2009
DOI: 10.1002/jcb.22442
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Differentiation of mouse embryonic stem cells into hepatocytes induced by a combination of cytokines and sodium butyrate

Abstract: There is increasing evidence to suggest that embryonic stem cells (ESCs) are capable of differentiating into hepatocytes in vitro. In this study, we used a combination of cytokines and sodium butyrate in a novel three-step procedure to efficiently direct the differentiation of mouse ESCs into hepatocytes. Mouse ESCs were first differentiated into definitive endoderm cells by 3 days of treatment with Activin A. The definitive endoderm cells were then differentiated into hepatocytes by the addition of acidic fib… Show more

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Cited by 26 publications
(20 citation statements)
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“…Gene silencing associated with the aberrant methylation of promoter region of CpG islands is an acquired epigenetic alteration that serves as an alternative to genetic events in the inactivation of tumor suppressor and other genes in human cancers. NaBu has been applied in cellular and molecular research (23,24), particularly in antitumor research (25)(26)(27)(28). It plays a vital role in vitamin D-induced apoptosis through PTEN upregulation, which has potential benefit in gastric cancer therapy (29).…”
Section: Discussionmentioning
confidence: 99%
“…Gene silencing associated with the aberrant methylation of promoter region of CpG islands is an acquired epigenetic alteration that serves as an alternative to genetic events in the inactivation of tumor suppressor and other genes in human cancers. NaBu has been applied in cellular and molecular research (23,24), particularly in antitumor research (25)(26)(27)(28). It plays a vital role in vitamin D-induced apoptosis through PTEN upregulation, which has potential benefit in gastric cancer therapy (29).…”
Section: Discussionmentioning
confidence: 99%
“…Among these methods, the stepwise addition of two or more factors is most common. [19][20][21][22][23] However, if these complicated operations are not performed adequately, the death of ESCs can easily occur. In previous culture systems, remarkable reduction of the number of live cells was a technical obstacle to the induction of differentiation.…”
Section: Discussionmentioning
confidence: 99%
“…Cui combined epigenetic modification with cytokines gradually added to the cells in order to induce embryonic stem cells into hepatocytes using sodium butyrate and activin A treatment, achieving a production rate of functional liver cells of 10%-70% (Hay et al, 2008). The induction rate was improved through a novel three-step process with activin A, aFGF and sodium butyrate in recently reported work (Zhou et al, 2010). Sodium butyrate is a histone deacetylase inhibitor which promotes histone acetylation and opening of the chromatin structure; therefore a certain number of vital genes regulating cellular growth and terminal differentiation are activated for transcription (Zhou et al, 2010).…”
Section: Induction By Chromatin Modificationmentioning
confidence: 99%
“…The induction rate was improved through a novel three-step process with activin A, aFGF and sodium butyrate in recently reported work (Zhou et al, 2010). Sodium butyrate is a histone deacetylase inhibitor which promotes histone acetylation and opening of the chromatin structure; therefore a certain number of vital genes regulating cellular growth and terminal differentiation are activated for transcription (Zhou et al, 2010). Thus chromatin modulation is an important aspect of initiating gene transcription that impacts hepatocyte differentiation.…”
Section: Induction By Chromatin Modificationmentioning
confidence: 99%