Differentiation of two Maytenus species and their hybrid via untargeted metabolomics

Antunes, Elisa Ribeiro Miranda; Duarte, Rodolfo Santos; Moritz, Thomas; Sawaya, Alexandra Christine Helena Frankland

doi:10.1016/j.indcrop.2020.113014

Cited by 4 publications

(4 citation statements)

References 38 publications

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“…Dilutions of this working solution in purified water were used for method validation and quantification. Other components cited in Figure 2 were putatively identified in a previous study [ 9 ].…”

Section: Methodsmentioning

confidence: 74%

“…In the present work, metabolomics was associated with quantification to develop a better quality-control method for M. ilicifolia , using a pool of authenticated individuals as standards for these species. A previous study with M. ilicifolia , M. aquifolium and hybrid individuals showed that their composition was not strongly affected by the seasons [ 9 ]; therefore, the pooled leaves that were used can be considered representative of the composition of these species.…”

Section: Resultsmentioning

confidence: 99%

“… Heatmap showing the 20 most significant features of the UHPLC-MS results, samples identified according to Table 2 . Asterisks (*) indicate the compounds identified in a previous study [ 9 ]. …”

Section: Figurementioning

confidence: 99%

“…Metabolomics is an approach focused on the analysis of the metabolism of organisms and it is usually subdivided into two techniques: targeted and untargeted. Targeted metabolomics aims to detect and identify specific target compounds while untargeted metabolomics focuses on detecting as many compounds as possible, providing a general view of the organism’s metabolism [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous UHPLC-MS Quantification of Catechins and Untargeted Metabolomic Profiling for Proof-of-Concept Authenticity Determination of Maytenus ssp. Samples

2022

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Due to the widespread use of Maytenus ilicifolia leaves in the treatment of gastric ulcers, herbal medicines derived from such species are distributed by the national health system in Brazil. A related species, Maytenus aquifolium, is also used for the same disorders, and both are popularly known as Espinheira-santa. Due to their popular use, the quality and efficiency of the herbal medicines derived from these species is an important public health issue. The purpose of this study was to develop and test an analytical method that could quantify the content of catechin and epicatechin in dry Maytenus spp. leaves and simultaneously obtain their chemical profile to determine authenticity of the leaf samples. Ultra-high performance liquid chromatography coupled to mass spectrometry (UHPLC-MS) was used to quantify these isomers, in the selected ion monitoring (SIM) mode, while simultaneously analyzing the extract in full-scan mode. This approach was successfully applied to the analysis of commercial and authentic samples of Maytenus spp. Fewer than half the samples presented the minimum epicatechin content of 2.8 mg per g of dry leaf mass, as specified in the 6th Brazilian Pharmacopoeia (2019) for M. ilicifolia. Furthermore, by using untargeted metabolomics, it was observed that the chemical profile of most the samples was not compatible with M. ilicifolia leaves, indicating the need for stricter quality control of this material. The method described herein could be used for this control; moreover, its concept could be adapted and used for an ample variety of medicinal plant products.

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Section: Methodsmentioning

confidence: 74%

Section: Resultsmentioning

confidence: 99%