Differentiation of human dental pulp stem cells into functional motor neuron: In vitro and ex vivo study

Darvishi, Marzieh; Hamidabadi, Hatef Ghasemi; Bojnordi, Maryam Nazm; Saeednia, Sara; Zahiri, Maria; Niapour, Ali; Alizadeh, Rafieh

doi:10.1016/j.tice.2021.101542

Cited by 10 publications

(7 citation statements)

References 55 publications

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“…Therefore, there should be an optimal ECM protein that is suitable for DPSCs differentiation into neuronal cells. However, there is no research to investigate the effect of several ECM proteins as cell culture biomaterials on the differentiation of human DPSCs into neural cells from our database studies, although there are several studies ( Arthur et al, 2008 ; Ebrahimi et al, 2011 ; Zainal Ariffin et al, 2013 ; Chang et al, 2014 ; Feng et al, 2014 ; Kanafi et al, 2014 ; Al-Zer et al, 2015 ; Mattei et al, 2015 ; Cho et al, 2016 ; Young et al, 2016 ; Zhang et al, 2016 ; Geng et al, 2017 ; Sanen et al, 2017 ; Ullah et al, 2017 ; Zhang et al, 2017 ; Ganapathy et al, 2019 ; Hsiao et al, 2020 ; Rafiee et al, 2020 ; Solis-Castro et al, 2020 ; Arimura et al, 2021 ; Darvishi et al, 2021 ; Zheng et al, 2021 ) that address human DPSCs differentiation into neural cells.…”

Section: Introductionmentioning

confidence: 99%

Neuronal Cell Differentiation of Human Dental Pulp Stem Cells on Synthetic Polymeric Surfaces Coated With ECM Proteins

Gao

Tian

Liu

et al. 2022

Front. Cell Dev. Biol.

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Stem cells serve as an ideal source of tissue regeneration therapy because of their high stemness properties and regenerative activities. Mesenchymal stem cells (MSCs) are considered an excellent source of stem cell therapy because MSCs can be easily obtained without ethical concern and can differentiate into most types of cells in the human body. We prepared cell culture materials combined with synthetic polymeric materials of poly-N-isopropylacrylamide-co-butyl acrylate (PN) and extracellular matrix proteins to investigate the effect of cell culture biomaterials on the differentiation of dental pulp stem cells (DPSCs) into neuronal cells. The DPSCs cultured on poly-L-ornithine (PLO)-coated (TPS-PLO) plates and PLO and PN-coated (TPS-PLO-PN) plates showed excellent neuronal marker (βIII-tubulin and nestin) expression and the highest expansion rate among the culture plates investigated in this study. This result suggests that the TPS-PLO and TPS-PN-PLO plates maintained stable DPSCs proliferation and had good capabilities of differentiating into neuronal cells. TPS-PLO and TPS-PN-PLO plates may have high potentials as cell culture biomaterials for the differentiation of MSCs into several neural cells, such as cells in the central nervous system, retinal cells, retinal organoids and oligodendrocytes, which will expand the sources of cells for stem cell therapies in the future.

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Section: Introductionmentioning

confidence: 99%

Neuronal Cell Differentiation of Human Dental Pulp Stem Cells on Synthetic Polymeric Surfaces Coated With ECM Proteins

Gao

Tian

Liu

et al. 2022

Front. Cell Dev. Biol.

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“…Several studies indicate that intrinsic positional information can influence cell phenotype, and that neural crest populations can also produce mesenchymal derivatives confirming the role of the environment in orchestrating signals for neural crest development and differentiation [15,61]. Moreover, it is known that DPSCs express nestin, and low basal levels of markers associated with mature CNS cell types, such as the neuronal markers microtubule-associated protein 2 (MAP2), NF, β3-Tubulin, CNPase associated with oligodendrocytes, and astrocytic marker GFAP [35,36]. The data reported in the literature confirm that DPSC populations can be differentiated into neuron-like cells under appropriate conditions [9,14].…”

Section: Discussionmentioning

confidence: 85%

“…Moreover, recent studies have shown that DPSCs can also differentiate into neuron-like cells [3,34]. DPSCs are known to express nestin, a protein detectable in dividing cells during early development in the central nervous system (CNS), as well as peripheral (SNP), myogenic, and other tissues, and low basal levels of markers associated with mature CNS cell types, including the neuronal markers microtubule-associated protein 2 (MAP2), neurofilament (NF), β3-tubulin, CNPase associated with oligodendrocytes, and astrocytic marker glial fibrillary acid protein (GFAP) [35,36]. This pattern suggests that DPSC populations can indeed differentiate into neuron-like cells under appropriate conditions exhibiting typical electrophysiological properties [14,37,38].…”

Section: Introductionmentioning

confidence: 99%

Hypoxia Induces DPSC Differentiation versus a Neurogenic Phenotype by the Paracrine Mechanism

et al. 2022

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As previously described by several authors, dental pulp stem cells (DPSCs), when adequately stimulated, may acquire a neuronal-like phenotype acting as a favorable source of stem cells in the generation of nerves. Besides, it is known that hypoxia conditioning is capable of stimulating cell differentiation as well as survival and self-renewal, and that multiple growth factors, including Epidermal Growth factor (EGF) and basic fibroblast growth factor (bFGF), are often involved in the induction of the neuronal differentiation of progenitor cells. In this work, we investigated the role of hypoxia in the commitment of DPSCs into a neuronal phenotype. These cells were conditioned with hypoxia (O2 1%) for 5 and 16 days; subsequently, we analyzed the proliferation rate and morphology, and tested the cells for neural and stem markers. Moreover, we verified the possible autocrine/paracrine role of DPSCs in the induction of neural differentiation by comparing the secretome profile of the hypoxic and normoxic conditioned media (CM). Our results showed that the hypoxia-mediated DPSC differentiation was time dependent. Moreover, conditioned media (CM derived from DPSCs stimulated by hypoxia were able, in turn, to induce the neural differentiation of SH-SY5Y neuroblastoma cells and undifferentiated DPSCs. In conclusion, under the herein-mentioned conditions, hypoxia seems to favor the differentiation of DPSCs into neuron-like cells. In this way, we confirm the potential clinical utility of differentiated neuronal DPSCs, and we also suggest the even greater potential of CM-derived-hypoxic DPSCs that could more readily be used in regenerative therapies.

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“…Antibodies suitable for such proteins are usually used for fluorescence imaging [ 99 ]. Table 1 summarizes the studies that have monitored the differentiation process of stem cells over the past three years using immunocytochemistry [ 95 , 98 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 , 121 , 122 , 123 , 124 , 125 , 126 , 127 , 128 , 129 , 130 , 131 , 132 , 133 , 134 , 135 , 136 ]. ICC is a non-destructive method, different from Western blot.…”

Section: Latest Technological Advances In Monitoring Stem Cell Status...mentioning

confidence: 99%

Recent Advances in Monitoring Stem Cell Status and Differentiation Using Nano-Biosensing Technologies

et al. 2022

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In regenerative medicine, cell therapies using various stem cells have received attention as an alternative to overcome the limitations of existing therapeutic methods. Clinical applications of stem cells require the identification of characteristics at the single-cell level and continuous monitoring during expansion and differentiation. In this review, we recapitulate the application of various stem cells used in regenerative medicine and the latest technological advances in monitoring the differentiation process of stem cells. Single-cell RNA sequencing capable of profiling the expression of many genes at the single-cell level provides a new opportunity to analyze stem cell heterogeneity and to specify molecular markers related to the branching of differentiation lineages. However, this method is destructive and distorted. In addition, the differentiation process of a particular cell cannot be continuously tracked. Therefore, several spectroscopic methods have been developed to overcome these limitations. In particular, the application of Raman spectroscopy to measure the intrinsic vibration spectrum of molecules has been proposed as a powerful method that enables continuous monitoring of biochemical changes in the process of the differentiation of stem cells. This review provides a comprehensive overview of current analytical methods employed for stem cell engineering and future perspectives of nano-biosensing technologies as a platform for the in situ monitoring of stem cell status and differentiation.

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Differentiation of human dental pulp stem cells into functional motor neuron: In vitro and ex vivo study

Cited by 10 publications

References 55 publications

Neuronal Cell Differentiation of Human Dental Pulp Stem Cells on Synthetic Polymeric Surfaces Coated With ECM Proteins

Neuronal Cell Differentiation of Human Dental Pulp Stem Cells on Synthetic Polymeric Surfaces Coated With ECM Proteins

Hypoxia Induces DPSC Differentiation versus a Neurogenic Phenotype by the Paracrine Mechanism

Recent Advances in Monitoring Stem Cell Status and Differentiation Using Nano-Biosensing Technologies

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