Differentiation of Live from Dead Spermatozoa in Cock Semen

Wilson, H. R.; Warnick, A. C.; Gutiérrez, Jara

doi:10.3382/ps.0480714

Cited by 15 publications

(11 citation statements)

References 7 publications

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“…The percent dead sperm (Table 3) did not have the detrimental effect on fertility which had been expected based on earlier artificial insemination studies using Leghorns (Wilson et al, 1969b;Wilson and Rowland, 1370;. Some effect appeared to be present in Experiment 3 but little apparent relationship in the other experiments.…”

Section: Current Experimentsmentioning

confidence: 50%

“…Concentration was determined using a Coulter Counter procedure similar to that described by . Percent dead sperm was determined by the trypan blue wet mount method (Wilson et al, 1969b). Motility was scored from 1 to 6, with 1 having no motility and 6 being all cells motile and swirling.…”

Section: Florida Studies Background and General Proceduresmentioning

confidence: 99%

“…In a study of live-dead staining techniques, Wilson et al, (1969b) observed a significant decrease in fertility from articfiial insemination when the number of dead sperm exceeded 1 %. However, a wide range in percent dead sperm existed without a significant effect on fertility.…”

Section: Florida Studies Background and General Proceduresmentioning

confidence: 99%

“…For a classification of the various types of abnormalities occurring in fowl sperm one should refer to articles by Parker et al (1942) and Lake (1954). Percent dead has also been shown to be significantly related to reduction time (Marini and Goodman, 1969;McDaniel and Craig, 1962) and oxygen uptake in a Warburg apparatus (Wilson et al, 1969b). Ogasawara et al (1966) reported that in some instances cocks with large numbers of abnormal sperm (morphology, motility and metabolism) have low fertility due to the failure of such sperm to reach the utero-vaginal sperm nests.…”

Section: Introduction and Literature Reviewmentioning

confidence: 99%

See 3 more Smart Citations

Prediction of the Fertility Potential of Broiler Breeder Males

Wilson¹,

Piesco²,

Miller³

et al. 1979

World's Poultry Science Journal

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Semen Quality Characteristics and FertilityThe selection of males for breeder flocks is usually based on such characteristics as posture, body conformation, maturity, body size, ~~~~ ~ ~ ~ *Ha. Agr. Exp. Sta. Journal Series No. 1062. 95 96condition of the legs and feet, appearance of the head, general health and vigour, and appearance of eyes. Although these characteristics are useful in selecting males they do not assure that high fertility will be obtained. Several attempts have been made to relate various semen quality characteristics to fertility. There is much disagreement in the literature, however, regarding the importance and reliability of semen traits in predicting fertility. Differences can, of course, be expected between the results obtained from natural mating and artificial insemination. Although measurements of semen traits have been succcssful in detecting sterile birds, the prediction of fertilizing capacity appears to be complex and influenced by numerous factors other semen quality.In order to make fertility predictions or male breeding worth predictions based on semen evaluation, high correlations between semen characteristics and fertility must be found. Then a statistical model may be fitted using the semen quality values as independent variables to define the dependent variable, fertility. In addition, when attempting to obtain an estimate of fertility or breeding potential, one must keep in mind that it is influenced by genetical, physiological and social factors.Semen quality characteristics are generally believed to be inherited (Lake, 1971; Marini and Goodman, 1969) and differences in semen characteristics and fertility have been reported between breeds (Parker et al., 1942;Allen and Champion, 1955; Taneja and Gowe, 1961 a and b;McDaniel and Craig, 1962), between inbred lines (Williams and McGibbon, 1956; De-Silva, 1963) and between families (Boone, 1968).Heritability values for such traits as semen quantity, concentration and motility were found to be high (Soller et al. 1965b) as were the repeatabilities of such measurements. Nestor and Brown (1976a) reported that measurements of semen quality made early in the reproductive life of the male were not closely correlated with subsequent evaluations and did not permit accurate prediction of future semen production. Nestor (1976) successfully selected turkeys for increased semen yield, however, there were no consistent changes in percent fertility, percent hatchability of fertile eggs or number of poults per hen associated with the increases in semen yield. Some success also has been reported for the selection for fertility when using frozen chicken semen (Scott et al., 1977).Concentration of spermatozoa in semen has been claimed to be significantly related to fertility (following AI), having a correlation coefficient of 321 (Kamar, 1960) and .481 (Boone, 1968). Others have presented data indicating that it is not significantly related to fertility (

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Section: Current Experimentsmentioning

confidence: 50%

Section: Florida Studies Background and General Proceduresmentioning

confidence: 99%

Section: Florida Studies Background and General Proceduresmentioning

confidence: 99%

Section: Introduction and Literature Reviewmentioning

confidence: 99%

See 2 more Smart Citations

Prediction of the Fertility Potential of Broiler Breeder Males

Wilson¹,

Piesco²,

Miller³

et al. 1979

World's Poultry Science Journal

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show abstract

“…3. Percentage of dead sperm was deter-mined with the trypan blue technique described by Wilson et al (1969). 4.…”

Section: Methodsmentioning

confidence: 99%

Effect of Restricted Daylength During the Growing Period on Semen Quality and Fertility of Turkey Toms

Wall

Jones

1976

Poultry Science

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Two trials were conducted in which three daylength and light intensity treatments were applied to 144 growing turkey toms from 8 to 28 weeks of age. Treatments were (1) six hours of artificial light, (2) natural light and daylength, and (3) simulated natural daylength. At 28 weeks of age all toms were placed on 14 hours of daily light. Individual tom semen quality was evaluated every two weeks for six months, and on alternate weeks, eight hens were inseminated with semen pooled by treatment replicate to obtain fertility (May to December). Seasonal decline in semen production, sperm concentration, semen volume, and fertility was observed for toms on natural daylength after three months of production. Toms on six hours of light and those on simulated natural light maintained high levels of production and quality for the entire six month period. Semen production expressed as the average number of toms producing semen, sperm concentration, and fertily were significantly lower (P less than .01), and semen volume was significantly lower (P less than .05) for toms on natural light after six months. It was concluded that natural light was less desirable than controlled light for "off season" growing of toms.

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Lead toxicity of Bobwhite quail

Damron¹,

Wilson²

1975

Bull. Environ. Contam. Toxicol.

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Five experiments were conducted involving both juvenile and adult Bobwhite quail (Colinus virginianus) to study the toxicity of lead acetate, lead shot, powdered lead and antimony. Performance of juvenile birds receiving up to 1,500 p.p.m. of lead as lead acetate was not significantly influenced during the six-week experimental period. Feeding 3,000 p.p.m. lead as lead acetate was not significantly influenced during the six-week experimental period. Feeding 3,000 p.p.m. lead as lead acetate was associated with a significant depression of body weight and an increase in mortality. Feeding growing quail up to 2,000 p.p.m. lead from either lead acetate or powdered lead, 3 or 6% antimony (as % of shot dosage or 5 lead shot per week caused no significant effects upon either body weight or mortality. No significant trends were noted in body weight, feed intake, semen quality or organ weight of adult quail that received up to 1,500 p.p.m. lead from lead acetate. Dosing adult Bobwhite quail cocks with 10 or more lead shot per week caused a significant increase in nortality, and more than 90% of those dosed with 30 shot per week died by the end of four weeks.

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Differentiation of Live from Dead Spermatozoa in Cock Semen

Cited by 15 publications

References 7 publications

Prediction of the Fertility Potential of Broiler Breeder Males

Prediction of the Fertility Potential of Broiler Breeder Males

Effect of Restricted Daylength During the Growing Period on Semen Quality and Fertility of Turkey Toms

Lead toxicity of Bobwhite quail

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