Differentiation of Leydig cells in the Mongolian gerbil

Abstract

Information on postnatal Leydig cell (LC) differentiation in the Mongolian gerbil has been unavailable. Therefore, current investigation was designed to examine LC lineage differentiationin this rodent, from birth to adulthood. Gerbil testes at 1 day, 1-7 weeks (w), 2 and 3 months of age were conventionally processed by light and transmission electron microscopy. Immunocytochemistry for specific markers of steroidogenic enzymes, namely 3beta-hydroxysteroid dehydrogenase (3beta-HSD) and 11beta-hydroxysteroid steroid dehydrogenase 1 (11beta-HSD1) and also for androgen receptor (AR) was performed. The establishment of adult Leydig cell populations (ALC) during testis maturation in the gerbil follows the pattern previously described in other mammalian species, with the four progressive stages of differentiation. The LC progenitors were identified at second w by 3beta-HSD expression; the first newly formed ALC were recognized at fourth w whereas immature ALC appeared at fifth w. The latter were recognized by abundance of cytoplasmic lipid, in addition to expression of 11beta-HSD1 and intense nuclear AR immunoreaction. Mature ALC in gerbil exhibited irregular eccentric nuclei and a cytoplasmic canaliculus in the perinuclear area. Only one third of mature ALC in adult gerbils showed a high expression of 11beta-HSD1 and AR expression was highly variable among them. In conclusion, the process of differentiation of ALC population in gerbil follows the pattern previously established for other rodents. However, the resulting mature ALC are strikingly different due their singular asymmetric morphology and presence of a cytoplasmic canaliculus and as well as their functional heterogeneity.

Hydroxysteroid Dehydrogenases – Localization, Function and Regulation in the Testis

“…In previous studies, activity of HSDs in testis of various mammals was mostly detected using histochemical techniques (Badrinarayanan et al, 2006;Bilinska 1979Bilinska , 1983Bilinska , 1994Hutson, 1989), (Figure 2). Nowadays the resolution of their localization increased with applying specific antibodies Pelletier et al, 1999;Pinto et al, 2010).…”

“…One develops prenatally (fetal Leydig cells, FLCs) and the second arises postnatally (adult Leydig cells, ALCs), (Mendis-Handagama & Aryiaratne, 2001;Pinto et al, 2010). These two generations have different gene expression profiles, which indicate that they originate from separate types of stem cell (Dong et al, 2007;O'Shaugnessy et al, 2002b).…”