Ribosome sonification contains total steps of DNA, m-RNA, t-RNA and amino acids consequences that convert the bio-macromolecules data derived from biology systems into acousmatic music are a novel approach in a symphony orchestras. NMR data of the 13 C are particularly well suited data sources for DNA, mRNA and amino acids sonification. One of the important directions in ribosome sonification is time-series-sonification data (TSSD), due to auditory imagination is very sensitive to changes in time. Although, their resonant frequencies are basically in the MHz range, the resonant frequencies span around kHz. The ribosome of E. coli is consisting of several genes which one of them might be divided into 8 series of codons and anti-codons for eight octaves of notes. During NMR calculation with AB-initio methods, these signals are routinely mixed down into the audible frequencies ranges, rendering the need for any additional frequencies transpositions unnecessary. The concert pitches vary from ensemble to ensemble and have varied largely over music methods. The most general advance tuning standard corresponds to 440 Hz for An above middle C as a sequence note. This concept is also applied for distinguishing among the "nominal" (written), and "real" (sounding) notes of a transposing instrument that refers to the sounding pitch on a non-transposing instrument. By this study, E .coli's gene sequences into musical notes for a revealing auditory algorithm has been converted. Estimations of their calculation and optimization of those codons have been done and the total frequencies of each nucleotide have been converted to several music notes and distinguishing those using variations of chemical shifts including pitch, time duration length of notes and even rhythm have been accomplished.