Differentiation of murine B cells induced by chondroitin sulfate B

Yoshihara, Ritsuko; Aoyama, Eriko; Kadota, Yusuke; Kawai, Saeko; Goto, Tomomi; Ming, Zhong; Gohda, Eiichi

doi:10.1016/j.cellimm.2007.12.002

Cited by 13 publications

(13 citation statements)

References 40 publications

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“…These findings are in agreement with published results. 15,16 On further examination, we found that DS specifically promotes the expansion of and antibody production by B-1a cells.…”

Section: Discussionmentioning

confidence: 82%

“…This observation was in agreement with our earlier assessment 14 and later reports. 15,16 The proliferative effect of DS was dose dependent, and concentrations of 20 g/mL or higher significantly stimulated cell proliferation. Moreover, the effect of DS was robustly observed with spleen cells from young or old mice, naive mice, or mice that had been previously immunized with a variety of antigens.…”

Section: Ds Stimulates Cd5 ϩ B-cell Proliferationmentioning

confidence: 99%

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Dermatan Sulfate Interacts with Dead Cells and Regulates CD5+ B-Cell Fate

Wang

Lee

Yan

et al. 2011

The American Journal of Pathology

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CD5 ؉ (B-1a) B cells play pivotal roles in autoimmunity through expression of autoreactive B-cell receptors and production of autoantibodies. The mechanism underlying their positive selection and expansion is currently unknown. This study demonstrates that dermatan sulfate (DS) expands the B-1a cell population and augments the specific antibody response to an antigen when it is in complex with DS. DS displays preferential affinity for apoptotic and dead cells, and DS-stimulated cell cultures produce antibodies to various known autoantigens. The companion article further illustrates that autoantigens can be identified by affinity to DS, suggesting that molecules with affinity to DS have a high propensity to become autoantigens. We thus propose that the association of antigens from dead cells with DS is a possible origin of autoantigens and that autoreactive B-1a cells are positively selected and expanded by DS•autoantigen complexes. This mechanism may also explain the clonal expansion of B-1a cells in certain B-cell malignancies.

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“…These findings are in agreement with published results. 15,16 On further examination, we found that DS specifically promotes the expansion of and antibody production by B-1a cells.…”

Section: Discussionmentioning

confidence: 82%

Section: Ds Stimulates Cd5 ϩ B-cell Proliferationmentioning

confidence: 99%

Dermatan Sulfate Interacts with Dead Cells and Regulates CD5+ B-Cell Fate

Wang

Lee

Yan

et al. 2011

The American Journal of Pathology

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“…9) Enzyme-linked Immunospot (ELISPOT) Assay --The ELISPOT assay was performed as described previously. 10) Briefly, to the 96-well microtiter plate coated with goat anti-mouse IgM Ab, enriched B cells, which had been incubated with or without the extract of red bell pepper for 5 days and washed with and suspended in the basal culture medium, were added (2 × 10 3 viable cell/200 µl per well) in quintuplicate and incubated for 2 hr at 37 • C. After being washed three times, the plate was incubated with 50 µl of HRP-conjugated goat anti-mouse IgM Ab (0.2 µg/ml) for 1 hr at room temperature. After washing, 100 µl of substrate solution consisting of 50 mM sodium phosphate buffer (pH 7.0), 1% agarose, 2 mg/ml of diaminobenzidine, 0.0018% H 2 O 2 , 0.0018% NiCl 2 , and 0.0018% CoCl 2 was added.…”

Section: Antibody Response Of Enriched B Cells Ormentioning

confidence: 99%

Enhancement of Immunoglobulin M Production in B Cells by the Extract of Red Bell Pepper

Goto

Sarker

Ming

et al. 2010

JOURNAL OF HEALTH SCIENCE

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Under immunocompromised conditions such as aging, cancer, diabetes, burns, and sepsis, external agents may be useful for upregulation of the immune system to prevent infection. Dietary products have the advantage of being safe and easy to take. An extract of red bell pepper (Capsicum annuum L.) flesh markedly enhanced immunoglobulin M (IgM) production by murine B cells and their proliferation. The effect of the extract on IgM production was not abrogated by treatment with polymyxin B and was also observed in spleen cells of lipopolysaccharideunresponsive C3H/HeJ mice. Treatment of B cells with the extract resulted in an increase in the number of IgMsecreting cells and cluster of differentiation (CD)138-positive cells. The IgM production-enhancing activity was resistant to trypsin treatment but was heat-labile. The activity was eluted in a peak with a molecular weight of 6000 on gel filtration. These results demonstrated that the extract of red bell pepper enhanced IgM production in B cells and suggest that the active substance(s) in the extract is a non-proteinous heat-labile macromolecule.

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“…The ELISPOT assay was performed as described previously [18]. Briefly, each well of 96-well microtiter plates was coated with 50 L of goat anti-mouse IgM Ab (10 g/mL) by being incubated overnight at 4 • C and then washed three times with PBS containing 0.05% Tween-20.…”

Section: Enzyme-linked Immunospot (Elispot) Assay For Detection Of Pomentioning

confidence: 99%

Promotion of IL-4- and IL-5-dependent differentiation of anti-μ-primed B cells by ascorbic acid 2-glucoside

et al. 2009

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The stable ascorbic acid derivative 2-O-alpha-D-glucopyranosyl-L-ascorbic acid (AA-2G) was used to investigate the role of ascorbic acid (AA) in B cell differentiation in vitro. AA-2G is stable in a solution unlike AA but is hydrolyzed by cellular alpha-glucosidase to release AA. Mouse spleen B cells were primed for 2 days with an anti-mu antibody in the presence of interleukin (IL)-4 and IL-5 and then washed and recultured with AA-2G in the presence of IL-4 and IL-5. AA-2G, but not AA, dose-dependently increased IgM production, the greatest enhancement being 150% at concentrations of more than 0.5mM. In the absence of IL-4 and IL-5, primed B cells produced a negligible amount of IgM, and AA-2G had no effect. AA-2G-induced IgM production in the presence of IL-4 and IL-5 was inhibited by the alpha-glucosidase inhibitor castanospermine. Intracellular AA content, depleted during the priming period, increased by adding AA-2G at the start of reculture. Treatment of B cells with AA-2G resulted in an increase in the number of IgM-secreting cells, CD138-positive cells and CD45R/B220-negative cells. The number of viable cells in untreated cultures decreased gradually, but the decrease was significantly attenuated by AA-2G, resulting in about 70% more viable cells in AA-2G-treated cultures. AA-2G caused a slight but reproducible enhancement of DNA synthesis and a slight decrease in the number of cells with a sub-G1 DNA content. These results demonstrated that AA released from AA-2G enhanced cytokine-dependent IgM production in anti-mu-primed B cells and suggest that its effect is caused through promoting the differentiation of B cells to plasma cells and attenuating the gradual decrease in the number of viable cells.

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Differentiation of murine B cells induced by chondroitin sulfate B

Cited by 13 publications

References 40 publications

Dermatan Sulfate Interacts with Dead Cells and Regulates CD5+ B-Cell Fate

Dermatan Sulfate Interacts with Dead Cells and Regulates CD5+ B-Cell Fate

Enhancement of Immunoglobulin M Production in B Cells by the Extract of Red Bell Pepper

Promotion of IL-4- and IL-5-dependent differentiation of anti-μ-primed B cells by ascorbic acid 2-glucoside

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