Differentiation of human influenza A viruses including the pandemic subtype H1N1/2009 by conventional multiplex PCR

Furuse, Yuki; Odagiri, Takashi; Okada, Takashi; Khandaker, Irona; Shimabukuro, Kozue; Sawayama, Rumi; Suzuki, Akira; Oshitani, Hitoshi

doi:10.1016/j.jviromet.2010.04.023

Cited by 9 publications

(9 citation statements)

References 19 publications

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“…This assay shows 97% accuracy and limit of detection (L.O.D) is 0.1-102 PFU/ml. This method is specific, accurate and highly sensitive for all strains of influenza A, but it also takes time [24][25][26][27]. Biosensors hold good capability to convert today's diagnostic methods into fast analytical powers by reconstituting their sensing behaviours for the detection of any nano-sized objects, like antibody, biomolecules and pathogens.…”

Section: Introductionmentioning

confidence: 99%

Detection methods for influenza A H1N1 virus with special reference to biosensors: a review

et al. 2020

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H1N1 (Swine flu) is caused by influenza A virus, which is a member of Orthomyxoviridae family. Transmission of H1N1 occurs from human to human through air or sometimes from pigs to humans. The influenza virus has different RNA segments, which can reassert to make new virus strain with the possibility to create an outbreak in unimmunized people. Gene reassortment is a process through which new strains are emerging in pigs, as it has specific receptors for both human influenza and avian influenza viruses. H1N1 binds specifically with an α-2,6 glycosidic bond, which is present in human respiratory tract cells as well as in pigs. Considering the fact of fast multiplication of viruses inside the living cells, rapid detection methods need an hour. Currently, WHO recommended methods for the detection of swine flu include real-time PCR in specific testing centres that take 3–4 h. More recently, a number of methods such as Antigen–Antibody or RT-LAMP and DNA biosensors have also been developed that are rapid and more sensitive. This review describes the various challenges in the diagnosis of H1N1, and merits and demerits of conventional vis-à-vis latest methods with special emphasis on biosensors.

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Section: Introductionmentioning

confidence: 99%

Detection methods for influenza A H1N1 virus with special reference to biosensors: a review

et al. 2020

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“…Thirteen respiratory viruses (influenza virus A and B, human respiratory syncytial virus, human metapneumovirus, parainfluenza virus types 1 to 4, human rhinoviruses, human coronaviruses (229E and OC43), adenovirus and human bocavirus) were screened on each specimen by multiplex hemi‐nested (RT)‐PCR as described previously [Tran et al, ]. Samples positive for influenza A were further subtyped as seasonal H1N1, seasonal H3N2, or H1N1pdm09 by multiplex nested PCR [Furuse et al, ].…”

Section: Methodsmentioning

confidence: 99%

Molecular epidemiology of influenza A virus infection among hospitalized children in vietnam during post‐pandemic period

Tran

Pham

et al. 2015

Journal of Medical Virology

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Genetic variability makes influenza virus to escape the immunity and causes yearly epidemics. Monitoring those changes is necessary for vaccine selection. In addition, H3N2 viruses were considered to be seeded from Southeast Asia before spreading globally. This study described the molecular epidemiology of influenza A during the post-pandemic season 2010-2011 in Vietnam. Nasopharyngeal samples were collected from children with respiratory infections at Children's Hospital 2, Ho Chi Minh City. The HA, NA, M genes were amplified, sequenced and analyzed. Thirty-five of 1,082 (3.2%) patients were positive for influenza A, including 14 pandemic H1N1 2009 (H1N1pdm09) and 21 H3N2 infections. H3N2 was dominant in the rainy season (May-October 2010) while H1N1pdm09 was dominant in the dry season (November 2010-April 2011). Phylogenetic analysis showed that Vietnamese H1N1pdm09 sequences in 2010-2011 formed the distinct cluster, with other contemporary Asian and 2012-American sequences, suggesting a possible common ancestor. All were oseltamivir-sensitive except two strains carrying S247N and D199N in NA which reduced the neuraminidase inhibitor susceptibility. The Vietnamese H3N2 viruses in mid-2010 belonged to the emerging subclade Perth10/2010, which then spread worldwide in 2011. The Vietnamese influenza viruses were well matched with the Southern Hemisphere vaccine formulation. Mutations at antigenic sites were also identified in these viruses. Surveillance of influenza viruses in tropical countries is important not only for development of their prevention and control strategies but also for earlier identification of the newly emerged strains that may be selected for future vaccine.

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“…Virus infection titres are expressed as log 10 plaque forming units/ml. Viral subtypes were determined by Multiplex PCR with four primers designed to differentiate between the pandemic H1N1 virus and seasonal H1N1 and H3N2 viruses [16].…”

Section: Virus Isolation and Titrationmentioning

confidence: 99%

Comparison between virus Shedding and Fever Duration after Treating Children with Pandemic a H1N1/09 and Children with a H3N2 with a Neuraminidase Inhibitor

Sugaya

Sakai‐Tagawa²,

Bamba³

et al. 2015

Antiviral Therapy

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Differentiation of human influenza A viruses including the pandemic subtype H1N1/2009 by conventional multiplex PCR

Cited by 9 publications

References 19 publications

Detection methods for influenza A H1N1 virus with special reference to biosensors: a review

Detection methods for influenza A H1N1 virus with special reference to biosensors: a review

Molecular epidemiology of influenza A virus infection among hospitalized children in vietnam during post‐pandemic period

Comparison between virus Shedding and Fever Duration after Treating Children with Pandemic a H1N1/09 and Children with a H3N2 with a Neuraminidase Inhibitor

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