Differentiation of Salmonella Typhimurium from Salmonella Enteritidis and other Salmonella Serotypes using Random Amplified Polymorphic DNA Analysis

Gürakan, G. Candan; Aksoy, Canan; Ögel, Zümrüt B.; Oren, N G

doi:10.3382/ps.2007-00355

Cited by 10 publications

(13 citation statements)

References 27 publications

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“…Thus, the three reference strains yielded different band profiles with this primer. This observation agrees with those of McKenna et al (1995) on the differentiation of Ty21a vaccine strain from the rest of Salmonella Typhi isolates using a single arbitrary primer, and those reported by Gürakan et al (2008) on the use of a single RAPD primer as a serotype-specific marker for Salmonella Typhimurium.…”

Section: Resultssupporting

confidence: 93%

Typing of Salmonella Typhi strains isolated from Egypt by RAPD PCR

Rezk¹,

Mansour

Ghoneim

et al. 2011

3 Biotech

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PCR-based fingerprinting using random amplified polymorphic DNA (RAPD) has been used widely for genome identification. In this study, 13 Salmonella Typhi strains were isolated from typhoid patients from Aswan, Cairo, Fayoum, and Monofya Governorates of Egypt. The isolates, along with three reference strains, i.e., O901, H901, and Ty2 were subjected to whole genome typing by RAPD PCR. Three RAPD-PCR 10-mer primers generated a total of 85 RAPD bands (81 polymorphic bands), 12 distinct PCR profiles, and proved to be useful for discriminating the isolates and strains studied. Interestingly, the B1 and C1 PCR profile were found only in Cairo and Monofya, respectively; and some PCR types appeared only in certain Governorates of Egypt. By combining the profiles obtained with the primer trio used in this study, an excellent discrimination index (D) of 0.942 was reached. Pairwise comparisons of Jaccard’s similarity coefficients calculated among the 12 PCR types identified three major clusters; i.e., O901 branch and Ty2 and H901 sub-branches. Principal component analysis adequately resolved each of these three major clusters. Three principal components accounted for about 72% of the variation, with the first two components accounting for about 62% of the total variance among the genotypes studied. Biclustering improved the display of groups of RAPD amplicons (markers) that cluster similarly across the genomes and could delineate features pertaining to genome structure. In conclusion, RAPD PCR provided a fast method with high potentials in surveillance and epidemiological investigations of Salmonella Typhi infections.

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Section: Resultssupporting

confidence: 93%

Typing of Salmonella Typhi strains isolated from Egypt by RAPD PCR

Rezk¹,

Mansour

Ghoneim

et al. 2011

3 Biotech

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“…While the fatty acids in the OS essential oil generally contains polymorphic compounds, such as thymol and carvacrol, compounds, such as γ-terpinene, P-cymene, and thymoquinene, can also be found depending on the growing area (Lukas et al, 2009) . Some studies have reported that essential fatty acids in OS have antioxidant (Luna et al, 2010;Tavarez et al, 2011), anti-microbial (Mitsch et al, 2004;Gürakan et al, 2008), anti-inflammatory (Yoshino et al, 2006), anti-viral (Pilau et al, 2011), anti-tumoral (El-desoukya et al, 2009Al-kalaldeh et al, 2010), anti-ulcer (Afify et al, 2012), anti-fungal (Manohar et al, 2001), and anti-parasitic (Değerli et al, 2012;Remmal et al, 2013) properties, all of which have positive effects on stock performance (Jang et al, 2007;Tekçe & Gül, 2016).…”

Section: Effects Of Origanum Syriacum Essential Oil On Blood Parametementioning

confidence: 99%

Effects of Origanum Syriacum Essential Oil on Blood Parameters of Broilers Reared at High Ambient Heat

Tekce

Gül

2017

Rev. Bras. Cienc. Avic.

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“…Salmonella spp are among the group of bacteria in the family Enterobacteriaceae commonly known as enteric bacteria that live in gastrointestinal tracts of warm-blooded animals [3]. The genus Salmonella comprises about 2,579 serovars [8].…”

Section: Background On Salmonella Spp and Salmonellosismentioning

confidence: 99%

“…Salmonella spp are also responsible for typhoid in human in which an estimated 12-33 million cases of typhoid fever occurs globally each year and mortality rate of 10% to 30% reported in Asia and Africa respectively each year [3]. Specific Salmonella spp which are responsible for typhoid in humans are Salmonella typhi and Salmonella paratyphi A and B [2,3].…”

Section: Background On Salmonella Spp and Salmonellosismentioning

confidence: 99%

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Review on the Problematic of Salmonellosis and Interests of Traditional Herbs in the Treatment

Dougnon¹,

Bankolè²,

Johnson³

et al. 2016

Clin Microbiol

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Objective: Salmonellae are facultative anaerobic Gram-negative rod-shaped bacteria generally 2-5 microns long by 0.5-1.5 microns wide and motile by peritrichous flagella. Salmonella spp are among the group of bacteria in the family Enterobacteriaceae commonly known as enteric bacteria that live in gastrointestinal tracts of warm-blooded animals. Worldwide surveillance data has demonstrated an overall increase in antibiotic resistance among nontyphoid Salmonella, although significant geographical and serotype variability exist. The use of medicinal plants in the treatment of salmonellosis has become more than compulsory nowadays especially with the new trend of antibiotic resistance and the effectiveness of the properties of these plants. The current study aimed to assess the importance of salmonellosis in Benin and the potentialities of treatment by medicinal plants. Materials and Methods:A systematic online search was performed whereby key-words such as "Salmonellae", "Salmonellosis Benin", "non-typhoidal salmonellosis", "Medicinal plants Benin" and "traditional plants Benin" were entered in PubMed database, Google Scholar and www.google.bj. Obtained articles were included based on the reliability of their source, the study area (basically Benin and Africa) and the topic. Results:Host factors predisposing to severe non-typhoidal Salmonellae infection include reduced gastric acidity, impaired cell mediated and humoral immunity, and impaired phagocytic function. Antimicrobial resistance in Salmonella can be associated with horizontal transfer of antibiotic resistant genes and with increased treatment failure and risk of invasive disease. Several scientists around the world have researched this matter and confirmed the antimicrobial properties of plants on bacterial infections mainly salmonellosis Conclusion:The use of medicinal plants in the treatment of salmonellosis has become more than compulsory nowadays especially with the new trend of antibiotic resistance and the effectiveness of the properties of these plants.

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Differentiation of Salmonella Typhimurium from Salmonella Enteritidis and other Salmonella Serotypes using Random Amplified Polymorphic DNA Analysis

Cited by 10 publications

References 27 publications

Typing of Salmonella Typhi strains isolated from Egypt by RAPD PCR

Typing of Salmonella Typhi strains isolated from Egypt by RAPD PCR

Effects of Origanum Syriacum Essential Oil on Blood Parameters of Broilers Reared at High Ambient Heat

Review on the Problematic of Salmonellosis and Interests of Traditional Herbs in the Treatment

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