Differentiation-specific expression and localization of an autophagosomal marker protein (LC3) in human epidermal keratinocytes

Haruna, Kunitaka; Suga, Yasushi; Muramatsu, Shigenori; Taneda, Kenichi; Mizuno, Yuki; Ikeda, Shigaku; Ueno, Takashi; Kominami, Eiki; Tanida, Isei; Hanada, Kentaro

doi:10.1016/j.jdermsci.2008.07.005

Cited by 23 publications

(22 citation statements)

References 9 publications

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“…These data indicate that BNIP3 is involved in the induction of autophagy in HPEKs. Intriguingly, these data also confirm the previous finding that autophagosome induction is accompanied by keratinocyte differentiation (Haruna et al, 2008). We observed that the number of mitochondria was decreased in the granular layers, where BNIP3 expression and autophagosome formation was observed ( Figure 4a).…”

Section: Bnip3 Is Required For Terminal Differentiation Of Keratinocysupporting

confidence: 92%

“…Recent reports show that BNIP3 is expressed in mitochondria and that it induces autophagy (Quinsay et al, 2010). In addition, some researchers have reported that autophagy may play a role in epidermal differentiation (Haruna et al, 2008;Aymard et al, 2011;Chatterjea et al, 2011). We therefore investigated whether autophagy occurred in the epidermis, especially in the granular layers.…”

Section: Bnip3 Is Expressed In the Granular Layer Of The Epidermis Wmentioning

confidence: 96%

“…Cornification is also accompanied by activation of lysosomal enzymes and organelle clearance. Moreover, some researchers have reported that autophagy may play a role in epidermal differentiation (Haruna et al, 2008;Aymard et al, 2011;Chatterjea et al, 2011). Therefore, it is likely that BNIP3 is involved in cornification through cell death or autophagy.…”

Section: Introductionmentioning

confidence: 99%

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BNIP3 Plays Crucial Roles in the Differentiation and Maintenance of Epidermal Keratinocytes

Moriyama

Uda

et al. 2014

Journal of Investigative Dermatology

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Transcriptome analysis of the epidermis of Hes1(-/-) mouse revealed the direct relationship between Hes1 (hairy and enhancer of split-1) and BNIP3 (BCL2 and adenovirus E1B 19-kDa-interacting protein 3), a potent inducer of autophagy. Keratinocyte differentiation is going along with activation of lysosomal enzymes and organelle clearance, expecting the contribution of autophagy in this process. We found that BNIP3 was expressed in the suprabasal layer of the epidermis, where autophagosome formation is normally observed. Forced expression of BNIP3 in human primary epidermal keratinocytes (HPEKs) resulted in autophagy induction and keratinocyte differentiation, whereas knockdown of BNIP3 had the opposite effect. Intriguingly, addition of an autophagy inhibitor significantly suppressed the BNIP3-stimulated differentiation of keratinocytes, suggesting that BNIP3 plays a crucial role in keratinocyte differentiation by inducing autophagy. Furthermore, the number of dead cells increased in the human epidermal equivalent of BNIP3 knockdown keratinocytes, which suggests that BNIP3 is important for maintenance of skin epidermis. Interestingly, although UVB irradiation stimulated BNIP3 expression and cleavage of caspase3, suppression of UVB-induced BNIP3 expression led to further increase in cleaved caspase3 levels. This suggests that BNIP3 has a protective effect against UVB-induced apoptosis in keratinocytes. Overall, our data provide valuable insights into the role of BNIP3 in the differentiation and maintenance of epidermal keratinocytes.

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Section: Bnip3 Is Required For Terminal Differentiation Of Keratinocysupporting

confidence: 92%

Section: Bnip3 Is Expressed In the Granular Layer Of The Epidermis Wmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

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BNIP3 Plays Crucial Roles in the Differentiation and Maintenance of Epidermal Keratinocytes

Moriyama

Uda

et al. 2014

Journal of Investigative Dermatology

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“…Sequential differentiation or keratinization is accompanied by the degradation of intracellular constituents and activation of lysosomal enzymes [36]. The differentiation-specific expression and localization of LC3 exhibit in keratinocytes [37]. Series of studies based on autophagy inhibition confirmed the role of autophagy in epidermal keratinization process.…”

Section: Autophagy and Keratinocyte Biology And Pathologymentioning

confidence: 99%

The signaling involved in autophagy machinery in keratinocytes and therapeutic approaches for skin diseases

Chen

2016

Oncotarget

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Autophagy is responsible for the lysosomal degradation of proteins, organelles, microorganisms and exogenous particles. Epidermis primarily consists of keratinocytes which functions as an extremely important barrier. Investigation on autophagy in keratinocytes has been continuously renewing, but is not so systematic due to the complexity of the autophagy machinery. Here we reviewed recent studies on the autophagy in keratinocyte with a focus on interplay between autophagy machinery and keratinocytes biology, and novel autophagy regulators identified in keratinocytes. In this review, we discussed the roles of autophagy in apoptosis, differentiation, immune response, survival and melanin metabolism, trying to reveal the possible involvement of autophagy in skin aging, skin disorders and skin color formation. Since autophagy routinely plays a double-edged sword role in various conditions, its functions in skin homeostasis and potential application as a therapeutic target for skin diseases remains to be clarified. Furthermore, more investigations are needed on optimizing designed strategies to inhibit or enhance autophagy for clinical efficacy.

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“…Given the fact that autophagy and RAS are both involved in many physiological and pathophysiological processes as divergent as fetal development, neurodegeneration, ischemic tissue damage, aging, cancer, or keratinocyte differentiation, 8,9 understanding the interaction between RAS and autophagy may be a key to improved understanding not just of hypertrophic or ischemic cardiac disease but also of many more pathological conditions.…”

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confidence: 99%

Angiotensin Receptors and Autophagy

Steckelings

Unger

2009

Hypertension

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Differentiation-specific expression and localization of an autophagosomal marker protein (LC3) in human epidermal keratinocytes

Cited by 23 publications

References 9 publications

BNIP3 Plays Crucial Roles in the Differentiation and Maintenance of Epidermal Keratinocytes

BNIP3 Plays Crucial Roles in the Differentiation and Maintenance of Epidermal Keratinocytes

The signaling involved in autophagy machinery in keratinocytes and therapeutic approaches for skin diseases

Angiotensin Receptors and Autophagy

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