Differentiation of Plasma Antithrombin Activities.

Fell, Colin; Ivanovic, Nevenka; Johnson, Shirley A.; Seegers, Walter H.

doi:10.3181/00379727-85-20829

Cited by 42 publications

(17 citation statements)

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“…The reason for this activation may be that plasminogen is converted into plasmin by means of plasminogen activators. Plasmin activates the degradation of fibrin into FDP; these will react as antithrombin VI and inhibit the effect of factor IIa (thrombin) (Fell et al 1954).…”

Section: <20%mentioning

confidence: 99%

Haemostatic and fibrinolytic properties of peritoneal fluid in the menstrual cycle

Bouckaert¹,

Wersch²,

Schellekens³

et al. 1984

BJOG

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The haemostatic and fibrinolytic properties of peritoneal fluid aspirated during laparoscopy were studied in a group of 49 women, 12 patients during the follicular phase of the menstrual cycle, and 37 during the luteal phase. Haemoglobin concentration and platelet count were low and similar in the pre-and post-ovulatory groups. Factor VIII was present in low concentrations both in the pre-and post-ovulatory period. The fibrinolytic and antifibrinolytic activity as judged by measurements of plasminogen, a,-antiplasmin, fibrinogen degradation products, fibrinogen and antithrombin 111, rose significantly in the postovulatory period. It is concluded that the fibrinolytic activity of the peritoneal fluid depends on the stage of the menstrual cycle and is higher during the luteal phase.

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Section: <20%mentioning

confidence: 99%

Haemostatic and fibrinolytic properties of peritoneal fluid in the menstrual cycle

Bouckaert¹,

Wersch²,

Schellekens³

et al. 1984

BJOG

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“…The adsorption of thrombin on fibrinogen is referred to as antithrombin I (Fell et al, 1954). Thus the presence of fibrinogen in a plasma saiiiple greatly affects the results of antithrombin assays carried out using coagulation tests.…”

Section: Efect Of Fihrinogeii On Antithrombin Estimation Nsing the Tamentioning

confidence: 99%

A New Assay for the Measurement of Total Progressive Antithrombin

Lane¹,

Bird²,

Rizza³

1975

Thrombosis and Haemostasis VTH Congress

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A new rapid method for assaying total antithrombin activity has bcen developed based on the inactivation of thrombin incorporated into an agarose gel, during the radial diffusion of plasma in the gel. The area of thrombin inactivation is subsequently observed by the coagulation of fibrinogen in a separate agarose gel layer poured over the thrombin gel. The method is describcd in dctail and its accuracy assessed with respect to other antithrombin assays. Using specific antiscra to a,-globulin (antithrombin III), a,-macroglobulin and a -antitrypsin, total antithrombin activity measured by this assay consisted of 47% a,-globulin, 29% a,-macroglobulin and 26% a1 -antitrypsin.Various clotting techniques have been used to estimate the amount of progrcssivc antithrombin activity (QuickYue et al, 1973). Thc mcthod of Biggs ct a1 (1970) which is used in this laboratory is time consuming and has the disadvantage that the plasma must be defibrinated before it is tested. In addition to clotting methods, scveral differciit diffusion methods have been used to estimate the amount and study the properties of antithrombin. Ganrot (1969) studied the identities of antithrombin 111 and heparin cofactor (antithrombin 11) by electrophoresis of plasma through an agarose gel containing fibrinogcn, then spraying the gcl with thrombin, or heparin then thrombin, and observing for areas in which fibrin was not formed, attributing this to either antithrombin I1 or antithrombin 111 . Heimburger (1967, 1972), Heimburger et al (1970) and Heimburger & Trobisch (1971) have used the principlc of lysis by proteolytic enzymes of a fibrin agar plate, through which plasma has been electrophorcsed, to study the properties of antithrombin I1 and antithrombin III. The principle of radial diffusion of plasma through an agarose gel containing thrombin and subsequent usc of fibrinogen to detect areas of thrombin inactivation has been used by us to develop a niethod for the measurement of total progrcssive antithrombin activity. It is based on a method developed by Bird (1975) to measure antibodies to factor VIII by plasma coagulation in an agarose gel. This antithrombin method is less time consuming than the conventional coagulation techniques and does not require plasma defibrination.Total progressive antithrombin activity has been attributed to three globulins, a,-2% agarose gel in 0.85% w/v saline, and 0.6% w/v agarose gel in distilled water were prepared by boiling in a water bath for 20 min. The gel may be stored in aliquots at 4°C for periods of up to I week.Rabbit antibodies (Behringwerke) to a,-antitrypsiii and a,-macroglobulin were obtained from Hoechst Pharmaceuticals, Hounslow, Middlesex, England. Rabbit antibody (Nyegaard) to antithrombin I11 (!x2-globulin) was obtained from BDH Chcmicals Ltd, Poole, Dorset,England. The specificity of each antiserum was determined by immunoelectrophoresis in 1% agarose gel at pH 8.6 using normal human plasma as the source of antigen (Grabar & , 1953). A single and characteristic precipitation line was observed w...

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“….Is already mentioned, the heparin cofactor activity is not destroyed Iiy ether extraction. It can be precipitated from diluted plasma with inow (NH&304 than is required to precipitate antithrombin-I11 (76).…”

Section: Antithrombin-i1mentioning

confidence: 99%

Coagulation of the Blood

Seegers

1955

Advances in Enzymology - And Related Areas of Molecular Biology

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Differentiation of Plasma Antithrombin Activities.

Cited by 42 publications

References 0 publications

Haemostatic and fibrinolytic properties of peritoneal fluid in the menstrual cycle

Haemostatic and fibrinolytic properties of peritoneal fluid in the menstrual cycle

A New Assay for the Measurement of Total Progressive Antithrombin

Coagulation of the Blood

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