Difficulties in the molecular diagnosis of helicobacter rodent infections

Poynter, Scott; Phipps, Julian D.; Naranjo-Pino, Angel; Sánchez-Morgado, José M

doi:10.1016/j.vetmic.2008.08.009

Cited by 8 publications

(9 citation statements)

References 18 publications

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“…In 8-and 11-month-old A30P α-syn tg animals, fecal samples were collected from the colon after perfusion, stored on ice, and frozen at −20°C until analyses (Surrey Diagnostics, Cranleigh, UK). After confirmation of Helicobacter species in 16 of 35 tg animals using conventional PCR (Poynter, Phipps, Naranjo-Pino, & Sanchez-Morgado, 2009), positive samples were further analyzed for Helicobacter pylori (H. pylori) (Kawamata et al, 1996). Two samples were also analyzed in order to identify the unknown Helicobacter species using 16S sequencing primers for determination (GSK, UK).…”

Section: Helicobacter Analysesmentioning

confidence: 99%

Early fine motor impairment and behavioral dysfunction in (Thy‐1)‐h[A30P] alpha‐synuclein mice

et al. 2018

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IntroductionIntraneuronal inclusions of alpha‐synuclein are commonly found in the brain of patients with Parkinson's disease and other α‐synucleinopathies. The correlation between alpha‐synuclein pathology and symptoms has been studied in various animal models. In (Thy‐1)‐h[A30P] alpha‐synuclein transgenic mice, behavioral and motor abnormalities were reported from 12 and 15 months, respectively. The aim of this study was to investigate whether these mice also display symptoms at earlier time points.MethodsWe analyzed gait deficits, locomotion, and behavioral profiles in (Thy‐1)‐h[A30P] alpha‐synuclein and control mice at 2, 8, and 11 months of age. In addition, inflammatory markers, levels of alpha‐synuclein oligomers, and tyrosine hydroxylase reactivity were studied.ResultsAlready at 2 months of age, transgenic mice displayed fine motor impairments in the challenging beam test that progressively increased up to 11 months of age. At 8 months, transgenic mice showed a decreased general activity with increased risk‐taking behavior in the multivariate concentric square field test. Neuropathological analyses of 8‐ and 11‐month‐old mice revealed accumulation of oligomeric alpha‐synuclein in neuronal cell bodies. In addition, a decreased presence of tyrosine hydroxylase suggests a dysregulation of the dopaminergic system in the transgenic mice, which in turn may explain some of the motor impairments observed in this mouse model.ConclusionsTaken together, our results show that the (Thy‐1)‐h[A30P] alpha‐synuclein transgenic mouse model displays early Parkinson's disease‐related symptoms with a concomitant downregulation of the dopaminergic system. Thus, this should be an appropriate model to study early phenotypes of alpha‐synucleinopathies.

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Section: Helicobacter Analysesmentioning

confidence: 99%

Early fine motor impairment and behavioral dysfunction in (Thy‐1)‐h[A30P] alpha‐synuclein mice

et al. 2018

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“…The method recommended in this work was the application of PCR‐differential gradient gel electrophoresis to fecal samples reported previously [2]. A PCR assay performed at the low annealing temperature of 50 °C was developed to amplify a 261‐bp sequence spanning two of the seven variable regions in the 16S rDNA gene of Helicobacter species [3]. This assay combined with that of Bohr et al.…”

Section: Detection Methods New Species and Prevalence In Animal Hostsmentioning

confidence: 99%

Helicobacter spp. Other Than Helicobacter pylori

2009

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Non-H. pylori Helicobacter species (NHPHS) are associated with several important human and animal diseases. In the past year research into this group of bacteria has continued to gain attention, and novel species have been described in new niches owing to improvements in detection methods. Polymerase chain reaction and/or sequencing remain the gold standard for the detection of this genus. New insights into the pathogenesis of the NHPHS in hepatobiliary, gastric, and intestinal diseases were gained. In particular, data revealed interaction between hepatic steatosis and infectious hepatitis in the development of hepatocellular carcinoma. Evidence of an association between hepatitis C virus and Helicobacter spp. in hepatocarcinoma development was also provided; and male sex hormone signaling appeared to influence infectious hepatitis induced by Helicobacter hepaticus. More findings support an association between Helicobacter heilmannii and gastric adenocarcinoma; and in mice, mucins MUC4 and MUC5 but not MUC1 influence the colonization and pathogenesis of Helicobacter felis. Data indicated that the roles of the adaptive immune system in H. hepaticus-induced intestinal tumorigenesis are different in the small and large intestines, and environmental factors, such as bile acids may modulate H. hepaticus carcinogenic potential. New reports in the prevention and eradication of NHPHS showed a protective response against Helicobacter suis induced by vaccine administration, and a successful cross-foster rederivation method successfully eradicated Helicobacter spp. from contaminated mice litters. Overall, the studies provided insights into the pathophysiology of Helicobacter species other than Helicobacter pylori.

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“…from 3.5 to 7.5 µm and 0.9 µm in diameter and it has 3 to 8 coils, which makes it two times larger than Helicobacter pylori. After many attempts, the bacterium was finally classified into the Helicobacter family by means of polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH) methods (10,11). Based on 16S rDNA sequence Helicobacter heilmannii was divided in subtype 1 and subtype 2 (12,13).…”

Section: Case Reportmentioning

confidence: 99%

Helicobacter heilmannii associated gastritis: Case report

et al. 2011

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Helicobacter heilmannii (Gastrospirillum hominis) is a small, gram-negative bacterium from Helicobacter family. Its incidence is significantly lower than for Helicobacter pylori and it accounts for about 1% of cases, mainly in countries with lower socioeconomic conditions. Helicobacter heilmannii may be a causative agent for gastritis, gastric ulcer but it can also trigger malignant diseases such as gastric adenocarcinoma and gastric mucosa-associated lymphoid tissue lymphoma. This paper presents our first case of gastritis caused by Helicobacter heilmannii

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Difficulties in the molecular diagnosis of helicobacter rodent infections

Cited by 8 publications

References 18 publications

Early fine motor impairment and behavioral dysfunction in (Thy‐1)‐h[A30P] alpha‐synuclein mice

Early fine motor impairment and behavioral dysfunction in (Thy‐1)‐h[A30P] alpha‐synuclein mice

Helicobacter spp. Other Than Helicobacter pylori

Helicobacter heilmannii associated gastritis: Case report

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