Differentiation of steroid-producing cells during ovarian differentiation in the protogynous Malabar grouper, Epinephelus malabaricus

Murata, Ryosuke; Karimata, Hirofumi; Kobayashi, Yasuhisa; Horiguchi, Ryo; Kishimoto, Kazuo; Kimura, Motofumi; Kobayashi, Tohru; Soyano, Kiyoshi; Nakamura, Masaru

doi:10.1387/ijdb.103181rm

Cited by 24 publications

(13 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similar results were reported for the MT treatment of immature Malabar groupers [ 31 ]. A recent study suggests that there are two types of steroid-producing cells, estrogen-producing cells, and androgen-producing cells in the gonads of the immature Malabar groupers [ 36 ], demonstrating that immature groupers have a chaotic endocrine system. MT, as an androgen, may directly stimulate spermatogenesis; however, large numbers of androgen-producing cells may not be produced during MT feeding, which may explain why MT treatment at this stage cannot induce permanent sex reversal.…”

Section: Discussionmentioning

confidence: 99%

MT-Feeding-Induced Impermanent Sex Reversal in the Orange-Spotted Grouper during Sex Differentiation

Wang

Huang

Peng

et al. 2018

IJMS

View full text Add to dashboard Cite

In this study, we systematically investigated the process of sex reversal induced by 17-methyltestosterone (MT) feeding and MT-feeding withdrawal at the ovary differentiation stage in orange-spotted groupers, Epinephelus coioides. Gonadal histology showed that MT feeding induced a precocious sex reversal from immature ovaries to testes, bypassing the formation of an ovarian cavity, and MT-feeding withdrawal led to an ovarian fate. In both the MT feeding and MT-feeding withdrawal phases, cytochrome P450 family 11 subfamily B (cyp11b) gene expression and serum 11-KT levels were not significantly changed, suggesting that the MT-treated fish did not generate endogenous steroids, even though active spermatogenesis occurred. Finally, by tracing doublesex-expressing and Mab-3-related transcription factor 1 (dmrt1)-expressing cells and TUNEL (terminal deoxynucleotidyl transferase 2-deoxyuridine, 5-triphosphate nick end labeling) assays, we found that the efferent duct formed first, and then, the germ cells and somatic cells of the testicular tissue were generated around the efferent duct during MT-feeding-induced precocious sex reversal. Collectively, our findings provide insights into the molecular and cellular mechanisms underlying sex reversal induced by exogenous hormones during sex differentiation in the protogynous orange-spotted grouper.

show abstract

Section: Discussionmentioning

confidence: 99%

MT-Feeding-Induced Impermanent Sex Reversal in the Orange-Spotted Grouper during Sex Differentiation

Wang

Huang

Peng

et al. 2018

IJMS

View full text Add to dashboard Cite

show abstract

“…Honeycomb grouper and Malabar grouper, Cyp11c1 immunoreactive cells were only observed in the vicinity of blood vessels at the periphery of the ovary(Alam et al, 2005;Alam et al, 2006;Murata et al, 2011). Recently, in the ovary of a protandrous anemonefish, a signal was detected in theca cells surrounding developed oocytes and in vitro experiments showed the ability of the female-phase ovary to synthesize a small amount of 11-ketotestosterone(Miura et al, 2008).…”

mentioning

confidence: 99%

Localization of steroidogenic enzymes and Foxl2a in the gonads of mature zebrafish ( Danio rerio )

Caulier¹,

Brion²,

Chadili³

et al. 2015

Comparative Biochemistry and Physiology Part A: Molecular &

View full text Add to dashboard Cite

In zebrafish, the identification of the cells expressing steroidogenic enzymes and their regulators is far from completely fulfilled though it could provide crucial information on the elucidation of the role of these enzymes. The aim of this study was to better characterize the expression pattern of steroidogenic enzymes involved in estrogen and androgen production (Cyp17-I, Cyp11c1, Cyp19a1a and Cyp19a1b) and one of their regulators (Foxl2a) in zebrafish gonads. By using immunohistochemistry, we localized the steroid-producing cells in mature zebrafish gonads and determined different expression patterns between males and females. All these steroidogenic enzymes and Foxl2a were detected both in the testis and ovary. In the testis, they were all localized both in Leydig and germ cells except Cyp19a1b which was only detected in germ cells. In the ovary, Cyp17-I, Cyp19a1a and Foxl2a were immunolocalized in both somatic and germ cells while Cyp19a1b was only detected in germ cells and Cyp11c1 in somatic cells. Moreover, Cyp19a1a and Foxl2a did not display exactly the same patterns of spatial localization but their expressions were correlated suggesting a possible regulation of cyp19a1a gene by Foxl2a in zebrafish. Comparative analysis revealed a dimorphic expression of Cyp11c1, Cyp19a1a, Cyp19a1b and Foxl2a between males and females. Overall, our study provides a detailed description of the expression of proteins involved in the biosynthesis of steroidal hormones at the cellular scale within gonads, which is critical to further elucidating the intimate roles of the enzymes and the use of the zebrafish as a model in the field of endocrinology.

show abstract

“…Сравнительный анализ секреторной активности у самок всех видов круглоротых и рыб показал, что происходило последовательное её смещение из стромы сначала в гранулезу, а затем и в теку фолликулов ооцитов в ходе развития яичников. Последовательное появление и начало функционирования различных типов стероидсекреторных клеток в ходе дифференцировки пола ранее было показано у некоторых видов костистых рыб -у кижуча Oncorhynchus kisutch (Walbaum, 1792) (Мосягина, Зеленников, [Mosyagina and Zelennikov] 2012), тиляпии Sarotherodon niloticus (Linnaeus, 1758) (Nakamura and Nagahama 1985) и группера Epinephelus malabaricus (Bloch et Schneider, 1801) (Murata et al 2011).…”

Section: результаты и обсуждениеunclassified

“…К настоящему времени в литературе накоплено много сведений о состоянии стероидсекреторных клеток в гонадах у молоди (Арбузова [Arbuzova] 1996; Федоров и др. [Fedorov et al] 1990;Cavaco et al, 1999;Devlin and Nagahama 2002;Kobayashi et al, 2011; Мосягина и др. [Mosyagina et al] 2003;Мосягина [Mosyagina] 2006;Nakamura et al 1998) и взрослых особей круглоротых и рыб (Alam et al 2005;Do Rego and Vaudry 2016;Guraya 1976aGuraya , 1976b; Vizziano-Cantonnet 2018; и др.).…”

Section: Introductionunclassified

Special features of ultrastructural organization steroid-secretory cells in the gonads of juveniles of cyclostomes and fishes

Mosyagina

Zelennikov

2019

Proceedings ZIN

View full text Add to dashboard Cite

The comparative ultrastructural analysis of steroid-secretory cells (SC) in gonads of youngs lamprey Lampetra fluviatilis (Linnaeus 1758), Siberian sturgeon Acipenser baerii Brandt 1869, sterlet A. ruthenus Linnaeus, 1758, rainbow trout Oncorhynchus mykiss (Walbaum 1792) and pink salmon Oncorhynchus gorbuscha (Walbaum 1792) at similar stages of gametogenesis was carried out. The SC localization and size, the diameters of mitochondria, tubules of agranular reticulum, lipid inclusions and also the relative volume density of these structures were determined and calculated. It is concluded that localization of SC in gonads and their activity changes are closely related to the processes of sex differentiation and the growth of oocytes. Thus, in females of all studied species there was a change in SC localization in the direction of stroma→theca→granulosa of previtellogenic oocytes with a simultaneous increase of their secretory activity (increase in SC size, size and volume density of mitochondria and of agranular endoplasmic reticulum tubules). In males, during gametogenesis, the SC localization also changed in the direction of epithelium→stroma testicular with an increase of their secretory activity. In this case, the largest and most active SC were found in the stroma of pink salmon testicular during the natural sex inversion. At the same time, however, were revealed differences in SC ultrastructural organization, appeared to be of species-specific nature. The highest average diameter of the agranular endoplasmic reticulum tubules was observed in sturgeon SC and they are characterized by a large number of lipid inclusions in early stages of gametogenesis. This should be taken into account when comparatively analysing of quantitative data in fish of different systematic groups.

show abstract

Differentiation of steroid-producing cells during ovarian differentiation in the protogynous Malabar grouper, Epinephelus malabaricus

Cited by 24 publications

References 0 publications

MT-Feeding-Induced Impermanent Sex Reversal in the Orange-Spotted Grouper during Sex Differentiation

MT-Feeding-Induced Impermanent Sex Reversal in the Orange-Spotted Grouper during Sex Differentiation

Localization of steroidogenic enzymes and Foxl2a in the gonads of mature zebrafish ( Danio rerio )

Special features of ultrastructural organization steroid-secretory cells in the gonads of juveniles of cyclostomes and fishes

Contact Info

Product

Resources

About