Differentiation of Sparidae species by DNA sequence analysis, PCR-SSCP and IEF of sarcoplasmic proteins

Schiefenhövel, Karin; Rehbein, Hartmut

doi:10.1016/j.foodchem.2012.10.057

Cited by 17 publications

(11 citation statements)

References 19 publications

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“…In a former PCR-SSCP study, seven fi sh species from Sparidae family were included and fi ve of them were successfully differentiated from each other (SCHIEVENHÖVEL & REHBEIN, 2013). SRIPHAIROJ and co-workers (2010) have been conducted a successful species identifi cation work on 5 Pangasiid species by PCR-SSCP.…”

Section: Pcr-single Strand Conformation Polymorphismmentioning

confidence: 99%

Applicability and sensitivity of PCR-SSCP method for milk species identification in cheese

Csikós¹,

Hodžić

Pasic-Juhas

et al. 2016

Acta Alimentaria

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Species identifi cation in food has become a prominent issue in recent years as the importance of consumer protection has increased. DNA-based species identifi cation methods were developed by researchers in the last two decades, as these are reliable, accurate, and low-cost techniques for species identifi cation in raw and processed food products as well. In our study, universal primers were designed to conserved regions of mitochondrial 12S rRNA. Amplicons were heat-denatured and a PCR single strand conformation polymorphism (SSCP) method was developed to identify cattle, buffalo, sheep, and goat DNA. Sensitivity of this technique was tested on DNA mixtures of cattle-sheep, cattle-goat, and cattle-buffalo and the threshold limit of cattle DNA was 5%, 5%, and 3%, respectively. One hundred and fi ve cheeses were purchased and collected from Bosnian and Hungarian farmers, retails, and supermarkets to reveal fraud, 32 percent of them (34 cheeses) were found to be mislabelled by species.

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Section: Pcr-single Strand Conformation Polymorphismmentioning

confidence: 99%

Applicability and sensitivity of PCR-SSCP method for milk species identification in cheese

Csikós¹,

Hodžić

Pasic-Juhas

et al. 2016

Acta Alimentaria

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“…Seafood authentication in food control laboratories can be carried out by a number of methods that include protein-based electrophoretic and mass spectroscopy techniques (Schiefenh€ ovel & Rehbein, 2013;Wulff et al, 2013;Carrera et al, 2014) or DNAbased methods (Lockley & Bardsley, 2000;Rasmussen & Morrissey, 2008;Handy et al, 2011). Moreover, the authorities or research institutions responsible for food quality assurances are often confronted with new imported food products.…”

Section: Introductionmentioning

confidence: 99%

DNA barcoding for the species identification of commercially important fishery products in Indonesian markets

Abdullah

Rehbein

2016

Int J of Food Sci Tech

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Summary The DNA barcoding approach was used for the species identification of 44 Indonesian commercial fishery products. Additionally, the intronless nuclear rhodopsin gene fragment (RH1) was added to the analysis to enable the identification of species not yet barcoded and possible hybrids. The 655‐bp cytochrome C oxidase subunit I (COI) gene fragment marker was successfully amplified and used to identify 86% of the total fish samples at the species level using the BOLD and BLAST public databases. Moreover, the RH1 marker was used to complete COI analysis. For a number of fish species, the COI sequences (six species) and RH1 sequences (eight species) were the first entries submitted to GenBank. This study demonstrated that COI barcoding is a promising tool for Indonesian fishery products and confirmed that it could be adopted in the future for regular seafood control as part of the Indonesian integrated food traceability system.

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“…Electrophoretic, chromatographic and immunological methods of protein analysis have been pushed to the background due to the greater stability and higher information content of DNA [16]. However, isoelectric focusing (IEF) of sarcoplasmic proteins is still in use as a rapid, low-cost and reliable screening method for the identification of raw or breaded fish products [17][18][19][20][21]. Recently, proteomics (twodimensional electrophoresis combined with mass spectroscopy) has been used as an additional tool for fish species identification, e.g.…”

Section: Introductionmentioning

confidence: 99%

Authentication of closely related scombrid, catfish and tilapia species by PCR-based analysis and isoelectric focusing of parvalbumin

Abdullah

Rehbein

2015

Eur Food Res Technol

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Differentiation of Sparidae species by DNA sequence analysis, PCR-SSCP and IEF of sarcoplasmic proteins

Cited by 17 publications

References 19 publications

Applicability and sensitivity of PCR-SSCP method for milk species identification in cheese

Applicability and sensitivity of PCR-SSCP method for milk species identification in cheese

DNA barcoding for the species identification of commercially important fishery products in Indonesian markets

Authentication of closely related scombrid, catfish and tilapia species by PCR-based analysis and isoelectric focusing of parvalbumin

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