Differentiation of pre-osteoblast cells on poly(ethylene terephthalate) grafted with RGD and/or BMPs mimetic peptides

Zouani, Omar F.; Chollet, Catherine; Guillotin, Bertrand; Durrieu, Marie‐Christine

doi:10.1016/j.biomaterials.2010.07.042

Cited by 114 publications

(137 citation statements)

References 56 publications

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“…Furthermore, in vitro models may not show complex cellular interaction between inflammatory or other adjacent cells that may affect release characteristics and inactivation of the BMP-7 from the surface. Based on our studies (data not shown) and literature, 20 the functionalized peptide showed osteogenesis under osteogenic media induction. As the peptide is securely bound to the substrates via covalent bioconjugation, there is no release of the peptide to the culture medium.…”

Section: Discussionmentioning

confidence: 76%

“…The pictures were transformed into binary images by setting a threshold whereby the threshold values were determined empirically. 20 Tissue Culture Passage five mouse fibroblasts (NIH/3T3) were routinely cultured in 10% Calf Serum and 5% penicillin/streptomycin in DMEM. 25 Fibroblasts were grown at 378C in a moist atmosphere of 5% CO 2 ; growth media was changed every couple of days.…”

Section: Determination Of Bioconjugationmentioning

confidence: 99%

“…More economical BMP peptides with osteogenic properties were developed due to the exorbitant cost of growth factors. 19,20 BMP-7 peptide developed from the knuckle epitope of BMP-7 growth factor 20 was selected for this study because it activates BMP-7 signaling via vibration of the BMP receptor II. To this BMP-7 peptide, 20 a cysteine amino acid was attached at the N-terminal end, and a minipolyethylene glycol (miniPEG) was added as a spacer.…”

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confidence: 99%

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Anti‐fibrosis effect of BMP‐7 peptide functionalization on cobalt chromium alloy

Tan

Poh

Cai

et al. 2013

Journal Orthopaedic Research

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Orthopedic metallic prosthetic implants are commonly made of cobalt chromium (CoCr) alloys. However, such metal-based implants are susceptible to fibrous capsule formation on the implant surface after implantation. At the bone-implant interface, this capsule can prevent implant integration, resulting in loosening and failure. Minimizing the development of such a capsule on the CoCr surface would improve direct bone-implant bonding leading to long-term implant functionality. We evaluated the anti-fibrosis effect of bone morphogenic protein-7 (BMP-7) peptide covalently bonded to CoCr alloy. This peptide, a biomimetic derivation of the knuckle epitope of BMP-7, was conjugated at the N-terminus with a cysteine amino acid. X-ray photoelectron spectroscopy (XPS) and probe binding assay were used to evaluate different stages of grafting and surface functionalization using polydopamine coating. Cellular functions were studied using fibroblast attachment, cell proliferation, and MTT assays. Fibroblasts were grown on functionalized and pristine CoCr substrates, and the efficacy of BMP-7 peptide on anti-fibrosis was analyzed via gene expression and protein expression of fibrosis markers ACTA2, Collagen 1A1, and fibronectin. The peptide functionalized substrates showed significant reduction of fibrosis markers expression after 1 week of incubation compared to controls. BMP-7 signaling pathway activation was shown by the presence of phosphorylation of Smad1/5/8. These findings may contribute to the improvement of CoCr implants in orthopedic surgery applications.

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Section: Discussionmentioning

confidence: 76%

Section: Determination Of Bioconjugationmentioning

confidence: 99%

mentioning

confidence: 99%

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Anti‐fibrosis effect of BMP‐7 peptide functionalization on cobalt chromium alloy

Tan

Poh

Cai

et al. 2013

Journal Orthopaedic Research

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“…However, the RGD motif and its derivatives are not the only integrin-binding sequence used for scaffold modification. For example, polyethylene terephthalate surfaces modified with the cell adhesion motif GRGDSPC showed improved differentiation of pre-osteoblastic cells as compared to nongrafted surfaces (Zouani et al, 2010). Alginate hydrogels modified with G 4 RGDY were able to support the formation of growth-plate-like structures in a co-culture of mouse osteoblasts and chondrocytes (Alsberg et al, 2002).…”

Section: Fibronectin and Small Cell Adhesion Motifsmentioning

confidence: 99%

Bioactive Scaffolds for the Controlled Formation of Complex Skeletal Tissues

Hofmann

García-Fuentes²

2011

Regenerative Medicine and Tissue Engineering - Cells and Biomaterials

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“…Several studies 127,[136][137][138] have shown that the culture of osteoblast precursor cells on substrates modified with immobilized BMP-2 can significantly increase the expression of osteogenic differentiation markers. These immobilization techniques can even be combined with patterning approaches.…”

Section: 57mentioning

confidence: 99%

Controlled Release Strategies for Bone, Cartilage, and Osteochondral Engineering—Part II: Challenges on the Evolution from Single to Multiple Bioactive Factor Delivery

Santo

Gomes²,

Mano³

et al. 2013

Tissue Engineering Part B: Reviews

105

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The development of controlled release systems for the regeneration of bone, cartilage, and osteochondral interface is one of the hot topics in the field of tissue engineering and regenerative medicine. However, the majority of the developed systems consider only the release of a single growth factor, which is a limiting step for the success of the therapy. More recent studies have been focused on the design and tailoring of appropriate combinations of bioactive factors to match the desired goals regarding tissue regeneration. In fact, considering the complexity of extracellular matrix and the diversity of growth factors and cytokines involved in each biological response, it is expected that an appropriate combination of bioactive factors could lead to more successful outcomes in tissue regeneration. In this review, the evolution on the development of dual and multiple bioactive factor release systems for bone, cartilage, and osteochondral interface is overviewed, specifically the relevance of parameters such as dosage and spatiotemporal distribution of bioactive factors. A comprehensive collection of studies focused on the delivery of bioactive factors is also presented while highlighting the increasing impact of platelet-rich plasma as an autologous source of multiple growth factors.

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Differentiation of pre-osteoblast cells on poly(ethylene terephthalate) grafted with RGD and/or BMPs mimetic peptides

Cited by 114 publications

References 56 publications

Anti‐fibrosis effect of BMP‐7 peptide functionalization on cobalt chromium alloy

Anti‐fibrosis effect of BMP‐7 peptide functionalization on cobalt chromium alloy

Bioactive Scaffolds for the Controlled Formation of Complex Skeletal Tissues

Controlled Release Strategies for Bone, Cartilage, and Osteochondral Engineering—Part II: Challenges on the Evolution from Single to Multiple Bioactive Factor Delivery

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