Differentiation of human embryonic stem cells into smooth muscle cells in adherent monolayer culture

Huang, Huarong; Zhao, Xiaoli; Chen, Liangbiao; Xu, Chao; Yao, Xing; Lu, Yongliang; Dai, Licheng; Zhang, Ming

doi:10.1016/j.bbrc.2006.09.171

Cited by 83 publications

(70 citation statements)

References 27 publications

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“…4 Supported by a National Institutes of Health Research Supplement to Promote Diversity. 5 Supported by American Heart Association National Scientific Development Grant 0835237N. 6 An American Heart Association established investigator (Grant 0840025N).…”

Section: Methodsmentioning

confidence: 99%

“…The reason why ESCs are such a valuable tool is that each stage of early development is recapitulated in the process of ESC differentiation to a specific cell lineage (3,4). ESCs have been shown to be able to differentiate into smooth muscle cells (SMCs) through embryoid body formation under various differentiation conditions with combinations of molecular modifications (5)(6)(7)(8)(9)(10). We have recently developed a highly efficient and reproducible differentiation protocol, independent of embryoid body formation or additional molecular modifications, to generate relatively pure and functional SMC populations by treating ESCs with a high concentration of retinoid acid (RA) (5,7).…”

mentioning

confidence: 99%

“…ESCs have been shown to be able to differentiate into smooth muscle cells (SMCs) through embryoid body formation under various differentiation conditions with combinations of molecular modifications (5)(6)(7)(8)(9)(10). We have recently developed a highly efficient and reproducible differentiation protocol, independent of embryoid body formation or additional molecular modifications, to generate relatively pure and functional SMC populations by treating ESCs with a high concentration of retinoid acid (RA) (5,7). This ESC/SMC differentiation system has provided a valuable platform to explore molecular mechanisms of SMC differentiation in vitro, which can be instructive to in vivo disease models related to SMC differentiation.…”

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confidence: 99%

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miR-10a Contributes to Retinoid Acid-induced Smooth Muscle Cell Differentiation

Huang

Xie

Sun

et al. 2010

Journal of Biological Chemistry

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119

105

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MicroRNAs (miRs) have been reported to play a critical role in muscle differentiation and function. The purpose of this study is to determine the role of miRs during smooth muscle cell (SMC) differentiation from embryonic stem cells (ESCs). MicroRNA profiling showed that miR-10a expression is steadily increased during in vitro differentiation of mouse ESCs into SMCs. Lossof-function approaches using miR-10a inhibitors uncovered that miR-10a is a critical mediator for SMC lineage determination in our retinoic acid-induced ESC/SMC differentiation system. In addition, we have documented for the first time that histone deacetylase 4 is a novel target of miR-10a and mediates miR-10a function during ESC/SMC differentiation. To determine the molecular mechanism through which retinoic acid induced miR-10a expression, a consensus NF-B element was identified in the miR-10a gene promoter by bioinformatics analysis, and chromatin immunoprecipitation assay confirmed that NF-B could bind to this element. Finally, inhibition of NF-B nuclear translocation repressed miR-10a expression and decreased SMC differentiation from ESCs. Our data demonstrate for the first time that miR-10a is a novel regulator in SMC differentiation from ESCs. These studies suggest that miR-10a may play important roles in vascular biology and have implications for the diagnosis and treatment of vascular diseases.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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miR-10a Contributes to Retinoid Acid-induced Smooth Muscle Cell Differentiation

Huang

Xie

Sun

et al. 2010

Journal of Biological Chemistry

Self Cite

119

105

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“…Xie et al 77 treated iPSCs in vitro with retinoic acid (10 −5 M) forperiods and generate a lower SMC yield from both iPSCs and embryonic stem cells. 78,79 On successful differentiation, SMA-α-positive cells were seeded onto a 3-dimensional (3D) nanofibrous poly l-lactide scaffold for subcutaneous implantation into nude mice. Gene expression analysis revealed higher expression of some (myocardin and SM22α) but not all (smoothelin and smooth muscle myosin heavy chain) VSMC markers in the iPSC-VSMC-seeded scaffolds compared with controls after 2 weeks, showing incomplete iPSC differentiation.…”

Section: Tissue-engineered Blood Vesselsmentioning

confidence: 99%

Differentiation and Application of Induced Pluripotent Stem Cell–Derived Vascular Smooth Muscle Cells

Maguire

Xiao

2017

ATVB

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Abstract-Vascular smooth muscle cells (VSMCs) play a role in the development of vascular disease, for example, neointimal formation, arterial aneurysm, and Marfan syndrome caused by genetic mutations in VSMCs, but little is known about the mechanisms of the disease process. Advances in induced pluripotent stem cell technology have now made it possible to derive VSMCs from several different somatic cells using a selection of protocols. As such, researchers have set out to delineate key signaling processes involved in triggering VSMC gene expression to grasp the extent of gene regulatory networks involved in phenotype commitment. This technology has also paved the way for investigations into diseases affecting VSMC behavior and function, which may be treatable once an identifiable culprit molecule or gene has been repaired. Moreover, induced pluripotent stem cell-derived VSMCs are also being considered for their use in tissue-engineered blood vessels as they may prove more beneficial than using autologous vessels. Finally, while several issues remains to be clarified before induced pluripotent stem cell-derived VSMCs can become used in regenerative medicine, they do offer both clinicians and researchers hope for both treating and understanding vascular disease. In this review, we aim to update the recent progress on VSMC generation from stem cells and the underlying molecular mechanisms of VSMC differentiation. We will also explore how the use of induced pluripotent stem cell-derived VSMCs has changed the game for regenerative medicine by offering new therapeutic avenues to clinicians, as well as providing researchers with a new platform for modeling of vascular disease. Visual Overview-An online visual overview is available for this article.

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“…258,260,264 Using the EB method, both vascular cell types (ECs and SMCs) could be derived from hESCs and could generate vasculature on implantation into mice. 265 Using the same method, it was shown that the sequential treatment of EBs with BMP4, Activin A, FGF2, VEGFA, and SB431542 (a TGFbIIR inhibitor), resulted in a robust EC fate.…”

Section: Fig 2 Schematic Diagrammentioning

confidence: 99%