Differentiation of liver epithelial (stem-like) cells into hepatocytes induced by coculture with hepatic stellate cells

Nagai, Hirokazu; Terada, Kunihiko; Watanabe, Go; Ueno, Yasuharu; Aiba, Namiko; Shibuya, Tomomi; Kawagoe, Makoto; Kameda, Takashi; Sato, Mitsuru; Senoo, Haruki; Sugiyama, Toshihiro

doi:10.1016/s0006-291x(02)00406-0

Cited by 63 publications

(48 citation statements)

References 17 publications

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“…Additionally, significant engraftment of bone marrowderived cells during liver injury is thought to require in vivo selection. However, Yamamoto et al [24,25] support the notion that bone marrow ablation is not required for stem cell engraftment in the liver. Mononuclear bone marrow cells include mesenchymal stem cells (MSC), hematopoietic stem cells (HSC), and many kinds of cell factors.…”

Section: Discussionmentioning

confidence: 99%

Contribution of mononuclear bone marrow cells to carbon tetrachloride-induced liver fibrosis in rats

Cao

Lin²,

Zhong³

et al. 2007

WJG

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AIM:To study the inhibitory effect of mononuclear bone marrow cell (BMC) transplantation on carbon tetrachloride (CCl4) -induced liver fibrosis in rats. METHODS:Rat liver fibrosis models were induced by CCl4 and alcohol administration. After 8 wk, twenty rats were randomly allocated into treatment group (n = 10) and control group (n = 10). BMC were infused into the rats in treatment group via the portal vein, while heparinized saline was infused in control group. CCl4 was hypodermically injected into the rats twice a week for 4 wk. At the end of wk 12, all rats were humanely sacrificed. Liver samples were taken and stained with HE or Masson trichrome. The general conditions, liver fibrosis (hydroxyproline and collagen fibre) and liver pathological grades in rats were evaluated. RESULTS:The general conditions of the rats in treatment group improved markedly, but not in control group. Hydroxyproline was 504.6 ± 128.8 mg/g in treatment group, and 596.0 ± 341.8 mg/g in control group. The percentage of collagen fibre was 3.75% ± 0.98% in treatment group and 5.02% ± 0.44% in control group. There was a significant difference between the two groups (P < 0.05). Liver pathological grade decreased from grade Ⅳ to grade Ⅲ partially i n t r e a t m e n t g r o u p (P < 0 . 0 5 ) w i t h n o o b v i o u s improvement in control group (P > 0.05). There was a significant difference between treatment group and control group (P < 0.05). World

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Section: Discussionmentioning

confidence: 99%

Contribution of mononuclear bone marrow cells to carbon tetrachloride-induced liver fibrosis in rats

Cao

Lin²,

Zhong³

et al. 2007

WJG

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“…The role of hepatic stellate cells in hepatoblast differentiation is less clear. Nagai et al reported that cell-cell contacts between hepatic stellate cells and hepatic epithelial cells induce the differentiation of the hepatocyte fate (61). On the other hand, the emergence and distribution of hepatic stellate cells also seem to correlate with the development of intrahepatic biliary cells (62).…”

Section: Hepatic Stellate Cells In Liver Developmentmentioning

confidence: 99%

Hepatic stellate cells in liver development, regeneration, and cancer

Yin¹,

Evason²,

Asahina³

et al. 2013

J. Clin. Invest.

604

567

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Hepatic stellate cells are liver-specific mesenchymal cells that play vital roles in liver physiology and fibrogenesis. They are located in the space of Disse and maintain close interactions with sinusoidal endothelial cells and hepatic epithelial cells. It is becoming increasingly clear that hepatic stellate cells have a profound impact on the differentiation, proliferation, and morphogenesis of other hepatic cell types during liver development and regeneration. In this Review, we summarize and evaluate the recent advances in our understanding of the formation and characteristics of hepatic stellate cells, as well as their function in liver development, regeneration, and cancer. We also discuss how improved knowledge of these processes offers new perspectives for the treatment of patients with liver diseases.

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“…In particular, there is evidence for secretion of hepatocyte growth factor by hepatic stellate cells [24], periportal connective tissue cells, and endothelial cells of the fetal liver [78] and for stimulation of the survival and proliferation of hepatic stem cells by stellate cells [79] or endotheliocytes [80] in cocultures. Paracrine signals from stellate cells stimulate hepatocytic differentiation in vitro [81], and co-culturing of hepatocytes with a certain fibroblast population induces them to form hepatic cordlike structures [82]. There is also evidence that the interaction of portal myofibroblasts and epithelium is important for the development of intrahepatic bile ducts [83].…”

Section: Stromal Regulation Of Hepatogenesismentioning

confidence: 99%

Hematopoietic Microenvironment in the Fetal Liver: Roles of Different Cell Populations

Payushina

2012

ISRN Cell Biology

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Hematopoiesis is the main function of the liver during a considerable period of mammalian prenatal development. Hematopoietic cells of the fetal liver exist in a specific microenvironment that controls their proliferation and differentiation. This microenvironment is created by different cell populations, including epitheliocytes, macrophages, various stromal elements (hepatic stellate cells, fibroblasts, myofibroblasts, vascular smooth muscle and endothelial cells, mesenchymal stromal cells), and also cells undergoing epithelial-to-mesenchymal transition. This paper considers the involvement of these cell types in the regulation of fetal liver hematopoiesis.

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Differentiation of liver epithelial (stem-like) cells into hepatocytes induced by coculture with hepatic stellate cells

Cited by 63 publications

References 17 publications

Contribution of mononuclear bone marrow cells to carbon tetrachloride-induced liver fibrosis in rats

Contribution of mononuclear bone marrow cells to carbon tetrachloride-induced liver fibrosis in rats

Hepatic stellate cells in liver development, regeneration, and cancer

Hematopoietic Microenvironment in the Fetal Liver: Roles of Different Cell Populations

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