Differentiation of seminiferous tubule-associated stem cells into leydig cell and myoid cell lineages

Zhao, Xingxing; Wen, Xin; Ji, Minpeng; Guan, Xiaoju; Chen, Panpan; Hao, Xinrui; Chen, Fen-Fen; Hu, Yue; Duan, Peng; Ge, Ren Shan; Chen, Haolin

doi:10.1016/j.mce.2021.111179

Cited by 23 publications

(13 citation statements)

References 31 publications

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“…In support of this possibility, Leydig cells in Older Group2 show higher expression of the Leydig/TPC progenitor cell markers, alongside lower expression of mature Leydig cell markers. We also observe clear reductions in HH receptors in aging Leydig cells and the weakened HH signaling interaction of Sertoli-Leydig cells; results in keeping with prior in vitro studies that HH signaling induces the common progenitor cells to differentiate into Leydig cells in rats (Zhao et al, 2021). Third, we observe TPC hyperplasia in aging testes-which is associated with less contractility and abnormal secretion of basement membrane components, factors that could impair the transport of sperm along the tubule as well as disrupting interactions between SSCs and the basement membrane.…”

Section: Aging Men With Elevated Bmi Are Molecularly and Phenotypical...supporting

confidence: 91%

Single-cell analysis of human testis aging and correlation with elevated body mass index

et al. 2022

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Section: Aging Men With Elevated Bmi Are Molecularly and Phenotypical...supporting

confidence: 91%

Single-cell analysis of human testis aging and correlation with elevated body mass index

et al. 2022

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“…A number of markers for LSCs had been previously suggested in human and nonhuman species. They include THY1 (CD90), CD51 (ITGAV), COUP-TFII (NR2F2), PDGFRA, TCF21 and Endoglein (ENG) [ 46 , 47 ]. These were readily detected, yet Nestin ( NES ) and ARX were not readily found.…”

Section: Resultsmentioning

confidence: 99%

The Molecular Signature of Human Testicular Peritubular Cells Revealed by Single-Cell Analysis

Liebich

Schmid

Koupourtidou

et al. 2022

Cells

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Peritubular cells of the human testis form a small compartment surrounding the seminiferous tubules. They are crucial for sperm transport, and they emerge as contributors to the spermatogonial stem cell niche. They are among the least known cell types of the human body. We employed single-cell RNA sequencing of cultured human testicular peritubular cells (HTPCs), which had been isolated from testicular samples of donors with normal spermatogenesis. The significant overlap between our results and recently published ex vivo data indicates that HTPCs are a highly adequate cellular model to define and study these cells. Thus, based on the expression of several markers, HTPCs can be classified as testicular smooth muscle cells. Small differences between the in vivo/in vitro expressed genes may be due to cellular plasticity. Plasticity was also shown upon addition of FCS to the culture medium. Based on transcriptome similarities, four cellular states were identified. Further analyses confirmed the presence of known stem cell niche-relevant factors (e.g., GDNF) and identified unknown functions, e.g., the ability to produce retinoic acid. Therefore, HTPCs allow us to define the signature(s) and delineate the functions of human testicular peritubular cells. The data may also serve as a resource for future studies to better understand male (in)fertility.

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“…However, we showed a significant reduction in the number of Leydig cells in adult testes only after fetal RAD exposure alone, although there was no decrease in the Leydig cell number in the neonatal testes. Adult Leydig cells arise from multiple progenitors, such as fetal Leydig cells, peritubular myoid cells and vascular pericytes, during puberty [54,55]. Analysis of the expression of distinct markers of adult and fetal Leydig cells such as Hsd3b6 or Cyp26b1, respectively [56,57], in adult testes suggests a common dysfunction of adult and "fetal" Leydig cells.…”

Section: Discussionmentioning

confidence: 99%

Unexpected Interacting Effects of Physical (Radiation) and Chemical (Bisphenol A) Treatments on Male Reproductive Functions in Mice

Wieckowski

Ranga

Moison

et al. 2021

IJMS

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For decades, numerous chemical pollutants have been described to interfere with endogenous hormone metabolism/signaling altering reproductive functions. Among these endocrine disrupting substances, Bisphenol A (BPA), a widely used compound, is known to negatively impact germ and somatic cells in the testis. Physical agents, such as ionizing radiation, were also described to perturb spermatogenesis. Despite the fact that we are constantly exposed to numerous environmental chemical and physical compounds, very few studies explore the impact of combined exposure to chemical and physical pollutants on reproductive health. The aim of this study was to describe the impact of fetal co-exposure to BPA and IR on testicular function in mice. We exposed pregnant mice to 10 µM BPA (corresponding to 0.5 mg/kg/day) in drinking water from 10.5 dpc until birth, and we irradiated mice with 0.2 Gy (γ-ray, RAD) at 12.5 days post-conception. Co-exposure to BPA and γ-ray induces DNA damage in fetal germ cells in an additive manner, leading to a long-lasting decrease in germ cell abundance. We also observed significant alteration of adult steroidogenesis by RAD exposure independently of the BPA exposure. This is illustrated by the downregulation of steroidogenic genes and the decrease of the number of adult Leydig cells. As a consequence, courtship behavior is modified, and male ultrasonic vocalizations associated with courtship decreased. In conclusion, this study provides evidence for the importance of broadening the concept of endocrine disruptors to include physical agents, leading to a reevaluation of risk management and regulatory decisions.

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Differentiation of seminiferous tubule-associated stem cells into leydig cell and myoid cell lineages

Cited by 23 publications

References 31 publications

Single-cell analysis of human testis aging and correlation with elevated body mass index

Single-cell analysis of human testis aging and correlation with elevated body mass index

The Molecular Signature of Human Testicular Peritubular Cells Revealed by Single-Cell Analysis

Unexpected Interacting Effects of Physical (Radiation) and Chemical (Bisphenol A) Treatments on Male Reproductive Functions in Mice

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