Differentiation of oxidized low density lipoproteins by nanosensors

Rouhanizadeh, Mahsa; Tang, Tao; Li, Chao; Hwang, Juliana; Zhou, Chongwu; Hsiai, Tzung K.

doi:10.1016/j.snb.2005.06.067

Cited by 23 publications

(13 citation statements)

References 26 publications

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“…5). The random network geometry has several advantages: it eliminates the problems of nanotube alignment and assembly, eliminates conductivity variations due to nanotube chirality and geometry, and is tolerant to individual SWNT channel failure because the device characteristics are averaged over a large number of nanotubes (27). In addition, such devices can be developed on low-cost flexible and͞or Graph with electronic (1 Ϫ G͞G 0) responses in SNP detection assay (n ϭ 4, P ϭ 0.002) using 100 pM wt target in the presence of 5 g͞ml heat-denatured salmon DNA.…”

Section: Resultsmentioning

confidence: 99%

Label-free detection of DNA hybridization using carbon nanotube network field-effect transistors

Star

Niemann

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

662

540

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We report carbon nanotube network field-effect transistors (NTNFETs) that function as selective detectors of DNA immobilization and hybridization. NTNFETs with immobilized synthetic oligonucleotides have been shown to specifically recognize target DNA sequences, including H63D single-nucleotide polymorphism (SNP) discrimination in the HFE gene, responsible for hereditary hemochromatosis. The electronic responses of NTNFETs upon single-stranded DNA immobilization and subsequent DNA hybridization events were confirmed by using fluorescence-labeled oligonucleotides and then were further explored for label-free DNA detection at picomolar to micromolar concentrations. We have also observed a strong effect of DNA counterions on the electronic response, thus suggesting a charge-based mechanism of DNA detection using NTNFET devices. Implementation of label-free electronic detection assays using NTNFETs constitutes an important step toward low-cost, low-complexity, highly sensitive and accurate molecular diagnostics.hemochromatosis ͉ SNP ͉ biosensor T he development of nucleic acids diagnostics has become the subject of intense research, especially in the postgenome era. Current methods have mainly focused on optical detection using fluorescence-labeled oligonucleotides with dyes (1), quantum dots (2), or enhanced absorption of light by oligonucleotidemodified gold nanoparticles (3). On the other hand, label-free electronic methods promise to offer sensitivity, selectivity, and low cost for the detection of DNA hybridization (4). For example, microfabricated silicon field-effect sensors can monitor directly the increase in surface charge when DNA was hybridized on the sensor surface (5). Nanomaterials possess unique properties that are amenable to biosensor applications; they are one-dimensional structures that are extremely sensitive to electronic perturbations, readily functionalized with biorecognition layers, and compatible with many semiconducting manufacturing processes. Thus, one-dimensional silicon nanowires (6-8) and indium oxide nanowires (9) have shown promising performance, because their electronic conductance is more sensitive to DNA-associated charges as a result of their high surface-tovolume ratio. Using smaller nanowires with virtually all atoms on their surface, such as single-walled carbon nanotubes (SWNTs), will provide additional advantages in DNA detection. To date, there are several reports on electrochemical detection of DNA hybridization using multi-walled carbon nanotube electrodes (ref. 10 and references therein, and ref. 11). Whereas electrochemical methods rely on electrochemical behavior of the labels, measurement of direct electron transfer between SWNTs and DNA molecules paves the way for label-free DNA detection. SWNT-based field-effect transistors (12) have excellent operating characteristics (13), and they have already been explored for highly sensitive electronic detection of gases (14, 15) and biomolecules such as antibodies (16,17).Single-stranded DNA (ssDNA) has been recently demons...

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Section: Resultsmentioning

confidence: 99%

Label-free detection of DNA hybridization using carbon nanotube network field-effect transistors

Star

Niemann

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

662

540

View full text Add to dashboard Cite

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“…A porphyrinic nanosensor for in-situ measurement of nitric oxide in endothelial cells or in beating heart allowed understanding the effect of hypertension and ischemia/reperfusion on the release of NO [62]. In 2 O 3 nanowire-based FET sensors were employed as lab-on-a-chip devices for detection of oxidized low density lipoprotein (oxLDL) cholesterol [63] which is considered a biomarker for acute heart attack in patients with coronary artery disease (CAD).…”

Section: In Vivo Sensors For Cvdmentioning

confidence: 99%

Emerging applications of nanomedicine for the diagnosis and treatment of cardiovascular diseases

Godin

Sakamoto

Serda

et al. 2010

Trends in Pharmacological Sciences

218

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Nanomedicine is an emerging field of medicine which utilizes nanotechnology concepts for advanced therapy and diagnostics. This convergent discipline, which merges research areas such as chemistry, biology, physics, mathematics and engineering thus bridging the gap between molecular and cellular interactions, has a potential to revolutionize current medical practice. This review presents recent developments in nanomedicine research, which are poised to have an important impact on cardiovascular disease and treatment by improving therapy and diagnosis of such cardiovascular disorders as atherosclerosis, restenosis and myocardial infarction. Specifically, we discuss the use of nanoparticles for molecular imaging and advanced therapeutics, specially designed drug eluting stents and in vivo/ex vivo early detection techniques.

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“…Determination of LDL cholesterol is very important especially in individuals with a family history of coronary problems and carriers of ApoE 4 gene. Not only could LDL be detected using a nanosensor, but discrimination of an oxidized form of LDL and LDL was also effected using a indium oxide nanowire network and CNT network-based field-effect transistors (78). A selfassembling acetylcholine esterase on CNT-modified glassy carbon electrode (GCE) biosensor using amperometry for the detection of nerve agents and organophosphate pesticides was recently reported (79).…”

Section: Electrochemical-based Nanosensorsmentioning

confidence: 99%

The study of Alzheimer’s disease biomarkers

2006

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Alzheimer's disease (AD) is by far the most common dementing illness of late life and is increasing with the ever-growing number of older adults, in particular, in developed countries. The disease is often referred to as the "Long-Goodbye" because the person with the illness slowly becomes lost to everyone a long time before the body finally gives out. Being able to detect AD earlier on during the course of the disease offers better prospects for the future, for AD individuals, their families and friends as well as on the economy, as a whole. Unfortunately, such a detection technique is not yet, available. However, there are a number of promising biological markers (biomarkers) that correlate well with clinical cognitive tests of individuals and/or postmortem histopathological manifestations of the disease, especially when at least two markers are used for the diagnosis.Biosensors are tools that combine a biochemical binding element to a signal conversion unit and are already being used in the study of some AD biomarkers. However, their use in clinical diagnosis remains a challenge. Introduction of nanotechnology leading to nanobiosensors has several potential advantages over other clinical and/or existing analytical tools, including increased assay speed, flexibility, reduced cost of diagnostic testing, potential to deliver molecular diagnostic tools to family general practitioners, and other health care systems. Even more important, nano-based assays have the potential to detect target proteins at attomolar concentration level. They are, therefore, being increasingly exploited for the detection of early metabolic changes associated with diseases. Because brain damage is irreversible, the use of nanotechnology is particularly important in AD and other neurodegenerative disorders. Nanosensors can also facilitate and enable pointof-care-testing (POCT). This article reviews the basic biochemical processes that lead to AD pathology, current biomarkers for AD, and the current role of nanosensor technology for the study of AD biomarkers. Furthermore, it discusses the huge potential of nanosensing to deliver new molecular diagnostic strategies to AD research.(Nanobiotechnology

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Differentiation of oxidized low density lipoproteins by nanosensors

Cited by 23 publications

References 26 publications

Label-free detection of DNA hybridization using carbon nanotube network field-effect transistors

Label-free detection of DNA hybridization using carbon nanotube network field-effect transistors

Emerging applications of nanomedicine for the diagnosis and treatment of cardiovascular diseases

The study of Alzheimer’s disease biomarkers

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