bone formation, have been reported to improve osteogenesis starting only from mesenchymal stem cells [34], [35] and from adipose stem cells [36], [37].…”

Design, construction and mechanical testing of digital 3D anatomical data-based PCL–HA bone tissue engineering scaffold

“…The bone repairing would need to consider not only the choice of materials, but also the designability and manufacturability of the structure, so that the scaffold could not only exhibit the shape-similarity to the physical structure of bone, but also have the internal porous or multi-pipe connected structure that could promote the humoral mobility and cellular adherence [9,10]. Therefore, the current research hotspot is focused on the rapid prototyping (RP) technology, which is driven digitally to directly accumulate the materials to form the 3D solid model, and would not be limited by the shape complexity, and it could change and control the materials at any time during the manufacturing process, such as Woodfield et al [11] heated the PEGT/ PBT particles into a molten state, then pressure-extruded to form a complete knee stent, with the modulus as 0.05-2.5 MPa and the stiffness as 0.…”

Fabrication and Physical Evaluation of Gelatin-Coated Carbonate Apatite Foam

“…In fact, osteogenesis imperfecta, or Lobstein syndrome, is known to be caused by a deficiency in type I collagen [5,6]. Recently, a poly (L) lactic acid glycolic acid (PLGA) copolymer and ε-caprolactone were reported to be effective in CO 3 Ap foam reinforcement [7,8,9,10]. In both cases, the mechanical strength of the foam was improved significantly by coating it with these materials.…”