Differentiation of <i>Anaplasmataceae</i> through Partial <i>groEL</i> Gene Analysis

Park, Hyo-Soon; Lee, Jung‐Hee; Jeong, Eunju; Park, Tae-Kyu; Kim, Tae-Yung; Chae, Joon‐Seok; Park, Jinho; Klein, Terry A.; Jang, Won‐Jong; Park, Kyung-Hee; Lee, Seung–Hyun

doi:10.1111/j.1348-0421.2005.tb03644.x

Cited by 16 publications

(14 citation statements)

References 18 publications

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“…The phylogeny of the groEL gene provides good evolutionary relationships among members of the eubacterial lineage [29], but some of the proposed tools require the supplementary step of a nested PCR to differentiate between species, yielding the possibility of contamination. The 16S rRNA gene sequence analysis is a good tool but requires approximately 1400 bp for a higher level of divergence [27]. In this study, using the higher level of divergence of the 23S rRNA gene sequence, we successfully developed rapid differentiation methods involving speciesspecific PCR based on the 23S rRNA gene sequences.…”

Section: Discussionmentioning

confidence: 99%

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Development of a new PCR-based assay to detect Anaplasmataceae and the first report of Anaplasma phagocytophilum and Anaplasma platys in cattle from Algeria

Dahmani

Davoust

Benterki

et al. 2015

Comparative Immunology, Microbiology and Infectious Diseases

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Section: Discussionmentioning

confidence: 99%

“…Most of them are based on the major surface proteins (MSPs) [26], the heat shock gene groEL [27] and the 16S rRNA gene [14]. The World Organisation for Animal Health (OIE) reports that laboratories running the MSPs PCR Assay should recognize problems associated with the inability to discriminate between some Anaplasma spp.…”

Section: Discussionmentioning

confidence: 99%

Development of a new PCR-based assay to detect Anaplasmataceae and the first report of Anaplasma phagocytophilum and Anaplasma platys in cattle from Algeria

Dahmani

Davoust

Benterki

et al. 2015

Comparative Immunology, Microbiology and Infectious Diseases

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“…The corresponding gene has been proposed as a target for molecular diagnostics for differentiation between members of the genus Rickettsia [13] and the family Anaplasmataceae [14]. Recently, Park et al.…”

Section: Introductionmentioning

confidence: 99%

A highly sensitive quantitative real-time PCR assay based on the groEL gene of contemporary Thai strains of Orientia tsutsugamushi

Paris

Aukkanit

Jenjaroen

et al. 2009

Clinical Microbiology and Infection

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Partial nucleotide sequences (459 bp) of the groEL gene (encoding the 60-kDa heat shock protein, HSP60) from 23 contemporary isolates of Orientia tsutsugamushi isolated from patients with acute scrub typhus in Thailand were compared with 16 reference strain sequences to evaluate the potential of groEL as a conserved and representative target for molecular diagnostics.. Overall nucleotide identity within all available O. tsutsugamushi isolates (n = 39) was 98.8% (range: 95.0–100), reflecting a high degree of conservation; nucleotide identities were 67.5% and 65.6%, respectively, when typhus and spotted fever group rickettsiae were included.. A highly sensitive and quantitative real-time PCR assay was designed and evaluated using 61 samples, including buffy coats from patients in Thailand and Laos. Reliable and accurate quantitation of bacterial loads allows further investigation of other diagnostic methods and may lead to an improved understanding of the pathophysiology of acute scrub typhus, an important but under-recognized disease.

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“…Le diagnostic des Anaplasmataceae est proposé en utilisant les gènes rrs, rrl, rpoB, gltA et groESl (Brouqui et al 2007). La plupart de ces systèmes ont besoin d'amplifier un (Park et al 2005 ;Viale et al 1994).…”

Section: Infections D'ovins Et De Caprins Sur La Côte Orientaleunclassified

Apport de la qPCR au dépistage des infections des ruminants par des Anaplasmataceae : étude de cas au Pays Basque et en Corse

Dahmani¹,

Mediannikov²,

Rousseau³

et al. 2016

bavf

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Les Anaplasmataceae sont des bactéries intracellulaires transmises aux animaux et à l’homme, principalement par les tiques. Les Anaplasma spp. (A. marginale, A. phagocytophilum…) et les Ehrlichia spp. (E. canis, E. ruminantium...) en sont les principaux représentants d’importance économique et sanitaire. Les outils de diagnostic disponibles jouent un rôle déterminant dans leur identification. Nous proposons une approche globale par un système permettant d’identifier la plupart des espèces d’Anaplasmataceae. Il est basé sur le gène codant l’ARNr 23S ; il couple une qPCR et une PCR standard avec deux jeux d’amorces suivie d’un séquençage. Il est testé pour en vérifier la spécificité, puis mis en oeuvre à partir de prélèvements de sang de ruminants et de tiques : dans les Pyrénées-Atlantiques, des brebis suspectes d’infection sont porteuses d’A. ovis et leurs tiques Rhipicephalus bursa, d’A. ovis, d’A. phagocytophilum et d’une nouvelle ehrlichia. En Haute-Corse, une forte prévalence d’infections par les Anaplasmataceae est détectée dans des élevages (bovins, ovins, caprins) où la symptomatologie rétrocède lors de traitements à l’oxytétracycline. La spécificité et la sensibilité du système de diagnostic utilisé et sa capacité à identifier de nouvelles espèces offrent des perspectives pour l’étude de l’épidémiologie des Anaplasmataceae et permettront de mettre en évidence de nouveaux réservoirs.

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Differentiation of Anaplasmataceae through Partial groEL Gene Analysis

Abstract: The nucleotide sequences (287 bp) of the partial groEL gene from 14 reference strains of Anaplasmataceae were determined and compared. A partial groEL gene is useful for the identification and characterization of Anaplasmataceae, in spite of its short nucleotide sequences.

Cited by 16 publications

References 18 publications

Development of a new PCR-based assay to detect Anaplasmataceae and the first report of Anaplasma phagocytophilum and Anaplasma platys in cattle from Algeria

Development of a new PCR-based assay to detect Anaplasmataceae and the first report of Anaplasma phagocytophilum and Anaplasma platys in cattle from Algeria

A highly sensitive quantitative real-time PCR assay based on the groEL gene of contemporary Thai strains of Orientia tsutsugamushi

Apport de la qPCR au dépistage des infections des ruminants par des Anaplasmataceae : étude de cas au Pays Basque et en Corse

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