Differentiation of Oligodendrocyte Progenitor Cells from Human Embryonic Stem Cells on Vitronectin-Derived Synthetic Peptide Acrylate Surface

Li, Yan; Gautam, Archna; Yang, Jiwei; Qiu, Liqun; Melkoumian, Zara; Weber, Jennifer L.; Telukuntla, Lavanya; Srivastava, Rashi; Whiteley, Erik M.; Brandenberger, Ralph

doi:10.1089/scd.2012.0508

Cited by 44 publications

(43 citation statements)

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“…ECM is an important component during OPC differentiation, affecting both the differentiation efficiency and the derived cell function (Table 2). Thus, understanding the cell-ECM interactions and development of defined ECM substrates are critical steps for future clinical applications [13] .…”

Section: Cellsmentioning

confidence: 99%

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Neural differentiation from pluripotent stem cells: The role of natural and synthetic extracellular matrix

Liu

Yan

et al. 2014

WJSC

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Neural cells differentiated from pluripotent stem cells (PSCs), including both embryonic stem cells and induced pluripotent stem cells, provide a powerful tool for drug screening, disease modeling and regenerative medicine. High-purity oligodendrocyte progenitor cells (OPCs) and neural progenitor cells (NPCs) have been derived from PSCs recently due to the advancements in understanding the developmental signaling pathways. Extracellular matrices (ECM) have been shown to play important roles in regulating the survival, proliferation, and differentiation of neural cells. To improve the function and maturation of the derived neural cells from PSCs, understanding the effects of ECM over the course of neural differentiation of PSCs is critical. During neural differentiation of PSCs, the cells are sensitive to the properties of natural or synthetic ECMs, including biochemical composition, biomechanical properties, and structural/topographical features. This review summarizes recent advances in neural differentiation of human PSCs into OPCs and NPCs, focusing on the role of ECM in modulating the composition and function of the differentiated cells. Especially, the importance of using three-dimensional ECM scaffolds to simulate the in vivo microenvironment for neural differentiation of PSCs is highlighted. Future perspectives including the immediate applications of PSC-derived neural cells in drug screening and disease modeling are also discussed.© 2014 Baishideng Publishing Group Co., Limited. All rights reserved.Key words: Pluripotent stem cells; Neural differentiation; Extracellular matrix; Three-dimensional; Drug screening Core tip: Neural cells derived from human pluripotent stem cells (hPSCs), including oligodendrocyte progenitor cells and neural progenitor cells, emerge as an unlimited and physiologically relevant cell source for drug screening, disease modeling, and regenerative medicine. Natural and synthetic extracellular matrices play an important role in regulating neural differentiation, cell migration, and the derived neural cell maturation. Recent advances in neural differentiation of hPSCs on extracellular matrices in 2-D and 3-D systems are reviewed in this paper. The immediate applications of the derived neural cells in drug screening and disease modeling are also discussed.

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Section: Cellsmentioning

confidence: 99%

“…Differentiating hPSCs into OPCs or neural progenitor cells (NPCs) with a high purity has been demonstrated, but their function and maturation are still under investigation [12][13][14] . Extracellular matrix (ECM) plays an important role in neural differentiation of hPSCs and the maturation of the derived neural cells [15] .…”

Section: Introductionmentioning

confidence: 99%

Neural differentiation from pluripotent stem cells: The role of natural and synthetic extracellular matrix

Liu

Yan

et al. 2014

WJSC

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“…Therefore, using a biosensor to measure the in situ oxygen level, as shown in Figure 2, would be useful to fully understand the effect of oxygen in stem cell fate decision. Perfusion improved tissue architecture of engineered cardiac muscle and increased matrix synthesis in engineered chondrocytes [73,74] Agitation preserved Oct-4 expressing cells during PSC differentiation [82,83] for culturing and engineering stem cells [12,[34][35][36][37] . The material properties including surface topology and stiffness or tensile strength have profound effects on cell adhesion, proliferation, and the differentiated cellular function [38] .…”

Section: Control Of Oxygen Tension In Bioreactorsmentioning

confidence: 99%

Engineering stem cell niches in bioreactors

Liu

Liu²,

Zang³

et al. 2013

WJSC

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Stem cells, including embryonic stem cells, induced pluripotent stem cells, mesenchymal stem cells and amniotic fluid stem cells have the potential to be expanded and differentiated into various cell types in the body. Efficient differentiation of stem cells with the desired tissue-specific function is critical for stem cell-based cell therapy, tissue engineering, drug discovery and disease modeling. Bioreactors provide a great platform to regulate the stem cell microenvironment, known as "niches", to impact stem cell fate decision. The niche factors include the regulatory factors such as oxygen, extracellular matrix (synthetic and decellularized), paracrine/ autocrine signaling and physical forces (i.e. , mechanical force, electrical force and flow shear). The use of novel bioreactors with precise control and recapitulation of niche factors through modulating reactor operation parameters can enable efficient stem cell expansion and differentiation. Recently, the development of microfluidic devices and microbioreactors also provides powerful tools to manipulate the stem cell microenvironment by adjusting flow rate and cytokine gradients. In general, bioreactor engineering can be used to better modulate stem cell niches critical for stem cell expansion, differentiation and applications as novel cell-based biomedicines. This paper reviews important factors that can be more precisely controlled in bioreactors and their effects on stem cell engineering.

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“…23,28 To create the ECMs with lineage-specific cues, EBs were treated with retinoic acid (RA) to induce germlayer differentiation or BMP-4 for mesoderm differentiation. [31][32][33] To probe the role of RA signaling in the characteristics of decellularized ECMs, the cellular expression of a RA receptor (RAR) and the effect of a pan-RAR antagonist were investigated. The results suggest that the decellularized matrices derived from ESCs are able to preserve the developmental cues and have a distinct capacity in directing the ESC fate.…”

Section: Introductionmentioning

confidence: 99%

Extracellular Matrices Decellularized from Embryonic Stem Cells Maintained Their Structure and Signaling Specificity

Sart

2014

Tissue Engineering Part A

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Embryonic stem cells (ESCs) emerge as a promising tool for tissue engineering and regenerative medicines due to their extensive self-renewal ability and the capacity to give rise to cells from all three-germ layers. ESCs also secrete a large amount of endogenous extracellular matrices (ECMs), which play an important role in regulating ESC self-renewal, lineage commitment, and tissue morphogenesis. ECMs derived from ESCs have a broader signaling capacity compared to somatic ECMs and are predicted to have a lower risk of tumor formation associated with ESCs. In this study, ECMs from undifferentiated ESC monolayers, undifferentiated aggregates, or differentiated embryoid bodies at different developmental stages and lineage specifications were decellularized and their capacities to direct ESC proliferation and differentiation were characterized. The results demonstrate that the ESC-derived ECMs were able to influence ESC proliferation and differentiation by direct interactions with the cells and by influencing the signaling functions of the regulatory macromolecules such as retinoic acid. Such matrices have the potential to present regulatory signals to direct lineage-and developmentspecific cellular responses for in vitro applications or cell delivery.

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Differentiation of Oligodendrocyte Progenitor Cells from Human Embryonic Stem Cells on Vitronectin-Derived Synthetic Peptide Acrylate Surface

Cited by 44 publications

References 47 publications

Neural differentiation from pluripotent stem cells: The role of natural and synthetic extracellular matrix

Neural differentiation from pluripotent stem cells: The role of natural and synthetic extracellular matrix

Engineering stem cell niches in bioreactors

Extracellular Matrices Decellularized from Embryonic Stem Cells Maintained Their Structure and Signaling Specificity

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