Differentiation of NG108‐15 Neuroblastoma Cells by Serum Starvation or Dimethyl Sulfoxide Results in Marked Differences in Angiotensin II Receptor Subtype Expression

Seidman, Kimberly J.N; Barsuk, Jeffrey H.; Johnson, R. F.; Weyhenmeyer, James A.

doi:10.1046/j.1471-4159.1996.66031011.x

Cited by 32 publications

(18 citation statements)

References 23 publications

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“…Interestingly, we could not detect the expression of the C2 insert when Neuro-2a cells were induced to differentiate in the presence of retinoic acid or nerve growth factor. 3 Previously, Seidman et al (35) have shown that DMSO/low serum induced the expression of the angiotensin type 2 receptor, AT2, in neuroblastoma cells, NG108-15. The brain AT system has been shown to be involved in learning and memory as well as growth and development (53)(54)(55)(56).…”

Section: Discussionmentioning

confidence: 99%

“…To induce differentiation, cells were first cultured in growth medium for 24 h after seeding, and then growth medium was replaced with medium consisting of DMEM with 1.5% DMSO and 0.5% fetal bovine serum (differentiation medium (DM)) according to published protocols (35). For DNA/siRNA transfection, 1 g of plasmid DNA or 33 pmol of siRNA was transfected to 2 ϫ 10 5 undifferentiated Neuro-2a cells using Lipofectamine TM 2000 (Invitrogen).…”

Section: Identification and Quantification Of The C2 Insert In Mousementioning

confidence: 99%

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The Effect of Including the C2 Insert of Nonmuscle Myosin II-C on Neuritogenesis

Saha

Dey

Biswas

et al. 2013

Journal of Biological Chemistry

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Background:The functional importance of the C2 insert-containing isoform of nonmuscle myosin II-C is not known. Results: During the neuritogenesis of Neuro-2a cells, NM II-C1C2 becomes the predominant isoform of NM II-C. Decreasing NM II-C1C2 expression leads to shortening of neurites. Conclusion: NM II-C1C2 plays a role in the later stage of neuritogenesis. Significance: This work contributes to an understanding of the functional importance of NM II-C1C2.

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Section: Discussionmentioning

confidence: 99%

Section: Identification and Quantification Of The C2 Insert In Mousementioning

confidence: 99%

The Effect of Including the C2 Insert of Nonmuscle Myosin II-C on Neuritogenesis

Saha

Dey

Biswas

et al. 2013

Journal of Biological Chemistry

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“…[63] Differentiation of NG108-15 was induced by incubating the cells with the films in a serum-free culture medium. [64] By comparing these images with those of cells grown on nanotube films for 3 days in full medium ( Fig. 2), we notice remarkable changes in the morphology of the cells.…”

mentioning

confidence: 93%

Single‐Walled Carbon Nanotube Polyelectrolyte Multilayers and Freestanding Films as a Biocompatible Platform for Neuroprosthetic Implants

et al. 2005

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between 17 and 64 N m -1 , using scan rates of 1-2 lines s -1 and a resolution of 512 pixels × 512 pixels. The SFM images in Figure S2 were taken with an Autoprobe CP research microscope (Thermomicroscope, Veeco, Santa Barbara, California, USA) operating at room temperature in air. Images with scan lengths ranging from 5 lm down to 0.5 lm have been recorded with a resolution of 512 pixels × 512 pixels using the 5 lm scanner and non-contact Si ultralevers with a spring constant (k) in the range 2. [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17] The unique properties of single-walled carbon nanotubes (SWNTs) can lead to a new generation of composites with high strength, electrical conductivity, and chemical and structural stability.[1-10] These properties are being utilized in the fabrication of many proof-of-concept applications including nanoelectronic devices, [11][12][13] sensors, [14][15][16][17][18][19] field-emission electron sources, [20,21] displays and lighting elements, [22,23] and scanning probe tips. [24] Recent reports on the functionalization of nanotubes with bioactive groups such as peptides, [25][26][27][28] proteins, [29][30][31] and DNA [32,33] have also attracted attention to the potential importance of SWNTs in biology and medicine. So far, SWNTs have mainly been utilized as sensing materials for various biological molecules such as DNA, [34] glucose, [35][36][37] peptides, and proteins. [38][39][40][41] They have also been used as molecular tips functioning as biological probes.[

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“…For the experiments, confluent cultures were harvested with DMEM medium and the cells were seeded into 24-well or 96-well plates and grown for 24 h. Three different media combinations were used: standard (the normal growing medium described above), stress (glucoseand FCS-free) and differentiation medium (0.5% FCS/1.5% DMSO; (Seidman et al 1996).…”

Section: Methodsmentioning

confidence: 99%

Dual Neuroprotective and Neurotoxic Effects of Cannabinoid Drugs in Vitro

et al. 2010

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Either protective or toxic effects of cannabinoids on cell survival have been reported extensively in the literature; however, the factors that determine the direction of the effect are still obscured. In this study we have used the neuroblastoma cell line N18TG2 that expresses CB1 cannabinoid receptors to investigate several factors that may determine the consequences of exposure to cannabinoid agonists. Cells that were grown under optimal, stressful, or differentiating conditions were exposed to cannabinoid agonists and then assayed for cell viability by measuring MTT, LDH, and caspase-3 activity. Various cannabinoid agonists (CP 55,940, ∆9-THC, HU-210, and WIN 55,212-2) failed to affect cell viability when the cells were grown under optimal conditions. On the other hand, the same agonists significantly reduced cell viability when the cells were grown under stressful conditions (glucose- and serum-free medium), while enhancing the viability of cells grown in differentiation medium (0.5% serum and 1.5% DMSO). The toxic/protective profile was not dependent on the type or the concentration of the cannabinoid agonist that was applied. The cannabinoid agonist CP 55,940 similarly affected the non-neuronal HEK-293 cells that were grown under stressful conditions only when they expressed CB1 receptors. Our results shed light on the conflicting reports regarding the protective or toxic effects of cannabinoids in vitro and indicate that cannabinoids may activate different intracellular signaling mechanisms, depending on the state of the cell, thus leading to different physiological consequences.

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Differentiation of NG108‐15 Neuroblastoma Cells by Serum Starvation or Dimethyl Sulfoxide Results in Marked Differences in Angiotensin II Receptor Subtype Expression

Cited by 32 publications

References 23 publications

The Effect of Including the C2 Insert of Nonmuscle Myosin II-C on Neuritogenesis

The Effect of Including the C2 Insert of Nonmuscle Myosin II-C on Neuritogenesis

Single‐Walled Carbon Nanotube Polyelectrolyte Multilayers and Freestanding Films as a Biocompatible Platform for Neuroprosthetic Implants

Dual Neuroprotective and Neurotoxic Effects of Cannabinoid Drugs in Vitro

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