Differentiation-related expression of the Thomsen-Friedenreich glycotope in developing human lung and in lung carcinoma

Toma, Valeriu; Sata, Tetsutaro; Vogt, Peter R.; Komminoth, Paul; Heitz, Philipp U.; Roth, Jürgen

doi:10.1002/(sici)1097-0142(19990515)85:10<2151::aid-cncr9>3.0.co;2-3

Cited by 7 publications

(8 citation statements)

References 34 publications

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“…Four cases revealed over 50% positive cells. These data confirm the expression of CD176 in lung cancer tissues in contrast to an earlier paper (Toma et al. 1999).…”

Section: Resultssupporting

confidence: 89%

“…The CD176 antigen has been found to be a useful marker of prognosis in lung patients (Takanami 1999), but there remain conflicting reports on its expression in this tissue (Toma et al. 1999).…”

Section: Discussionmentioning

confidence: 99%

“…A by‐product of this study was the resolution of conflicting reports on the expression of CD176 in lung carcinomas (Takanami 1999; Toma et al. 1999).…”

mentioning

confidence: 94%

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Expression of CD176 (Thomsen-Friedenreich antigen) on lung, breast and liver cancer-initiating cells

Lin

Karsten

Goletz

et al. 2010

International Journal of Experimental Pathology

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The cancer-initiating capacity of most malignant tumours is considered to reside in a small subpopulation of cells. Therapeutical interventions should target these cells rather than the tumour mass. Numerous studies have shown that the carbohydrate antigen structure CD176 (Thomsen-Friedenreich antigen, core-1) is present in many types of cancer and absent in normal adult human tissues. In this study, we assessed whether CD176 is co-expressed with CD44 or CD133 [markers of cancer-initiating cells (CIC)] in human lung, breast and liver carcinoma. A variety of human cancer cell lines and surgical specimens of these malignancies were examined. It was found that in most cases the majority of tumour cells stained strongly for CD44 by immunohistochemistry and flow cytometry, whereas CD133 expression was found on a smaller, but varying proportion of cells. Co-expression of CD176 with CD44 was found at a surprisingly high percentage of cancer cells in vitro and in vivo. Co-expression of CD176 with CD133 was also detected, although at a lower rate. Tamoxifen treatment of MDA-435 breast cancer cells enhanced the CD44(+) /CD176(+) phenotype. Evidence is provided through a new sandwich solid-phase enzyme-linked immunosorbent assay (ELISA) suggesting that CD44 is a carrier molecule for CD176 not only in colorectal cancer as previously reported, but also in lung, breast and liver cancer. The expression of CD176 in CIC suggests that it may represent an effective target for tumour therapies.

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“…Four cases revealed over 50% positive cells. These data confirm the expression of CD176 in lung cancer tissues in contrast to an earlier paper (Toma et al. 1999).…”

Section: Resultssupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

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Expression of CD176 (Thomsen-Friedenreich antigen) on lung, breast and liver cancer-initiating cells

Lin

Karsten

Goletz

et al. 2010

International Journal of Experimental Pathology

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“…This also applies to the detection of glycans with antibodies, and to glycans as complex as polysialic acid present on the neural cell adhesion molecule NCAM (Komminoth et al 1991;Roth et al 1987Roth et al , 1988. Although desirable to be carried out under controlled conditions, aldehyde fixation using 10% buffered formalin for varying periods of time followed by routine paraffin embedding, as done in surgical pathology laboratories, provides a suitable material for lectin labeling (Murayama et al 1997;Sata et al 1992;Seelentag et al 1998;Toma et al 1999). As an example, the detection of b1,6-branched N-glycans with the leukoagglutinating Phaseolus vulgaris lectin (Cummings and Kornfeld 1982) in normal human duodenum from a routinely formalin-fixed and paraffin-embedded surgical specimen is shown in Fig.…”

Section: Aldehyde Fixationmentioning

confidence: 99%

Lectins for histochemical demonstration of glycans

Roth

2011

Histochem Cell Biol

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Lectins have been proven to be invaluable reagents for the histochemical detection of glycans in cells and tissues by light and electron microscopy. This technical review deals with the conditions of tissue fixation and embedding for lectin labeling, as well as various markers and related labeling techniques. Furthermore, protocols for lectin labeling of sections from paraffin and resin-embedded tissues are detailed together with various controls to demonstrate the specificity of the labeling by lectins.

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“…The suitability of all these lectins recognizing NeuAc5 in a specific glycosidic linkage for the histochemical detection of the respective binding sites in tissues was subsequently demonstrated (Sata et al 1989(Sata et al , 1990aTaatjes et al 1988;Yamashita et al 1995). Noteworthy, all these lectins proved to be of practical importance in cancer research (Benallal et al 1995;Boland et al 1991;Lemarer and Stehelin 1995;Murayama et al 1997;Pousset et al 1997;Sata et al 1991Sata et al , 1992Toma et al 1999Toma et al , 2000Vierbuchen et al 1995;Yamashita et al 1995).…”

Section: Introductionmentioning

confidence: 99%

Application of a lectin from the mushroom Polysporus squamosus for the histochemical detection of the NeuAcα2,6Galβ1,4Glc/GlcNAc sequence of N-linked oligosaccharides: a comparison with the Sambucus nigra lectin

Toma

Zuber

Winter

et al. 2001

Histochem Cell Biol

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The lectin from the mushroom Polysporus squamosus (PSL) has an extended carbohydrate combining site, which exhibits a high specificity and affinity toward the NeuAc5α2,6Galβ1,4Glc/GlcNAc trisaccharide sequence of asparagine-linked oligosaccharides. Therefore, PSL should be a superior reagent to the lectin from Sambucus nigra (SNA), which does not discriminate between α2,6-linked NeuAc5 present either in asparagineor serine/threonine-linked oligosaccharides. We have prepared a digoxigenin-conjugated PSL and applied it for histochemistry and blotting. We observed a more restricted staining pattern by PSL as compared to SNA in paraffin sections from different rat organs. Pretreatment of sections with N-glycanase F abolished PSL staining indicating that it interacts only with asparagine-linked oligosaccharides. Furthermore, PSL staining was neuraminidase sensitive. In contrast, SNA staining was only partially sensitive to N-glycanase F pretreatment demonstrating that it was in part due to α2,6-linked NeuAc5 present in serine/threonine-linked oligosaccharides. The most striking observation in this regard was that PSL, in contrast to SNA, did not stain the mucus of sheep submandibular gland, which is extremely rich in serine/threonine-linked Neu5Acα2,6N-acetylgalactosamine. Furthermore, in some tissues neuraminidase pretreatment resulted in increased intensity of SNA staining probably due to binding to exposed terminal N-acetylgalactosamine residues. Collectively, these results indicate that PSL is a useful tool for the histochemical detection of α2,6-linked NeuAc5 in asparagine-linked oligosaccharides.Keywords Polysporus squamosus lectin · Sambucus nigra agglutinin · N-glycans · Histochemistry Roth et al. 1984Roth et al. , 1985Schulte et al. 1984;Taatjes and Roth 1991). Since sialic acids occur in specific glycosidic linkages to the penultimate sugar at the non-reducing terminus of oligosaccharide side chains, an important advance for the field was accomplished with the isolation and characterization of lectins with high specificity for sialic acids present in a particular glycosidic linkage. The first reported linkage-specific lectin was the one isolated from Elderberry bark, the Sambucus nigra L. lectin (SNA), recognizing N-acetylneuraminic Application of a lectin from the mushroom Polysporus squamosus for the histochemical detection of the NeuAcα2,6Galβ1,4Glc/GlcNAc sequence of N-linked oligosaccharides: a comparison with the Sambucus nigra lectin

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Differentiation-related expression of the Thomsen-Friedenreich glycotope in developing human lung and in lung carcinoma

Cited by 7 publications

References 34 publications

Expression of CD176 (Thomsen-Friedenreich antigen) on lung, breast and liver cancer-initiating cells

Expression of CD176 (Thomsen-Friedenreich antigen) on lung, breast and liver cancer-initiating cells

Lectins for histochemical demonstration of glycans

Application of a lectin from the mushroom Polysporus squamosus for the histochemical detection of the NeuAcα2,6Galβ1,4Glc/GlcNAc sequence of N-linked oligosaccharides: a comparison with the Sambucus nigra lectin

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