Differing cell population structure reflects differing activity of Percoll-separated pronephros and peritoneal leucocytes from barramundi (Lates calcarifer)

Tumbol, Reiny A.; Baiano, Justice C.F.; Barnes, Andrew C.

doi:10.1016/j.aquaculture.2009.04.030

Cited by 27 publications

(20 citation statements)

References 56 publications

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“…Similar observations have been reported in barramundi (L. calcarifer), Atlantic salmon (Salmo salar) and Atlantic cod (G. morhua) head-kidney cells (Kalgraff et al, 2011;Tumbol et al, 2009). PMA, which bypasses all normal cell surface receptors, triggers leucocytes respiratory burst activity directly at the intracellular side of the membrane through MAP kinase/protein kinase C activated signal transduction (Kudrenko et al, 2009).…”

Section: Direct Stimulation Of Respiratory Burst In Queensland Groupesupporting

confidence: 63%

“…Similarly, two populations were found in the European sea bass (Dicentrarchus labrax) and common carp (Cyprinus carpio) peripheral blood leucocytes (Esteban et al, 2000;Nakayasu et al, 1998), both comprising of similar cell types to those identified in the current study. However, three sub-populations were found in leucocytes derived from the head-kidney of barramundi, Lates calcarifer (Tumbol et al, 2009), dab, Limanda limanda (Skouras et al, 2003) and Atlantic cod, Gadus morhua (Ronneseth et al, 2007). These differences in number of clusters and cellular types will depend on the origin of the cells (head-kidney, peripheral blood, peritoneal cavity), but also on the method used to isolate the cells, the age of the animal and its physiological status.…”

Section: Queensland Grouper Hklmentioning

confidence: 99%

“…For future studies on host pathogen interactions, it will be worthy to use the more mature leucocytes that reside in the peritoneal cavity as these cells compared to head-kidney leucocytes have been shown to be immunologically more relevant (Tumbol et al, 2009).…”

Section: Mature Macrophages and Granulocytes From Queensland Grouper mentioning

confidence: 99%

“…European sea bass (D. labrax) and barramundi (L. calcarifer) macrophages (Sarmento et al, 2004b;Tumbol et al, 2009). …”

Section: Antigen Exposure and Opsonophagocytosismentioning

confidence: 99%

“…, single oxygen, and hypohalites (Tumbol et al, 2009;Secombes, 1990). This complex molecular mechanism by which this variety of ROS are produced is considered to be one of the most important microbicidal component of the cellular innate defence (Stafford et al, 2002;Secombes, 1990).…”

Section: Introductionmentioning

confidence: 99%

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On the origin of Group B Streptococcus from disease outbreaks in wild marine fish in Australia

Deboutteville¹

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Since 2007, 96 wild Queensland groupers, Epinephelus lanceolatus, were found dead from Karumba (Gulf of Carpentaria) in Northern Territory to Brisbane in southern Queensland, Australia. In twelve cases, Streptoccocus agalactiae (Group B Streptococcus, GBS) was isolated in pure culture from eyes and internal organs. GBS has been also isolated from other marine species (Javelin grunter, Giant catfish and Squaretail mullet) and caused an outbreak in three species of wild stingrays (Eastern shovelnose ray, Mangrove whipray and Estuary ray) in a public aquarium also in Queensland. These findings have raised public and industry concern over threats to human health from potential transmission between fish and humans, over environmental impacts on other aquatic animals, and over the potential threat posed to the growing Australian aquaculture industry in which, to date, there have been no reported cases of this disease in spite of the devastation caused to the industry overseas. Therefore, my thesis set out to determine whether GBS isolated from dead wild fish in Australia is indeed virulent in Queensland grouper and causative of mortality, to try to identify from where these infections in wild fish originated, to determine potential risk to human health and to other aquatic animals, and to begin to identify mechanisms by which these Australian isolates may cause fatal disease in Queensland grouper. This information may be employed in future risk assessment of potential human transfer, in mitigating further outbreaks in wild fish through understanding origin of infection, and in future development of preventative measures such as vaccination for farmed fish.In order to confirm that S. agalactiae was the cause of the mortality in wild fish, experimental challenges were conducted by injection, immersion and through the oral route. To better understand why these strains of GBS are pathogenic in fish, we conducted a series of cellular immune assays using juvenile Queensland grouper head-kidney leucocytes in the presence of different piscine and terrestrial isolates. Whole-genome sequencing analysis of the piscine and terrestrial isolates provided information on potential origin and potential for transfer between species, and also informed us on critical virulence factors that could be used during pathogenesis of GBS. Moreover, genome analysis gave intriguing insight into the evolution and adaption of GBS to fish as a host and to life in the aquatic environment. To determine putative origin of GBS in wild fish in Australia, multilocus sequence typing (MLST) and capsular polysaccharide (CPS) were derived from whole-genome sequences and revealed that all piscine isolates from Australia were serotype Ib and fell into ST-261 lineage first identified in 1986 from Tilapia in Israel. Overseas, ST-261 has only been found in fish and poikilotherm animals, and has never been associated with humans. High similarity and identical point mutations in the capsular operon of the piscine isolates suggests a common source of infection...

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Section: Direct Stimulation Of Respiratory Burst In Queensland Groupesupporting

confidence: 63%

Section: Queensland Grouper Hklmentioning

confidence: 99%

Section: Mature Macrophages and Granulocytes From Queensland Grouper mentioning

confidence: 99%

“…European sea bass (D. labrax) and barramundi (L. calcarifer) macrophages (Sarmento et al, 2004b;Tumbol et al, 2009). …”

Section: Antigen Exposure and Opsonophagocytosismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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On the origin of Group B Streptococcus from disease outbreaks in wild marine fish in Australia

Deboutteville¹

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Establishment of a leukocyte cell line derived from peritoneal macrophages of fish, Labeo rohita (Hamilton, 1822)

et al. 2013

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A continuous leukocyte cell line with phagocytic activity was established from peritoneal macrophages of rohu, Labeo rohita (LRPM). LRPM was initiated from adherent mononuclear leukocytes isolated from peritoneal cavity of rohu, without use of any growth factors or feeder cells. These cells exhibited maximum growth at 30 °C in L-15 medium containing 20 % foetal bovine serum, and has been subcultured for more than 60 passages till date. The cells showed 85 % viability after 6 months of storage in liquid nitrogen. The species of origin of the LRPM was confirmed by the amplification and sequencing of 655 bp fragment of cytochrome oxidase subunit I of mitochondrial DNA. Functionally, LRPM showed phagocytic activity of yeast cells and fluorescent latex beads as evaluated by phase contrast and scanning electron microscopy, respectively. Immuno-modulators such as bacterial lipopolysaccharide and phorbol myristate acetate resulted in functional activation of LRPM; and enhanced their microbicidal activity through release of reactive oxygen species and nitric oxide. Culture supernatant from activated cells also revealed lysozyme activity. Cells of LRPM were positive for alpha-naphthyl acetate esterase enzyme indicating macrophage lineage. Our results indicate that this cell line can be a useful in vitro tool to study the role of macrophages in teleost immune system and to evaluate the effects of new aquaculture drugs. The LRPM cell line represents the first reported leukocyte cell line of peritoneal origin from any freshwater species of fish.

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Stimulatory effects of seaweed Laminaria digitata polysaccharides additives on growth, immune-antioxidant potency and related genes induction in Rohu carp (Labeo rohita) during Flavobacterium columnare infection

Abdel-Tawwab,

Harikrishnan,

Devi

et al. 2024

Aquaculture

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Differing cell population structure reflects differing activity of Percoll-separated pronephros and peritoneal leucocytes from barramundi (Lates calcarifer)

Cited by 27 publications

References 56 publications

On the origin of Group B Streptococcus from disease outbreaks in wild marine fish in Australia

On the origin of Group B Streptococcus from disease outbreaks in wild marine fish in Australia

Establishment of a leukocyte cell line derived from peritoneal macrophages of fish, Labeo rohita (Hamilton, 1822)

Stimulatory effects of seaweed Laminaria digitata polysaccharides additives on growth, immune-antioxidant potency and related genes induction in Rohu carp (Labeo rohita) during Flavobacterium columnare infection

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