Differentiation of pluripotent stem cells into endothelial cells

Yoder, Mervin C.

doi:10.1097/moh.0000000000000140

Cited by 59 publications

(37 citation statements)

References 27 publications

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“…Growing interest in the isolation of EC/EPC from PSC can be observed, emerging from important therapeutic implications (Yoder, 2015). Successful methods for the differentiation of EC from embryonic stem cells (ESC) are broadly described in the literature, yet they mostly rely on the use of FBS (Levenberg, Golub, Amit, Itskovitz-Eldor, & Langer, 2002;McCloskey, Stice, & Nerem, 2006).…”

Section: Epc Expansion In Sf and CD Mediamentioning

confidence: 99%

Fetal bovine serum-free culture of endothelial progenitor cells-progress and challenges

Bauman

Granja

Barrias

2018

J Tissue Eng Regen Med

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Two decades after the first report on endothelial progenitor cells (EPC), their key role in postnatal vasculogenesis and vascular repair is well established. The therapeutic potential of EPC and their growing use in clinical trials calls for the development of more robust, reproducible, and safer methods for the in vitro expansion and maintenance of these cells. Despite many limitations associated with its usage, fetal bovine serum (FBS) is still widely applied as a cell culture supplement. Although different approaches aiming at establishing FBS-free culture have been developed for many cell types, adequate solutions for endothelial cells, and for EPC in particular, are still scarce, possibly due to the multiple challenges that have to be faced when culturing these cells. In this review, we provide a brief overview on the therapeutic relevance of EPC and critically analyse the available literature on FBS-free endothelial cell culture methods, including xeno-free, serum-free, and chemically defined systems.

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Section: Epc Expansion In Sf and CD Mediamentioning

confidence: 99%

Fetal bovine serum-free culture of endothelial progenitor cells-progress and challenges

Bauman

Granja

Barrias

2018

J Tissue Eng Regen Med

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“…Another approach is to supplement the cell population at the amputation plane by isolation of select cells from the injury site or elsewhere, expansion of those subpopulations ex vivo, and then redelivery of the proper phenotypes for regeneration, a paradigm similar to that used in other regenerative applications. 122 124 and FGF 125 have been found to be potent signals in stem cells to promote differentiation to the osteo, endothelial, and neural phenotypes, respectively. The goal of those studies, however, is to promote a single target phenotype.…”

Section: Translating Epimorphic Regenerationmentioning

confidence: 99%

Looking Ahead to Engineering Epimorphic Regeneration of a Human Digit or Limb

Quijano

Lynch

Allan

et al. 2016

Tissue Engineering Part B: Reviews

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Approximately 2 million people have had limb amputations in the United States due to disease or injury, with more than 185,000 new amputations every year. The ability to promote epimorphic regeneration, or the regrowth of a biologically based digit or limb, would radically change the prognosis for amputees. This ambitious goal includes the regrowth of a large number of tissues that need to be properly assembled and patterned to create a fully functional structure. We have yet to even identify, let alone address, all the obstacles along the extended progression that limit epimorphic regeneration in humans. This review aims to present introductory fundamentals in epimorphic regeneration to facilitate design and conduct of research from a tissue engineering and regenerative medicine perspective. We describe the clinical scenario of human digit healing, featuring published reports of regenerative potential. We then broadly delineate the processes of epimorphic regeneration in nonmammalian systems and describe a few mammalian regeneration models. We give particular focus to the murine digit tip, which allows for comparative studies of regeneration-competent and regeneration-incompetent outcomes in the same animal. Finally, we describe a few forward-thinking opportunities for promoting epimorphic regeneration in humans.

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“…65 In addition to matrices, designing the appropriate vasculature may become a reality using PSC-derived endothelial cells in which the identification of distinct endothelial lineages have already been described. 86,87 Soluble triggers of PPF and platelet release have not been identified but putative mediators include SDF-1 and sphingosine-1-phosphate. The SDF-1/CXC chemokine receptor type 4 signaling axis has been shown to be involved in the migration and location of megakaryocytes to appropriate bone marrow niche locations for platelet release.…”

Section: Megakaryocyte Maturation Ppf and Platelet Releasementioning

confidence: 99%

Understanding platelet generation from megakaryocytes: implications for in vitro–derived platelets

Sim

Poncz

Gadue

et al. 2016

Blood

105

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Platelets are anucleate cytoplasmic discs derived from megakaryocytes that circulate in the blood and have major roles in hemostasis, thrombosis, inflammation, and vascular biology. Platelet transfusions are required to prevent the potentially life-threatening complications of severe thrombocytopenia seen in a variety of medical settings including cancer therapy, trauma, and sepsis. Platelets used in the clinic are currently donor-derived which is associated with concerns over sufficient availability, quality, and complications due to immunologic and/or infectious issues. To overcome our dependence on donor-derived platelets for transfusion, efforts have been made to generate in vitro–based platelets. Work in this area has advanced our understanding of the complex processes that megakaryocytes must undergo to generate platelets both in vivo and in vitro. This knowledge has also defined the challenges that must be overcome to bring in vitro–based platelet manufacturing to a clinical reality. This review will focus on our understanding of committed megakaryocytes and platelet release in vivo and in vitro, and how this knowledge can guide the development of in vitro–derived platelets for clinical application.

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Differentiation of pluripotent stem cells into endothelial cells

Cited by 59 publications

References 27 publications

Fetal bovine serum-free culture of endothelial progenitor cells-progress and challenges

Fetal bovine serum-free culture of endothelial progenitor cells-progress and challenges

Looking Ahead to Engineering Epimorphic Regeneration of a Human Digit or Limb

Understanding platelet generation from megakaryocytes: implications for in vitro–derived platelets

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