Differentiation of
            <i>Aspergillus ustus</i>
            Strains by Random Amplification of Polymorphic DNA

Rath, Peter‐Michael; Petermeier, Katrin; Verweij, Paul E.; Ansorg, R.

doi:10.1128/jcm.40.6.2231-2233.2002

Cited by 17 publications

(12 citation statements)

References 13 publications

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“…This is, further, a demonstration of the high intraspecific genetic diversity of A. flavus. The results shown in the present study support the use of RAPD fingerprinting for analyzing A. flavus strains using different primers, as demonstrated before for A. niger (Megnegneau et al, 1993), A. fumigatus (Verweij et al, 1996;Bertout et al, 2001;Bart-Delabesse et al, 2001;Lasker, 2002), A. flavus (Geiser et al, 1998;Diaz-Guerra et al, 2000), A. terreus (Rath et al, 1999), A. nidulans (Rath, 2001), and A. ustus (Rath et al, 2002). It is also true for other species, such as the fumonisin producer Fusarium moniliforme (Jimenez et al, 2000).…”

Section: Rapd Analysissupporting

confidence: 88%

Genetic characterization of Brazilian strains of Aspergillus flavus using DNA markers

Batista

Santos²,

Oliveira³

et al. 2008

Genet. Mol. Res.

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ABSTRACT. The Aspergillus genus belongs to a filamentous fungal group characterized by wide dispersion in the environment. Some species are associated with diseases, especially in immunocompromised patients, while others are of economical importance due to aflatoxin production or biotechnological applications. Its species identification is nowadays performed by traditional techniques combined with molecular markers, resulting in a higher efficiency of isolate characterization. In the present study, internal transcribed spacer, inter-simple sequence repeats (ISSR), and random amplified polymorphic DNA (RAPD) molecular markers were used, with the aim of genetically characterizing strains of Aspergillus flavus and strains of other species of the A. flavus group. High genetic diversity was revealed by RAPD and by ISSR, in which the use of the (GACA) 4 primer yielded a higher diversity than with the (GTG) 5 primer, although the latter showed a characteristic banding profile for each species. These data were used to create a similarity matrix for the construction of dendrograms by means of the UPGMA method. The ISSR and RAPD profiles showed that among the strains previously identificated as A. flavus, one should be A. oryzae, one A. parasiticus and two A. tamarii. On the other hand, a strain previously identified as A. parasiticus should be A. flavus. All these strains 707 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 7 (3): 706-717 (2008) Genetic characterization of Aspergillus flavus strains were retested by traditional methods and their new species identification was confirmed. These results strongly support the need for using molecular markers as an auxiliary tool in differentiating fungal species and strains.

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Section: Rapd Analysissupporting

confidence: 88%

Genetic characterization of Brazilian strains of Aspergillus flavus using DNA markers

Batista

Santos²,

Oliveira³

et al. 2008

Genet. Mol. Res.

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“…These results accord with the outcome of studies performed by other workers in the molecular typing of various species of Aspergillus [3,7,10,13,17,22,24]. The RAPD method is doubtless emerging as a high-utility tool in Wngerprinting the genome of pathogenic fungi species.…”

Section: Resultssupporting

confidence: 93%

“…Phenotypic techniques are based on identiWcation of phenotypic characters, and encompass antigenic proWling, immunoelectrophoresis, radioimmunoelectrophoresis, immunoblotting, enzyme electrophoresis, and yeast killer toxin typing systems [4]. Genotypic techniques involve genomic DNA characterization, and encompass a range of techniques like restriction length fragment polymorphism (RFLP), electrophoretic karyotyping, multilocus enzyme electrophoresis, and PCRbased Wngerprinting methods including microsatellite PCR, Southern hybridization probe, as well as the rapid ampliWcation of polymorphic DNA (RAPD) [4,17,20].…”

Section: Introductionmentioning

confidence: 99%

Differentiation of Aspergillus niger by random amplification of polymorphic DNA

Abed

2008

J Ind Microbiol Biotechnol

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The randomly amplified polymorphic DNA (RAPD) patterns of whole-cell lysates from five Aspergillus niger isolates, including one reference strain, two isolated from deep freeze, and two environmental strains from soil and plant infections, were investigated. PCR-RAPD analysis of genomic DNA was performed using eight primers (Tube-A1, Tube-A6, Tube-A17, Tube-B8, Tube-B11, Tube-B15, Tube-C5, Tube-C6). The RAPD assay discriminated between all strains. Comparison of deep freeze isolates showed identical RAPD patterns in some of the reference and environmental isolates. The data indicates that the RAPD technique is useful for fingerprinting A. niger.

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“…A. ustus isolates may vary in their colony color from mud brown to slate gray, with colony reverse colors from uncolored through yellow to dark brown (14,23). Molecular data, including randomly amplified polymorphic DNA analysis (20,24) and sequence analysis of parts of the rRNA gene cluster (8,10,22), also indicate that this species is highly variable. These molecular and chemical data indicate that more species may be present in the broad description of A. ustus provided by Raper and Fennell (23).…”

mentioning

confidence: 99%

Aspergillus calidoustus sp. nov., Causative Agent of Human Infections Previously Assigned to Aspergillus ustus

et al. 2008

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Aspergillus ustus is a relatively rare human pathogen causing invasive infections in immunocompromised hosts. In this study isolates originating from clinical and other sources have been examined using molecular, morphological, and physiological approaches to clarify their species assignment. Phylogenetic analysis of partial ␤-tubulin, calmodulin, actin, and intergenic transcribed spacer sequences indicated that none of the clinical isolates recognized previously as A. ustus belongs to this species. All but two of these isolates formed a well-defined clade related to A. pseudodeflectus based on sequence analysis of protein-coding regions. Morphological and physiological examination of these isolates indicated that they are able to grow above 37°C, in contrast with A. ustus isolates, and give a positive Ehrlich reaction, in contrast with related species including A. granulosus, A. ustus, and A. pseudodeflectus. These isolates are proposed as a new species, A. calidoustus. Antifungal susceptibility testing showed that this species has decreased susceptibilities to several antifungal drugs. The triazoles are inactive in vitro, including the new azole posaconazole.

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Differentiation of Aspergillus ustus Strains by Random Amplification of Polymorphic DNA

Cited by 17 publications

References 13 publications

Genetic characterization of Brazilian strains of Aspergillus flavus using DNA markers

Genetic characterization of Brazilian strains of Aspergillus flavus using DNA markers

Differentiation of Aspergillus niger by random amplification of polymorphic DNA

Aspergillus calidoustus sp. nov., Causative Agent of Human Infections Previously Assigned to Aspergillus ustus

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