2010
DOI: 10.1053/j.gastro.2010.02.011
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Differentiation of Pancreatic Acinar Cells to Hepatocytes Requires an Intermediate Cell Type

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Cited by 19 publications
(20 citation statements)
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“…This could be a C. elegans-specific feature: indeed, nuclear reprogramming from one cell type-specific gene expression programme to another could be a comparatively simple process in C. elegans because, for example, they lack DNA methylation, which impacts on gene expression (Bird, 2002). However, even though direct evidence is missing, BrdU staining during various cell type conversions in mammals has suggested that they too occur in the absence of cell division (Means et al, 2005;Wu et al, 2010;Zhou et al, 2008), indicating that the complexity of DNA or chromatin modifications is not an obstacle to nuclear reprogramming in the absence of cell division. Consistently, recent findings have shown that elevated cell division does not accelerate iPS derivation by increasing the overall efficiency of the process itself, but rather by increasing the probability of a stochastic reprogramming event (Hanna et al, 2009).…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…This could be a C. elegans-specific feature: indeed, nuclear reprogramming from one cell type-specific gene expression programme to another could be a comparatively simple process in C. elegans because, for example, they lack DNA methylation, which impacts on gene expression (Bird, 2002). However, even though direct evidence is missing, BrdU staining during various cell type conversions in mammals has suggested that they too occur in the absence of cell division (Means et al, 2005;Wu et al, 2010;Zhou et al, 2008), indicating that the complexity of DNA or chromatin modifications is not an obstacle to nuclear reprogramming in the absence of cell division. Consistently, recent findings have shown that elevated cell division does not accelerate iPS derivation by increasing the overall efficiency of the process itself, but rather by increasing the probability of a stochastic reprogramming event (Hanna et al, 2009).…”
Section: Discussionmentioning
confidence: 99%
“…In mammals, although the detailed sequence is missing, direct cell type conversion of pancreatic acinar to ductal cells has been suspected to occur through an intermediary cellular stage (Doetsch, 2003;Means et al, 2005;Pastrana et al, 2009;Wu et al, 2010). Similarly, pancreatic cell-to-hepatocyte conversion, the formation of coronary arteries from venous cells and the conversion of astrocytes into neuroblasts during adult neurogenesis are also suspected to occur through an intermediate cell that is less differentiated (Doetsch, 2003;Means et al, 2005;Pastrana et al, 2009;Red-Horse et al, 2010;Wu et al, 2010). Thus, some features of the Y-to-PDA conversion highlighted in this study are most likely relevant to other organisms.…”
Section: Discussionmentioning
confidence: 99%
“…Elas-CreER transgenic mice were generated at the Level Transgenic Center (Taipei, Taiwan) as described in as our previous work [43]. Kras +/LSLG12D mice (B6;129-Kras2 <tm4Tyj>) bearing a Cre-dependent conditional knock-in mutation Kras G12D were imported from Mouse Models of Human Cancer Consortium [44].…”
Section: Methodsmentioning
confidence: 99%
“…Pancreata harvested from 14-wk-old elastase-CreERKras G12D mice (elastase-CreER ϫ LSL-Kras G12D ) (42,44) were used to develop 3D duct lesion histology. To examine early stage duct lesion formation, elastase-CreER-Kras G12D mice were injected with tamoxifen (Sigma, St. Louis, MO) at age 6 wk (2 mg/injection, three injections in 1 wk to induce Cre-mediated recombination), and duct lesion was allowed to develop for the next 7 wk.…”
Section: Methodsmentioning
confidence: 99%