Differentiation of Staphylococcal Species and Strains by Ribosomal RNA Gene Restriction Patterns

Thomson-Carter, Fiona M.; Carter, P. E.; Pennington, T. H.

doi:10.1099/00221287-135-7-2093

Cited by 66 publications

(57 citation statements)

References 14 publications

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“…In our study, the rDNA fingerprinting technique, which has been applied in recent years to many bacterial species [7][8][9][10], was used on strains of S. typhimurium isolated over the last 10 years in Italy from human and non-human sources, in order to investigate the epidemiological features of interchange of clones between man and animal hosts.…”

Section: Introd)uctionmentioning

confidence: 99%

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Epidemiology ofSalmonella typhimurium: ribosomal DNA analysis of strains from human and animal sources

1993

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SUMMARYSalmonella typhimurium is the most frequently identified serovar of Salmonella in Italy. This serovar is characterized by the widespread dissemination among human and non-human sources of phenotypically and genetically well-differentiated clones.In this study 457 strains of S. typhimurium isolated in Italy in the years 1982-91 from human and animal sources were submitted to characterization by the rDNA fingerprinting technique. Application of this typing method, after digestion of chromosomal DNA with HincIl endonuclease, confirmed the greatest genetic differentiation of clones of S. typhimurium, allowing reliable identification of 45 rDNA patterns linked into 9 major clusters. rDNA pattern clusters or ribotypes specific to man were not recognized, whereas some rDNA patterns were characteristically related to ducks, pigeons and pet birds. The ribotyping results for isolates from animal hosts suggest that pig and cattle are the main source of human infection. INTROD)UCTIONSalmonella typhimuriumn is the most frequently identified serovar of Salmonella at the Centres of Enterobacteriaceae in Italy. This serovar, which is characterized by a widespread dissemination and by its differentiation in phenotypically and genetically distinct clones, has been studied by various typing techniques to obtain reliable strain discrimination. In particular, combined use of biotyping and phage typing [1] and plasmid profile analysis [2,3] have proved to be useful tools in tracing of S. typhimurium infections.Because of the involvement of man and of a wide range of animal hosts in the spread of this serovar, possible relationships between bio-phage types of strains of S. typhimurium and sources have been investigated [4][5][6]. In our study, the rDNA fingerprinting technique, which has been applied in recent years to many bacterial species [7][8][9][10], was used on strains of S. typhimurium isolated over the last 10 years in Italy from human and non-human sources, in order to investigate the epidemiological features of interchange of clones between man and animal hosts. 19HYG 110

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Section: Introd)uctionmentioning

confidence: 99%

“…In our study, the rDNA fingerprinting technique, which has been applied in recent years to many bacterial species [7][8][9][10], was used on strains of S. typhimurium isolated over the last 10 [7,11,12]. The hybridized bands were visualized by the 'Blugene' non-radioactive nucleic acid detection system (Gibco-BRL.…”

Section: Introd)uctionmentioning

confidence: 99%

Epidemiology ofSalmonella typhimurium: ribosomal DNA analysis of strains from human and animal sources

1993

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“…Many other techniques have been used to investigate isolates of S. aureus, such as peptidoglycan f i n g e~r i n t i n g ,~~ immunoblotting of exported proteins and restriction enzyme fragmentation patterns,26 penicillin-binding protein analysis2' and ribotyping. 28 Further work is required to assess the suitability of these techniques for CNS. Most recently the typing of CNS by pyrolysis mass spectrometry has shown promise.29…”

Section: Discussionmentioning

confidence: 99%

Evaluation of methods for typing coagulase-negative staphylococci

Hg²,

Martin³

et al. 1992

Journal of Medical Microbiology

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Summary.One hundred and forty-two coagulase-negative staphylococci (CNS) isolated from dialysate effluent or skin of patients receiving continuous ambulatory peritoneal dialysis (CAPD) were typed by extended antibiogram (16 antibiotics) and biotype (26 reactions). These isolates were then typed by supplementary methods to determine the most suitable typing method for an epidemiological study of antibiotic resistance. These included phage typing, reverse phage typing, plasmid typing, whole-cell protein typing by SDS-PAGE with analysis by densitometry, and immunoblotting. The percentage of isolates typed successfully by the supplementary methods were: phage typing 20 %, reverse phage typing 0 %, plasmid typing 66 %, SDS-PAGE 100 %, immunoblotting 100 YO. The discrimination of each method was : phage typing 20 YO, plasmid typing 37 %, SDS-PAGE 69 %, immunoblotting 57 YO.Reproducibility was 88 % for phage typing and 97 % for plasmid typing. The reproducibility of the whole-cell protein typing was 83% if the same extracts were used but only 43 YO when separate protein extracts were analysed on separate occasions. However, strain relatedness was highly reproducible. The determination of an antibiogram-biotype profile was not a sufficiently accurate typing method for an epidemiological study of antibiotic resistance. Whole-cell protein typing by SDS-PAGE or immunoblotting was technically demanding but was the most effective of the supplementary methods for detecting erroneous discrimination and false matching produced by antibiogram-biotype combinations.

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“…Studies on a variety of micro-organisms including Haemophilus infiuenzae (Irino et al 1988), Staphylococcus spp. (De Buyser et al 1989;Thomson-Carter et al 1989) and Streptococcus thermophilus (Salzano et al 1994) have demonstrated that rRNA gene restriction pattern analysis provides a means of distinguishing between strains at species, subspecies or other taxonomic levels. The study of polymorphism of ribosomal DNA, facilitated by restriction analysis of rRNA genes amplified by polymerase chain reaction (ARDRA-PCR), has also been used for the differentiation of species and subspecies (Gurtler et al 1991;Jayarao et ul.…”

Section: Introductionmentioning

confidence: 99%

Characterization of strains ofLeuconostoc mesenteroidesby analysis of soluble whole-cell protein pattern, DNA fingerprinting and restriction of ribosomal DNA

Villani¹,

Moschetti²,

Blaiotta³

et al. 1997

Journal of Applied Microbiology

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Of 215 leuconostocs isolated from field grass, natural whey cultures and water‐buffalo milk, 178 were identified as Leuconostoc mesenteroides ssp. mesenteroides while 37 strains could not be identified Biochemical characterization allowed seven groups to be defined. Representative strains of each group and different habitat and nine reference strains were selected for further analyses. Protein profiles appeared suitable for species discrimination, but did not differentiate between the three subspecies of Leuc. mesenteroides. The technique also showed some differences among equivocal strains. DNA fingerprinting for most strains of Leuc. mesenteroides ssp. mesenteroides examined showed a different restriction pattern from that of the type strain. Ribotyping was not useful for discriminating species and subspecies of the genus Leuconostoc: Leuc. mesenteroides ssp. mesenteroides and ssp. dextranicum showed the same ribopattern as Leuc. lactis while Leuc. mesenteroides ssp. cremoris exhibited a pattern distinct from all the other species examined. On the basis of ARDRA‐PCR, two main groups could be distinguished: the larger group included Leuc. mesenteroides, Leuc. lactis, Leuc. pseudomesenteroides and some unidentifiable strains; the second one included Leuc. citreum, Leuc. fallax, Weissella paramesenteroides and some unidentified strains.

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Differentiation of Staphylococcal Species and Strains by Ribosomal RNA Gene Restriction Patterns

Cited by 66 publications

References 14 publications

Epidemiology ofSalmonella typhimurium: ribosomal DNA analysis of strains from human and animal sources

Epidemiology ofSalmonella typhimurium: ribosomal DNA analysis of strains from human and animal sources

Evaluation of methods for typing coagulase-negative staphylococci

Characterization of strains ofLeuconostoc mesenteroidesby analysis of soluble whole-cell protein pattern, DNA fingerprinting and restriction of ribosomal DNA

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