Differentiation of marine luminous bacteria using commercial identification plates

Abstract

Differentiation of marine luminous bacteria using Biology GN plate combined with API 20e or the BBL Crystal ID plate inoculated with cell suspensions in artificial seawater was accomplished by comparison to type species using cluster analysis. Inoculum density affected the results from Biolog GN plates, but had less of an effect on the reactions obtained from API 20e strip or BBL Crystal ID plate. In a few cases, combination of the Biolog GN traits along with either the API 20e or Crystal ID traits was necessary to differentiate some marine luminous bacteria.

Roseimaritima ulvae gen. nov., sp. nov. and Rubripirellula obstinata gen. nov., sp. nov. two novel planctomycetes isolated from the epiphytic community of macroalgae

“…Growth was evaluated by measuring the turbidity of the cultures at 600 nm. To inoculate GN2 MicroPlate test strips, liquid cultures were grown for 48h, centrifuged and resuspended in sterile 0.75% ASW without CaCl 2 [17] and results were examined after 10 days. API 50 CH system was inoculated Polar lipids were extracted from freeze-dried cells as described previously [6].…”

A comparison of the bacterial microflora between coastal sites in Qingdao, P. R. China and Loch Fyne, Scotland

“…More recently, Montes et al (1999) studied 473 isolates from the skin of turbot (Scophthalmus rnaa:imus ) for 90 phenotypic traits, and distinguished many groups of vibrios, including V. albensis, V. anguillarum, V. fischeri, V. ordalii, V. scophthalmi and V. splendidus. It is apparent that there has been an increasing use of commercial identification systems in modern large scale numerical taxonomic studies, including API Rapid NFT (Breschel and Singleton, 1992), API 20E and BIOLOG-GN (Austin et al, 1995;Austin et aL, 1997;Makemson et aL, 1998;Ivanova et aL, 1998) and locally made variants (Hansen and Sorheim, 1991). These rapid methods have enabled standardisation of testing regimes, are labour saving, and so have allowed large numbers of isolates to be examined expeditiously.…”

The Function of Cyclo(Phe-Pro) in Gene Expression of Vibrio Harveyi

“…These plates are used to distinguish between strains of marine luminous bacteria (Makemson et al 1998). The Wild-Type strains (MAV and BB120) were streaked overnight onto agar plates of Glycerol Marine Agar (GMA: 0.5% peptone, 0.1% yeast extract, 0.02% glycerol phosphate, 0.3% glycerol, and 75% ASW) for the control suspensions.…”